##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_6_CTTGTA_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34987315	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89759557142353	34.0	31.0	34.0	31.0	34.0
2	33.08902452217325	34.0	33.0	34.0	31.0	34.0
3	33.12859512083165	34.0	33.0	34.0	31.0	34.0
4	36.487873819411405	37.0	37.0	37.0	35.0	37.0
5	36.41404320394406	37.0	37.0	37.0	35.0	37.0
6	36.34022502155424	37.0	37.0	37.0	35.0	37.0
7	36.36812501902475	37.0	37.0	37.0	35.0	37.0
8	36.33827863041219	37.0	37.0	37.0	35.0	37.0
9	38.131818317581676	39.0	39.0	39.0	37.0	39.0
10	38.14171553318681	39.0	39.0	39.0	37.0	39.0
11	38.12274522923522	39.0	38.0	39.0	37.0	39.0
12	38.072404155620404	39.0	38.0	39.0	37.0	39.0
13	38.06031666048109	39.0	38.0	39.0	37.0	39.0
14	39.57280880227591	41.0	40.0	41.0	37.0	41.0
15	39.53908803805036	41.0	40.0	41.0	37.0	41.0
16	39.51689053589851	41.0	39.0	41.0	37.0	41.0
17	39.49878094389352	41.0	39.0	41.0	37.0	41.0
18	39.47128909434748	41.0	39.0	41.0	37.0	41.0
19	39.47107130112728	41.0	39.0	41.0	37.0	41.0
20	39.41548669853631	41.0	39.0	41.0	36.0	41.0
21	39.378030866329695	41.0	39.0	41.0	36.0	41.0
22	39.32777991109064	41.0	39.0	41.0	36.0	41.0
23	39.26033678205944	41.0	39.0	41.0	36.0	41.0
24	39.22622353272893	41.0	39.0	41.0	36.0	41.0
25	39.16491248328144	40.0	39.0	41.0	36.0	41.0
26	39.064985295384915	40.0	39.0	41.0	35.0	41.0
27	38.99748485986993	40.0	39.0	41.0	35.0	41.0
28	38.928126893990004	40.0	39.0	41.0	35.0	41.0
29	38.85561781462796	40.0	38.0	41.0	35.0	41.0
30	38.77129599684914	40.0	38.0	41.0	35.0	41.0
31	38.68222983101161	40.0	38.0	41.0	35.0	41.0
32	38.60209435905556	40.0	38.0	41.0	35.0	41.0
33	38.52525548187965	40.0	38.0	41.0	35.0	41.0
34	38.44320340100405	40.0	38.0	41.0	34.0	41.0
35	38.35945379060954	40.0	38.0	41.0	34.0	41.0
36	38.27872407471108	40.0	38.0	41.0	34.0	41.0
37	38.479365935911346	40.0	38.0	41.0	35.0	41.0
38	38.519898454625626	40.0	38.0	41.0	35.0	41.0
39	38.496334199980765	40.0	38.0	41.0	35.0	41.0
40	38.446361488442314	40.0	38.0	41.0	34.0	41.0
41	38.38759433240305	40.0	38.0	41.0	34.0	41.0
42	38.30328932071524	40.0	38.0	41.0	34.0	41.0
43	38.242660775769735	40.0	38.0	41.0	34.0	41.0
44	38.17078123885757	40.0	38.0	41.0	34.0	41.0
45	38.10320291797184	40.0	38.0	41.0	34.0	41.0
46	38.011842720711776	40.0	37.0	41.0	33.0	41.0
47	37.933943887949106	40.0	37.0	41.0	33.0	41.0
48	37.8582055524981	40.0	37.0	41.0	33.0	41.0
49	37.79028202078382	40.0	37.0	41.0	33.0	41.0
50	37.88479075916514	40.0	37.0	41.0	33.0	41.0
51	37.59521360813198	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	10.0
10	27.0
11	59.0
12	121.0
13	235.0
14	388.0
15	722.0
16	1158.0
17	1838.0
18	3063.0
19	5427.0
20	9541.0
21	15522.0
22	24180.0
23	36359.0
24	50932.0
25	70189.0
26	94670.0
27	123834.0
28	162199.0
29	208428.0
30	267272.0
31	342023.0
32	441869.0
33	589856.0
34	855161.0
35	1316764.0
36	2047090.0
37	3170947.0
38	6674132.0
39	1.8459717E7
40	13578.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.37230558395498	26.242775096343983	27.63456861986257	24.750350699838464
2	22.96088407147459	26.105865465410595	30.858414562213067	20.074835900901746
3	21.661976633531324	25.029317053909395	32.13113952871205	21.177566783847233
4	22.578731748921	24.9660426929017	31.402844145085158	21.052381413092146
5	26.82585081354428	25.262994166050873	27.470833827084235	20.440321193320614
6	22.178601015825308	24.840642958740904	31.573926150091825	21.406829875341963
7	26.794611872101743	25.33814564330738	27.148712628386534	20.71852985620434
8	22.57778569175714	25.514741557047177	30.866078177190793	21.041394574004894
9	21.705455248566516	25.984531822461943	31.270802003526132	21.03921092544541
10	22.3159793770971	25.486711398116718	31.095992933438875	21.10131629134731
11	26.559122927838274	25.29128342657903	26.949635889464513	21.19995775611818
12	22.236064699448928	29.982380757140128	26.51613020318936	21.265424340221593
13	24.514741985773984	25.81963777443339	27.840270109323907	21.825350130468717
14	23.778195048173657	26.065147534644982	28.15224677204548	22.00441064513588
15	23.996408412591823	26.102497433712763	27.858342373514517	22.042751780180904
16	23.956100529160565	26.06220182574243	27.764142195478296	22.217555449618708
17	23.753426063131737	26.29284070526704	27.697321157682435	22.256412073918792
18	23.689705826240168	26.140165371363878	27.944687953333947	22.225440849062007
19	23.316177116232613	26.372886099776917	28.278117422589794	22.032819361400676
20	22.978346304780906	26.4383272691935	28.390724009706435	22.192602416319158
21	23.151585081621725	26.48719685977618	28.071391017001446	22.28982704160065
22	22.977622689183857	26.59533681265158	28.233939307258627	22.193101190905935
23	23.06418811572929	26.665786537052288	28.054514454699824	22.215510892518598
24	22.860566488592347	26.67954799424942	28.08644746172994	22.373438055428295
25	22.91056123942524	26.90369143512989	27.90331661355885	22.28243071188603
26	22.897736633536358	26.8332465755445	27.823417240100778	22.44559955081836
27	22.95543393097216	26.809874525915077	27.747666032476438	22.48702551063632
28	22.976204375785912	26.905579922323277	27.746853395294835	22.371362306595977
29	23.06585972658948	26.910287342712635	27.6186383550724	22.40521457562548
30	23.062824340764646	26.781283445157193	27.679163148129543	22.476729065948618
31	23.164845878114892	26.731356440714073	27.4979749669645	22.605822714206536
32	23.253446184907084	26.82676146064718	27.393869886492855	22.52592246795288
33	23.111720559363512	26.691338231003847	27.409905616789537	22.787035592843104
34	23.12713050429849	26.822252579256222	27.365295107669734	22.68532180877555
35	23.196196149986758	26.787469499040363	27.35609756934167	22.66023678163121
36	23.28855505446856	26.70719188937618	27.345996350717755	22.658256705437505
37	23.338810464702142	26.652459222838417	27.317757907135025	22.690972405324413
38	23.21271866675108	26.73730750702076	27.459374919167136	22.59059890706103
39	23.107806357818543	26.62693893486825	27.42988137272037	22.835373334592838
40	23.18225686030085	26.716612198352806	27.42958776429651	22.67154317704983
41	23.200447345421424	26.623815118567173	27.47929120994486	22.696446326066543
42	23.088893352094804	26.70145490191629	27.374270331809193	22.83538141417972
43	23.181101493498428	26.78698836992779	27.271332481500792	22.76057765507299
44	23.256189371953504	26.586102036869708	27.332192462673753	22.825516128503036
45	23.281660463448883	26.53539572817461	27.213103011564243	22.969840796812267
46	23.11903900027767	26.788208812250954	27.211110655390392	22.881641532080984
47	23.130614624185938	26.83598612811529	27.166414456210774	22.866984791488
48	23.12345488643527	26.625464114636976	27.142988823234937	23.108092175692818
49	23.331944734827463	26.62821082440879	27.15728829148507	22.882556149278674
50	23.14548858636337	26.615883499491172	27.184295222425614	23.054332691719843
51	23.21252918422508	26.652772853300792	27.120426435173613	23.014271527300515
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	46.12265628379344
2	43.03571997237634
3	42.83954341737856
4	43.63111316201314
5	47.26617200686489
6	43.58543089116727
7	47.51314172830609
8	43.61918026576204
9	42.744666174011925
10	43.4172956684444
11	47.759080683956455
12	43.501489039670524
13	46.3400921162427
14	45.782605693309534
15	46.03916019277272
16	46.17365597877927
17	46.00983813705053
18	45.915146675302175
19	45.34899647763329
20	45.17094872110006
21	45.44141212322238
22	45.170723880089795
23	45.27969900824789
24	45.23400454402064
25	45.19299195131127
26	45.34333618435472
27	45.44245944160848
28	45.34756668238188
29	45.47107430221496
30	45.53955340671326
31	45.77066859232143
32	45.779368652859965
33	45.898756152206616
34	45.81245231307404
35	45.85643293161797
36	45.94681175990606
37	46.02978287002655
38	45.80331757381211
39	45.94317969241138
40	45.85380003735068
41	45.896893671487966
42	45.92427476627453
43	45.94167914857142
44	46.081705500456536
45	46.25150126026114
46	46.00068053235866
47	45.99759941567394
48	46.23154706212809
49	46.214500884106144
50	46.19982127808321
51	46.22680071152559
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83524.0
1	48769.5
2	14015.0
3	17529.5
4	21044.0
5	64541.0
6	108038.0
7	153181.0
8	198324.0
9	118103.5
10	37883.0
11	41462.5
12	45042.0
13	48685.5
14	52329.0
15	72223.0
16	92117.0
17	133973.5
18	175830.0
19	214729.5
20	253629.0
21	290363.0
22	327097.0
23	389399.5
24	451702.0
25	532553.5
26	716674.5
27	819944.0
28	882414.5
29	944885.0
30	1003184.0
31	1061483.0
32	1148923.5
33	1236364.0
34	1308649.5
35	1380935.0
36	1522853.0
37	1664771.0
38	1747352.5
39	1829934.0
40	1931235.0
41	2032536.0
42	2066679.5
43	2100823.0
44	2141284.5
45	2181746.0
46	2194222.0
47	2206698.0
48	2220992.5
49	2235287.0
50	2189587.5
51	2143888.0
52	2031103.5
53	1918319.0
54	1816534.5
55	1714750.0
56	1606603.0
57	1498456.0
58	1362326.0
59	1226196.0
60	1118700.0
61	1011204.0
62	891801.0
63	772398.0
64	683732.5
65	595067.0
66	505604.0
67	416141.0
68	396433.0
69	376725.0
70	333265.5
71	289806.0
72	246832.5
73	203859.0
74	168801.5
75	114849.0
76	95954.0
77	89101.0
78	82248.0
79	87127.0
80	92006.0
81	80923.0
82	69840.0
83	62856.0
84	55872.0
85	49310.5
86	42749.0
87	49159.5
88	55570.0
89	36544.5
90	17519.0
91	10705.0
92	3891.0
93	2616.5
94	1342.0
95	816.5
96	291.0
97	183.0
98	75.0
99	47.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22449564935177221
2	0.01631734244253953
3	0.0
4	0.0
5	1.0575261348291516E-4
6	0.0
7	2.8010151679258615E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0871956021775314E-4
15	0.0
16	4.0014502398940877E-5
17	0.0
18	0.0
19	0.004993238263639265
20	6.145084296980206E-4
21	0.0
22	2.858178742781491E-6
23	1.257598646823856E-4
24	6.802465407819948E-4
25	2.858178742781491E-6
26	0.0029239168538654652
27	0.0027638588442697016
28	0.0
29	0.0
30	0.0
31	0.004190090036917666
32	5.716357485562982E-6
33	0.0013662094390495527
34	0.0
35	0.007888573330076915
36	0.002026448728632077
37	6.28799323411928E-5
38	0.0
39	0.0
40	2.858178742781491E-6
41	0.0013747839752778972
42	2.2865429942251928E-5
43	0.0
44	2.858178742781491E-6
45	0.004884627471413569
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0014862529462463753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4987315E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	44.57098946952689
#Duplication Level	Relative count
1	100.0
2	17.377902383865923
3	6.966495763565111
4	3.8290555925587446
5	2.531792623801484
6	1.7913846249167429
7	1.4885608513143016
8	1.106739571554702
9	0.9580383834941681
10++	18.847101101318174
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121477	0.34720297913686715	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	46633	0.13328544931212927	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	21521165	7.503721	9.599831	18
AAAAA	10082430	4.766026	5.4079633	46
TGTGT	6279975	3.1873515	41.665623	4
TGTTT	6073610	2.554985	34.684956	6
GTTTG	4866770	2.4700906	41.236374	7
TTTGA	5429420	2.4273417	36.584167	8
CTTTG	4500095	2.3900003	43.19886	1
GTGTT	4562670	2.315747	41.151363	5
TTTGT	5022435	2.1127875	34.46347	2
GAGGG	2681280	2.1052873	10.50386	11
TTGTG	3990580	2.0253873	41.1392	3
TTGAG	3290735	1.7750108	23.919945	9
TGAGG	2665425	1.7346231	13.810067	10
ATTTT	4593835	1.7022461	5.129668	12
AGGGG	1899025	1.4910764	5.7088513	12
TGAGC	2067825	1.4081752	5.835283	10
TTGAT	2649850	1.1846739	9.916866	9
TGAGT	2035885	1.0981492	6.5302787	10
GATTT	2402825	1.0742359	5.392791	11
TGATT	2402515	1.0740973	6.446577	10
TTGAC	1668475	0.94174224	7.554342	9
>>END_MODULE