FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_5_CAGATC_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_5_CAGATC_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33488127
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 164740 0.49193554479771295 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92788 0.27707730563730837 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62179 0.18567476168493985 No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37725 0.11265186613751196 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 22175365 7.336558 9.645598 18
AAAAA 8322925 4.131906 4.533031 47
TGTGT 7560300 3.8035274 36.717663 4
GTTTG 5902330 2.9694157 36.23259 7
TGTTT 7107700 2.8997703 29.941141 6
GTGTT 5623275 2.8290255 36.316864 5
TTTGA 6375685 2.821058 32.084568 8
CTTTG 4849565 2.6145384 39.145973 1
TTGTG 5148740 2.5902905 36.30056 3
GGGGG 2630400 2.481466 5.005848 13
GAGGG 2765820 2.2948167 10.609906 11
TTTGT 5388990 2.1985784 29.77611 2
TTGAG 3900530 2.1282456 22.481382 9
TGAGG 2892895 1.9464525 13.251727 10
AACTT 3303360 1.6987767 17.897526 2
ACTTT 3496670 1.6579998 16.09672 3
AGGGG 1975730 1.6392745 5.821529 12
TGAGC 2032545 1.4655342 5.547743 10
TAACT 2417190 1.2430574 17.609692 1
TTGAT 2788535 1.2338468 8.2184105 9
TGAGT 2145845 1.1708372 6.2815332 10
TGATT 2468195 1.0921055 5.2113886 10
TTGAC 1736005 1.0150647 6.4994493 9
Produced by FastQC (version 0.10.1)