##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_6_CTTGTA_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34987315	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15729875241927	34.0	31.0	34.0	31.0	34.0
2	32.33903230356488	34.0	31.0	34.0	31.0	34.0
3	32.41629990755221	34.0	31.0	34.0	31.0	34.0
4	35.7828211739026	37.0	37.0	37.0	35.0	37.0
5	35.80328227530464	37.0	37.0	37.0	35.0	37.0
6	35.79435915559682	37.0	37.0	37.0	35.0	37.0
7	35.8360222554946	37.0	37.0	37.0	35.0	37.0
8	35.81887446921834	37.0	37.0	37.0	35.0	37.0
9	37.5534879712833	39.0	38.0	39.0	35.0	39.0
10	37.529513225007406	39.0	38.0	39.0	35.0	39.0
11	37.51698459856094	39.0	38.0	39.0	35.0	39.0
12	37.478314097552214	39.0	38.0	39.0	35.0	39.0
13	37.48444243292176	39.0	38.0	39.0	35.0	39.0
14	38.954775780879444	41.0	39.0	41.0	36.0	41.0
15	38.89986399356452	41.0	39.0	41.0	36.0	41.0
16	38.87255243793358	41.0	39.0	41.0	36.0	41.0
17	38.83288080265662	41.0	39.0	41.0	36.0	41.0
18	38.79790395461898	41.0	39.0	41.0	36.0	41.0
19	38.79343945084097	41.0	39.0	41.0	35.0	41.0
20	38.752385114433615	41.0	39.0	41.0	35.0	41.0
21	38.71401009194332	41.0	39.0	41.0	35.0	41.0
22	38.66406967782466	41.0	39.0	41.0	35.0	41.0
23	38.623408112340144	41.0	39.0	41.0	35.0	41.0
24	38.5869683340948	41.0	39.0	41.0	35.0	41.0
25	38.53143817980888	41.0	39.0	41.0	35.0	41.0
26	38.47626086768876	41.0	39.0	41.0	34.0	41.0
27	38.414028055596724	40.0	39.0	41.0	34.0	41.0
28	38.343558544003734	40.0	38.0	41.0	34.0	41.0
29	38.27719732137204	40.0	38.0	41.0	34.0	41.0
30	38.216472370057545	40.0	38.0	41.0	34.0	41.0
31	38.16045769731115	40.0	38.0	41.0	34.0	41.0
32	38.10429294159898	40.0	38.0	41.0	34.0	41.0
33	38.01367584223025	40.0	38.0	41.0	33.0	41.0
34	37.98054231940919	40.0	38.0	41.0	33.0	41.0
35	37.91656584679333	40.0	38.0	41.0	33.0	41.0
36	37.84877153334001	40.0	38.0	41.0	33.0	41.0
37	37.79744958994424	40.0	38.0	41.0	33.0	41.0
38	37.72709580600855	40.0	38.0	41.0	33.0	41.0
39	37.657536224200115	40.0	38.0	41.0	33.0	41.0
40	37.573053548121656	40.0	37.0	41.0	32.0	41.0
41	37.50766187688309	40.0	37.0	41.0	32.0	41.0
42	37.410273837818075	40.0	37.0	41.0	32.0	41.0
43	37.349195987174205	40.0	37.0	41.0	32.0	41.0
44	37.26126354651679	40.0	37.0	41.0	31.0	41.0
45	37.207458903319676	40.0	37.0	41.0	31.0	41.0
46	37.11360345885359	40.0	36.0	41.0	31.0	41.0
47	37.014952076202476	40.0	36.0	41.0	31.0	41.0
48	37.24487517833249	40.0	37.0	41.0	32.0	41.0
49	37.27058844041048	40.0	36.0	41.0	32.0	41.0
50	37.241636090108656	40.0	36.0	41.0	32.0	41.0
51	35.731437693918494	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	135887.0
3	3330.0
4	2061.0
5	1780.0
6	1859.0
7	4517.0
8	14036.0
9	22297.0
10	25391.0
11	29879.0
12	38361.0
13	47611.0
14	54967.0
15	59338.0
16	60438.0
17	62121.0
18	63199.0
19	65756.0
20	69112.0
21	73375.0
22	79971.0
23	88693.0
24	100058.0
25	114871.0
26	133099.0
27	155984.0
28	184953.0
29	221415.0
30	270961.0
31	337659.0
32	434118.0
33	579577.0
34	837682.0
35	1280900.0
36	2018447.0
37	3046580.0
38	6259966.0
39	1.7997146E7
40	9920.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.037754305183892	25.204767683786034	30.91612173170541	23.841356279324668
2	22.01768750561301	27.71987044191833	31.664181437358323	18.59826061511033
3	23.55921828161676	26.636218777895586	29.950585119856733	19.853977820630913
4	21.776575678338965	24.00769789425176	32.07919361288011	22.13653281452917
5	28.261197896638397	23.88395241567668	28.194308404710593	19.660541282974332
6	20.97307507644645	23.463848201714406	35.26118750037862	20.301889221460527
7	25.778462179604244	23.789457171887065	30.885303613721838	19.546777034786853
8	23.397624922118666	24.09418282834696	32.7283109885037	19.779881261030678
9	23.67770591845575	24.533213350325187	31.997446171084725	19.791634560134337
10	23.351406519177658	26.74175136711412	30.096038060785457	19.810804052922762
11	27.13558558889605	24.141742061339425	28.4243246402667	20.298347709497822
12	22.119908055611575	28.894834479289187	28.61634029470717	20.36891717039207
13	24.773711424775392	24.585472298007037	29.625292676199688	21.015523601017883
14	25.844489554405737	25.027112322627037	27.856519330194267	21.271878792772952
15	24.339176990287076	27.05898711541199	27.22070841087976	21.381127483421178
16	25.050980675346963	25.54756025082185	27.777564587935593	21.623894485895597
17	24.514926164638624	25.672019202570233	28.110620242639605	21.702434390151538
18	24.381744779166336	25.591625796522273	28.26466726248772	21.761962161823675
19	23.971104141829226	25.833631076486157	28.60109244947858	21.594172332206043
20	23.469490884062164	25.928291551335654	28.767120181360333	21.83509738324185
21	23.669579637481995	26.104294429216026	28.464361138260973	21.761764795041007
22	23.17894363819086	26.30472345657226	28.868274590018135	21.648058315218744
23	23.14196281896513	26.46381002199549	28.62947204689248	21.764755112146904
24	22.9487312253813	26.53858504675666	28.570341983562297	21.94234174429975
25	22.940771970850914	26.81145092333927	28.414513497841575	21.833263607968238
26	22.94716915649219	26.73964110687358	28.358749183253106	21.95444055338112
27	22.956906977594375	26.763920148661104	28.277591062473622	22.001581811270896
28	23.004102272146845	26.842903293008863	28.182847819362287	21.970146615482005
29	23.07294533945525	26.798602002914794	28.0376468000131	22.090805857616857
30	23.159156865889102	26.709860873651763	27.973727308315837	22.157254952143294
31	23.199138605476378	26.651600644955025	27.872059786861236	22.27720096270736
32	23.29578119422278	26.755095378935167	27.732615075379652	22.216508351462394
33	23.25409715723098	26.64199227907614	27.715688366761537	22.38822219693135
34	23.32283310433745	26.714697919175123	27.64559416610147	22.316874810385954
35	23.421460732196035	26.583061725156014	27.567197817394053	22.4282797252539
36	23.350043031403093	26.585710577089884	27.63618264216059	22.428063749346432
37	23.29859856050874	26.52561869143254	27.685548870916556	22.490233877142163
38	23.265391097133413	26.620501681350444	27.7894920994213	22.324615122094844
39	23.209548538688193	26.448078714417818	27.791410627380948	22.550962119513045
40	23.282854329112748	26.534801844774293	27.776632315427836	22.405711510685126
41	23.26546949414179	26.550902357673866	27.760058109704715	22.423570038479625
42	23.1575741098904	26.474488180341265	27.75698208627606	22.610955623492274
43	23.258591456173104	26.574251953489032	27.622368642130162	22.544787948207702
44	23.298689352329983	26.491564020837426	27.632475100329362	22.577271526503228
45	23.285241366601948	26.508929646501013	27.50829689397298	22.697532092924064
46	23.161608066970643	26.72339879576376	27.361285796866508	22.75370734039909
47	23.259702111874407	26.556623375408083	27.473995578492694	22.70967893422482
48	23.17191272727941	26.41849403436766	27.377227667747096	23.03236557060583
49	23.270389804281532	26.577104107149058	27.375804255052984	22.77670183351643
50	23.15954832848108	26.548904619952868	27.493156933586327	22.79839011797973
51	23.250903150140395	26.51449189934216	27.407899118551178	22.82670583196626
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.87911058450856
2	40.615948120723345
3	43.413196102247674
4	43.91310849286813
5	47.92173917961273
6	41.274964297906976
7	45.3252392143911
8	43.17750618314935
9	43.469340478590084
10	43.16221057210042
11	47.43393329839387
12	42.48882522600364
13	45.789235025793275
14	47.116368347178685
15	45.720304473708254
16	46.674875161242554
17	46.21736055479016
18	46.14370694099001
19	45.565276474035265
20	45.30458826730401
21	45.431344432523005
22	44.8270019534096
23	44.906717931112034
24	44.89107296968105
25	44.77403557881915
26	44.901609709873306
27	44.95848878886527
28	44.974248887628846
29	45.1637511970721
30	45.3164118180324
31	45.47633956818374
32	45.51228954568518
33	45.64231935416233
34	45.6397079147234
35	45.84974045744993
36	45.778106780749525
37	45.7888324376509
38	45.59000621922826
39	45.760510658201234
40	45.688565839797874
41	45.689039532621415
42	45.76852973338267
43	45.8033794043808
44	45.87596087883322
45	45.98277345952601
46	45.91531540736973
47	45.96938104609922
48	46.20427829788524
49	46.04709163779796
50	45.95793844646081
51	46.07760898210666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189025.0
1	101505.0
2	13985.0
3	16802.5
4	19620.0
5	101678.0
6	183736.0
7	209442.5
8	235149.0
9	143564.5
10	51980.0
11	50735.5
12	49491.0
13	52585.0
14	55679.0
15	73227.0
16	90775.0
17	129098.0
18	167421.0
19	204375.0
20	241329.0
21	280956.5
22	320584.0
23	380656.5
24	440729.0
25	518966.5
26	698254.5
27	799305.0
28	872879.5
29	946454.0
30	1010327.0
31	1074200.0
32	1163022.5
33	1251845.0
34	1326681.5
35	1401518.0
36	1545510.5
37	1689503.0
38	1777990.0
39	1866477.0
40	1967714.0
41	2068951.0
42	2107158.0
43	2145365.0
44	2184913.5
45	2224462.0
46	2240158.5
47	2255855.0
48	2248536.5
49	2241218.0
50	2184931.5
51	2128645.0
52	2005256.0
53	1881867.0
54	1770849.0
55	1659831.0
56	1552122.0
57	1444413.0
58	1309565.0
59	1174717.0
60	1067230.5
61	959744.0
62	846010.0
63	732276.0
64	645765.5
65	559255.0
66	474965.0
67	390675.0
68	368536.0
69	346397.0
70	307907.5
71	269418.0
72	228588.5
73	187759.0
74	156524.5
75	109549.0
76	93808.0
77	86387.0
78	78966.0
79	82301.0
80	85636.0
81	75686.5
82	65737.0
83	59914.0
84	54091.0
85	47738.5
86	41386.0
87	46114.5
88	50843.0
89	35680.0
90	20517.0
91	13778.5
92	7040.0
93	5352.5
94	3665.0
95	2828.5
96	1992.0
97	1554.5
98	1117.0
99	743.5
100	370.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8465125146070797
2	0.450251755529111
3	0.4346118014486107
4	0.43539780060287564
5	0.439422116272712
6	0.44794520528368637
7	0.4438065624641388
8	0.4168567950984521
9	0.4069503475759715
10	0.4262916431283738
11	0.43939067630654144
12	0.4316993173097164
13	0.4036091366256599
14	0.40189994573747656
15	0.45056615519081705
16	0.41778570318985614
17	0.4260458397564946
18	0.40700751115082706
19	0.4214184483719314
20	0.42537130957319813
21	0.4340401657000544
22	0.4558623604011911
23	0.41361562040413785
24	0.4199436281406561
25	0.4032232824953844
26	0.40079668874276286
27	0.3963064899378532
28	0.39538615638267754
29	0.4264059702780851
30	0.4280780048426122
31	0.43626668694068127
32	0.4289068766780189
33	0.4236392532550726
34	0.4195949303340368
35	0.42889830214179053
36	0.43859324443730535
37	0.43033596604940966
38	0.4384760591088513
39	0.41910332359027835
40	0.4407854675330188
41	0.43591227277657635
42	0.44085978018033106
43	0.4002879329265478
44	0.42817232474112404
45	0.4214956191979865
46	0.4200808207203096
47	0.4283666808956332
48	0.4338115114006319
49	0.4765670072139003
50	0.42937847617057784
51	0.4044408666398093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4987315E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	42.91863220364775
#Duplication Level	Relative count
1	100.0
2	16.558763043643843
3	6.597608347932058
4	3.6232767156676062
5	2.3413816741564477
6	1.7305202224084089
7	1.327595399497296
8	1.0526315789473684
9	0.9680859166730139
10++	18.134663721532487
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	130702	0.3735696780390264	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105109	0.30042030947501974	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77477	0.22144311445448156	No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45933	0.13128472419218223	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	25896910	8.208706	10.847254	18
AAAAA	9348725	4.6931715	5.23341	44
TGTGT	8438230	3.976429	38.500996	4
GTTTG	6666890	3.1417031	38.01176	7
TGTTT	7863035	3.0389569	31.71413	6
TTTGA	7162150	3.0346959	34.38765	8
GTGTT	6376395	3.00481	38.096695	5
GGGGG	3435885	2.9349663	5.386895	13
TTGTG	5813265	2.7394412	38.100952	3
CTTTG	5293455	2.6949158	41.41603	1
GAGGG	3060825	2.350893	10.868222	11
TTTGT	5877735	2.2716649	31.567007	2
TTGAG	4294870	2.2188573	23.33637	9
TGAGG	3097295	1.9510547	13.523661	10
AACTT	3574175	1.7936971	20.714954	2
ACTTT	3795695	1.7375096	18.418648	3
AGGGG	2177205	1.6722212	5.9590206	12
TGAGC	2179770	1.4834114	5.8502817	10
TAACT	2654495	1.3321565	20.478014	1
TTGAT	3059035	1.2961528	8.855274	9
TGAGT	2244985	1.1598258	6.389295	10
TGATT	2623410	1.1115729	5.6202784	10
TTGAC	1895360	1.057877	7.2690797	9
GTAAC	1244030	0.7612228	5.517026	1
>>END_MODULE