##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_9_ACTTGA_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	26710200	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.081450007862166	34.0	33.0	34.0	31.0	34.0
2	33.27240522347268	34.0	34.0	34.0	31.0	34.0
3	33.275997596423835	34.0	34.0	34.0	31.0	34.0
4	36.58086480071284	37.0	37.0	37.0	35.0	37.0
5	36.527118741155064	37.0	37.0	37.0	35.0	37.0
6	36.47625120740391	37.0	37.0	37.0	35.0	37.0
7	36.50857634911008	37.0	37.0	37.0	35.0	37.0
8	36.477019902509156	37.0	37.0	37.0	35.0	37.0
9	38.32353067367522	39.0	39.0	39.0	37.0	39.0
10	38.30660073679718	39.0	39.0	39.0	37.0	39.0
11	38.28824449086866	39.0	39.0	39.0	37.0	39.0
12	38.25162226415377	39.0	39.0	39.0	37.0	39.0
13	38.25660871127884	39.0	39.0	39.0	37.0	39.0
14	39.79166984897155	41.0	40.0	41.0	38.0	41.0
15	39.78137520497788	41.0	40.0	41.0	38.0	41.0
16	39.76785699845003	41.0	40.0	41.0	38.0	41.0
17	39.719330031224025	41.0	40.0	41.0	38.0	41.0
18	39.658653810154924	41.0	40.0	41.0	37.0	41.0
19	39.66844385291012	41.0	40.0	41.0	37.0	41.0
20	39.642958832206425	41.0	40.0	41.0	37.0	41.0
21	39.60499865220028	41.0	40.0	41.0	37.0	41.0
22	39.53934141264386	41.0	40.0	41.0	37.0	41.0
23	39.51401857717276	41.0	40.0	41.0	37.0	41.0
24	39.48322468570059	41.0	39.0	41.0	37.0	41.0
25	39.431553451490444	41.0	39.0	41.0	36.0	41.0
26	39.318612215558105	41.0	39.0	41.0	36.0	41.0
27	39.220618939581136	41.0	39.0	41.0	36.0	41.0
28	39.148484399218276	41.0	39.0	41.0	35.0	41.0
29	39.075935335564694	40.0	39.0	41.0	35.0	41.0
30	39.00808032137535	40.0	39.0	41.0	35.0	41.0
31	38.967022560669704	40.0	39.0	41.0	35.0	41.0
32	38.896754910109244	40.0	38.0	41.0	35.0	41.0
33	38.828353849840134	40.0	38.0	41.0	35.0	41.0
34	32.32985054398694	40.0	35.0	41.0	2.0	41.0
35	38.68129426211709	40.0	38.0	41.0	35.0	41.0
36	38.61184214270204	40.0	38.0	41.0	35.0	41.0
37	38.76017880809578	40.0	38.0	41.0	35.0	41.0
38	38.77472557300207	40.0	38.0	41.0	35.0	41.0
39	38.753215550613625	40.0	38.0	41.0	35.0	41.0
40	38.71827081040202	40.0	38.0	41.0	35.0	41.0
41	38.6738125135716	40.0	38.0	41.0	35.0	41.0
42	38.60005724404909	40.0	38.0	41.0	35.0	41.0
43	38.53685404826621	40.0	38.0	41.0	35.0	41.0
44	38.46897619635944	40.0	38.0	41.0	35.0	41.0
45	38.40071938809893	40.0	38.0	41.0	35.0	41.0
46	38.306142522332294	40.0	38.0	41.0	34.0	41.0
47	38.2362516192316	40.0	38.0	41.0	34.0	41.0
48	38.18662488487544	40.0	37.0	41.0	34.0	41.0
49	38.12439981729826	40.0	37.0	41.0	34.0	41.0
50	38.19571920090452	40.0	37.0	41.0	34.0	41.0
51	37.92675442340379	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	15.0
10	39.0
11	49.0
12	129.0
13	260.0
14	412.0
15	742.0
16	1138.0
17	1782.0
18	2705.0
19	4482.0
20	6759.0
21	10802.0
22	16685.0
23	23884.0
24	33599.0
25	45554.0
26	60209.0
27	78739.0
28	101461.0
29	130575.0
30	164589.0
31	211191.0
32	271555.0
33	367768.0
34	551381.0
35	959286.0
36	1536320.0
37	2403397.0
38	6144890.0
39	1.3562607E7
40	17186.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.45552049284507	25.519739597917646	28.066311720994854	25.958428188242433
2	22.979821448028556	25.282968142892496	32.45348141896509	19.283728990113854
3	20.8362896517791	24.282249685610953	34.76010868094902	20.121351981660922
4	22.175655742001183	24.353595255745	33.32552358275116	20.145225419502662
5	28.04031044320147	24.524762075911074	27.97923639658258	19.45569108430487
6	21.43669247434177	23.82721140157173	34.172308031907875	20.56378809217862
7	28.113458528951483	24.494653727789384	27.765692506982354	19.62619523627678
8	22.551332449775742	24.652555952407695	32.89750731930124	19.898604278515325
9	21.1211859139954	25.71571908858788	33.23613076652365	19.92696423089307
10	22.232685223768335	24.711761404628003	33.003958444012895	20.051594927590763
11	28.309701911629265	24.465814557734497	27.03943811727355	20.185045413362683
12	22.060409462051712	31.35119961156152	26.24117742176242	20.34721350462435
13	25.264632986649293	25.198130302281523	28.440161436454986	21.09707527461419
14	24.105206647329968	25.595912703839996	28.953063912749215	21.34581673608082
15	24.486293625656117	25.70868806673106	28.433257706793658	21.37176060081916
16	24.51256823236067	25.61671571160081	28.213285561321143	21.657430494717374
17	24.110317406833346	26.126554649534633	28.11620654281885	21.646921400813174
18	23.98012908479811	25.87068817246329	28.597662001256897	21.551520741481696
19	23.38808224388304	26.184295045611776	29.014158320534385	21.413464389970795
20	22.89370727287703	26.265190825976592	29.25607820233469	21.58502369881169
21	23.149984477656286	26.34062356841385	28.879552603073826	21.629839350856034
22	22.89270016697741	26.54241076442707	29.007150826276106	21.557738242319413
23	22.950172593241533	26.624461816085244	28.837286879169753	21.58807871150347
24	22.631185956433473	26.700652014602095	28.882527675446678	21.785634353517754
25	22.739402924725386	26.923770694341485	28.69256314067285	21.644263240260276
26	22.686191410614718	26.873407882641576	28.573685608971918	21.86671509777179
27	22.740512152201227	26.870274009806707	28.47265252092871	21.91656131706336
28	22.71133655180862	26.979239501555153	28.502121081741954	21.807302864894275
29	22.903930345991885	26.93627859596522	28.29239009991429	21.86740095812861
30	22.740411733376142	26.89560369413959	28.426352361745877	21.937632210738386
31	22.87372545953992	26.801644228000526	28.199881235141056	22.124749077318498
32	22.93239153330954	26.977982787421713	28.091679711065243	21.997945968203503
33	22.84620107674222	26.85124783790462	28.045911299803073	22.25663978555009
34	22.83876180640658	27.027907873015796	28.037965985171343	22.09536433540628
35	22.882681346716204	26.904207210494185	27.9538766791224	22.259234763667216
36	22.97965850501858	26.88120564534668	28.011428893201685	22.127706956433055
37	23.037235264901888	26.81594333089155	27.981637740049585	22.16518366415697
38	23.01580746771045	26.839954602828286	27.999485137308323	22.144752792152943
39	22.83883310495616	26.881734318724682	27.962950483335952	22.316482092983208
40	22.865680526540423	26.98761147426826	27.95611039977237	22.19059759941895
41	22.933029329619398	26.8000801192054	28.01298754782817	22.253903003347038
42	22.820842850173317	26.876075271725774	27.925325950560513	22.377755927540395
43	22.84406523667803	26.98857728037791	27.870401815576816	22.296955667367236
44	22.94809473534455	26.778414238755232	27.864400116809307	22.40909090909091
45	23.026484437239354	26.741036428151872	27.78186590492846	22.45061322968031
46	22.846076009819733	26.972584800140144	27.83492452290427	22.346414667135857
47	22.862000284535497	27.016911142559767	27.77277594327261	22.348312629632126
48	22.808979934349356	26.806527984674606	27.779104987451785	22.605387093524254
49	23.01408536866386	26.817538873446807	27.781114622171106	22.387261135718234
50	22.85036156277546	26.806683198046187	27.720245838126868	22.622709401051484
51	22.911710460491285	26.83919724080073	27.672517532717794	22.57657476599019
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.413948681087504
2	42.26355043814241
3	40.95764163344002
4	42.32088116150384
5	47.49600152750635
6	42.00048056652039
7	47.73965376522826
8	42.449936728291064
9	41.04815014488847
10	42.2842801513591
11	48.49474732499195
12	42.407622966676065
13	46.36170826126349
14	45.451023383410785
15	45.85805422647528
16	46.169998727078045
17	45.757238807646516
18	45.53164982627981
19	44.80154663385383
20	44.47873097168872
21	44.77982382851232
22	44.45043840929682
23	44.538251304745
24	44.41682030995123
25	44.38366616498566
26	44.552906508386506
27	44.65707346926459
28	44.5186394167029
29	44.771331304120494
30	44.67804394411453
31	44.99847453685842
32	44.93033750151304
33	45.10284086229231
34	44.93412614181286
35	45.14191611038342
36	45.10736546145163
37	45.20241892905886
38	45.16056025986339
39	45.155315197939366
40	45.05627812595937
41	45.186932332966435
42	45.19859877771371
43	45.14102090404527
44	45.35718564443546
45	45.47709766691967
46	45.192490676955586
47	45.21031291416762
48	45.41436702787362
49	45.40134650438209
50	45.47307096382695
51	45.48828522648148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157768.0
1	90104.0
2	22440.0
3	26810.5
4	31181.0
5	82591.0
6	134001.0
7	207721.0
8	281441.0
9	163076.0
10	44711.0
11	48163.0
12	51615.0
13	49923.5
14	48232.0
15	62674.0
16	77116.0
17	106503.0
18	135890.0
19	162074.0
20	188258.0
21	216006.5
22	243755.0
23	290484.0
24	337213.0
25	393549.0
26	520014.5
27	590144.0
28	641489.0
29	692834.0
30	742620.5
31	792407.0
32	862822.5
33	933238.0
34	998963.5
35	1064689.0
36	1163673.5
37	1262658.0
38	1333199.0
39	1403740.0
40	1474516.5
41	1545293.0
42	1599404.5
43	1653516.0
44	1698010.5
45	1742505.0
46	1749913.5
47	1757322.0
48	1752940.5
49	1748559.0
50	1712880.5
51	1677202.0
52	1582615.0
53	1488028.0
54	1395683.5
55	1303339.0
56	1201489.0
57	1099639.0
58	996467.5
59	893296.0
60	805402.0
61	717508.0
62	624624.5
63	531741.0
64	461780.5
65	391820.0
66	332737.5
67	273655.0
68	254879.0
69	236103.0
70	208156.5
71	180210.0
72	152593.5
73	124977.0
74	105279.5
75	71965.0
76	58348.0
77	55847.5
78	53347.0
79	54194.0
80	55041.0
81	48709.0
82	42377.0
83	39105.5
84	35834.0
85	33145.0
86	30456.0
87	30503.5
88	30551.0
89	19391.5
90	8232.0
91	4923.5
92	1615.0
93	1158.0
94	701.0
95	421.5
96	142.0
97	88.5
98	35.0
99	22.5
100	10.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.24544556012309904
2	0.011718369761364571
3	0.0017184446391266257
4	0.0
5	0.0
6	2.1714550995499844E-4
7	0.0
8	0.0
9	0.0
10	7.862165015612013E-4
11	0.0
12	6.701559703783574E-4
13	0.0
14	0.002654416664794723
15	0.0
16	0.0
17	0.0
18	2.2463328616034322E-5
19	0.0012092758571631813
20	0.0
21	0.0011830686404444744
22	0.0
23	0.0
24	2.32121062365688E-4
25	0.0
26	3.48181593548532E-4
27	0.0026881116577187744
28	9.135086970520625E-4
29	2.620721671870671E-5
30	7.487776205344774E-6
31	0.001044544780645596
32	0.00291648883198179
33	0.0
34	17.48611766291529
35	0.003500535375998682
36	0.0039385702840113515
37	7.412898443291327E-4
38	6.214854250436163E-4
39	0.0
40	0.0
41	0.0
42	3.406938173431872E-4
43	4.118276912939626E-5
44	0.0
45	2.2837717426301562E-4
46	0.0011568614237257677
47	0.0
48	1.0108497877215445E-4
49	3.743888102672387E-6
50	0.002216381756782053
51	0.0015799207793277474
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.67102E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	39.3146858754193
#Duplication Level	Relative count
1	100.0
2	16.106939153031337
3	5.876955375953372
4	3.3143209495914907
5	2.185323027006394
6	1.4740102916455622
7	1.1781805368979719
8	0.9095113303530137
9	0.7832514846542903
10++	14.985281919149655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158140	0.5920584645566113	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82619	0.30931629115468995	No Hit
TGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48219	0.18052654042275984	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37150	0.1390854430142792	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	24739215	9.988469	13.00193	18
AAAAA	11771840	7.5736833	9.106115	47
TGTGT	6224195	3.90355	56.038948	4
GTTTG	4860365	3.0482137	55.622253	7
TGTTT	5794705	2.9159236	45.117126	6
GTGTT	4597365	2.8832715	55.581337	5
TTTGA	5195790	2.8698971	49.191525	8
CTTTG	4184010	2.8120332	59.452	1
TTGTG	3945895	2.474697	55.476864	3
GAGGG	2289830	2.4485743	16.770914	11
TTTGT	4662955	2.3464215	44.7441	2
GGGGG	1899405	2.3061602	6.99837	13
TTGAG	3165520	2.1791725	34.14373	9
GAGAG	2242500	2.1119356	5.1700087	11
TGAGG	2394815	2.0547085	20.637424	10
AGGGG	1615275	1.7272552	9.596168	12
ATTTT	3545270	1.571203	6.948163	12
TGAGC	1601050	1.47209	7.4525084	10
ACTTT	2367950	1.4016463	5.5554156	5
TGAGA	1842880	1.3925587	5.754972	10
AACTT	2130935	1.3845417	6.0861497	4
GAGTG	1456310	1.249488	7.416614	11
TTGAT	2257895	1.2471493	13.693929	9
TGAGT	1756440	1.2091491	9.53137	10
TGATT	2006235	1.1081449	9.435777	10
GATTT	1975120	1.0909585	8.1214905	11
TTGAC	1329475	0.98079425	9.066769	9
TAACT	1448835	0.94135785	5.7832828	3
GGTAA	1091600	0.8248595	6.4768896	1
GTAAC	981425	0.7947394	6.956533	2
>>END_MODULE