FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_6_CTTGTA_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_6_CTTGTA_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34987315
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121477 0.34720297913686715 No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46633 0.13328544931212927 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 21521165 7.503721 9.599831 18
AAAAA 10082430 4.766026 5.4079633 46
TGTGT 6279975 3.1873515 41.665623 4
TGTTT 6073610 2.554985 34.684956 6
GTTTG 4866770 2.4700906 41.236374 7
TTTGA 5429420 2.4273417 36.584167 8
CTTTG 4500095 2.3900003 43.19886 1
GTGTT 4562670 2.315747 41.151363 5
TTTGT 5022435 2.1127875 34.46347 2
GAGGG 2681280 2.1052873 10.50386 11
TTGTG 3990580 2.0253873 41.1392 3
TTGAG 3290735 1.7750108 23.919945 9
TGAGG 2665425 1.7346231 13.810067 10
ATTTT 4593835 1.7022461 5.129668 12
AGGGG 1899025 1.4910764 5.7088513 12
TGAGC 2067825 1.4081752 5.835283 10
TTGAT 2649850 1.1846739 9.916866 9
TGAGT 2035885 1.0981492 6.5302787 10
GATTT 2402825 1.0742359 5.392791 11
TGATT 2402515 1.0740973 6.446577 10
TTGAC 1668475 0.94174224 7.554342 9
Produced by FastQC (version 0.10.1)