FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_6_CTTGTA_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_6_CTTGTA_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34987315
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 130702 0.3735696780390264 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 105109 0.30042030947501974 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77477 0.22144311445448156 No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45933 0.13128472419218223 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 25896910 8.208706 10.847254 18
AAAAA 9348725 4.6931715 5.23341 44
TGTGT 8438230 3.976429 38.500996 4
GTTTG 6666890 3.1417031 38.01176 7
TGTTT 7863035 3.0389569 31.71413 6
TTTGA 7162150 3.0346959 34.38765 8
GTGTT 6376395 3.00481 38.096695 5
GGGGG 3435885 2.9349663 5.386895 13
TTGTG 5813265 2.7394412 38.100952 3
CTTTG 5293455 2.6949158 41.41603 1
GAGGG 3060825 2.350893 10.868222 11
TTTGT 5877735 2.2716649 31.567007 2
TTGAG 4294870 2.2188573 23.33637 9
TGAGG 3097295 1.9510547 13.523661 10
AACTT 3574175 1.7936971 20.714954 2
ACTTT 3795695 1.7375096 18.418648 3
AGGGG 2177205 1.6722212 5.9590206 12
TGAGC 2179770 1.4834114 5.8502817 10
TAACT 2654495 1.3321565 20.478014 1
TTGAT 3059035 1.2961528 8.855274 9
TGAGT 2244985 1.1598258 6.389295 10
TGATT 2623410 1.1115729 5.6202784 10
TTGAC 1895360 1.057877 7.2690797 9
GTAAC 1244030 0.7612228 5.517026 1
Produced by FastQC (version 0.10.1)