##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename 140701_JD_B_5_CAGATC_L004_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33488127 Filtered Sequences 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44785269716637 34.0 31.0 34.0 31.0 34.0 2 32.6009830290001 34.0 31.0 34.0 31.0 34.0 3 32.688581149969956 34.0 31.0 34.0 31.0 34.0 4 36.021004907201885 37.0 37.0 37.0 35.0 37.0 5 36.051619757653214 37.0 37.0 37.0 35.0 37.0 6 36.05083574844302 37.0 37.0 37.0 35.0 37.0 7 36.0773311986066 37.0 37.0 37.0 35.0 37.0 8 36.06746623960188 37.0 37.0 37.0 35.0 37.0 9 37.860572315674744 39.0 39.0 39.0 37.0 39.0 10 37.832299369863236 39.0 39.0 39.0 37.0 39.0 11 37.83406644390712 39.0 39.0 39.0 37.0 39.0 12 37.8022185295702 39.0 39.0 39.0 37.0 39.0 13 37.80311496668655 39.0 39.0 39.0 37.0 39.0 14 39.36135418979987 41.0 40.0 41.0 37.0 41.0 15 39.307926746694434 41.0 40.0 41.0 37.0 41.0 16 39.287345422453754 41.0 40.0 41.0 37.0 41.0 17 39.25319469195754 41.0 40.0 41.0 37.0 41.0 18 39.218578542777266 41.0 40.0 41.0 37.0 41.0 19 39.22255914760476 41.0 40.0 41.0 37.0 41.0 20 39.201145677690484 41.0 40.0 41.0 37.0 41.0 21 39.15949082491236 41.0 40.0 41.0 37.0 41.0 22 39.123318004616976 41.0 40.0 41.0 36.0 41.0 23 39.08444583956577 41.0 40.0 41.0 36.0 41.0 24 39.05256280830516 41.0 40.0 41.0 36.0 41.0 25 39.006518937293805 41.0 39.0 41.0 36.0 41.0 26 38.95800631071424 41.0 39.0 41.0 36.0 41.0 27 38.89511521501337 41.0 39.0 41.0 35.0 41.0 28 38.84620361120823 41.0 39.0 41.0 35.0 41.0 29 38.787434095672175 41.0 39.0 41.0 35.0 41.0 30 38.72633724782518 41.0 39.0 41.0 35.0 41.0 31 38.68829265966413 41.0 39.0 41.0 35.0 41.0 32 38.63355842505017 41.0 39.0 41.0 35.0 41.0 33 38.588936879031785 41.0 39.0 41.0 35.0 41.0 34 38.52636034257753 41.0 39.0 41.0 35.0 41.0 35 38.46975078659968 40.0 38.0 41.0 35.0 41.0 36 38.40501031903038 40.0 38.0 41.0 35.0 41.0 37 38.36486113421631 40.0 38.0 41.0 35.0 41.0 38 38.31888439147403 40.0 38.0 41.0 34.0 41.0 39 38.260236978914946 40.0 38.0 41.0 34.0 41.0 40 38.18745527332717 40.0 38.0 41.0 34.0 41.0 41 38.110831728510824 40.0 38.0 41.0 34.0 41.0 42 38.05196405878418 40.0 38.0 41.0 34.0 41.0 43 38.0011569473563 40.0 38.0 41.0 33.0 41.0 44 37.921008601048364 40.0 38.0 41.0 33.0 41.0 45 37.85968979393801 40.0 38.0 41.0 33.0 41.0 46 37.78586631614243 40.0 38.0 41.0 33.0 41.0 47 37.698904241494304 40.0 37.0 41.0 33.0 41.0 48 37.87546117464258 40.0 38.0 41.0 33.0 41.0 49 37.90487852605194 40.0 38.0 41.0 33.0 41.0 50 37.8760333774415 40.0 37.0 41.0 33.0 41.0 51 36.41163541335113 38.0 35.0 40.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 173410.0 3 3849.0 4 2261.0 5 1872.0 6 2426.0 7 4167.0 8 9911.0 9 14417.0 10 15620.0 11 17422.0 12 21342.0 13 27398.0 14 31993.0 15 34691.0 16 36892.0 17 37935.0 18 40301.0 19 41947.0 20 44385.0 21 47671.0 22 52117.0 23 57937.0 24 65333.0 25 75239.0 26 87940.0 27 104680.0 28 126423.0 29 153292.0 30 188787.0 31 235531.0 32 303275.0 33 410514.0 34 608387.0 35 971618.0 36 1599234.0 37 2496918.0 38 5373408.0 39 1.9949042E7 40 18542.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.960783461145315 25.72019995026718 30.581041099797535 23.73797548878997 2 21.99818199622532 28.00103575346365 31.207277083960623 18.793505166350407 3 23.19479719902298 26.824671961385583 29.97813551286248 20.002395326728962 4 21.774232897978273 24.44769294005598 31.72098013005853 22.05709403190722 5 27.580272893479453 24.393826803695504 28.253613040849125 19.772287261975922 6 21.124115597523975 24.029282718774343 34.41323872803919 20.4333629556625 7 25.559385840793414 24.354503296958267 30.397707825583186 19.688403036665136 8 23.10859661272893 24.64037179812705 32.294416924291866 19.95661466485216 9 23.20362081256338 24.965252733564704 31.855127269950103 19.975999183921818 10 23.07237937221832 26.79473678835721 30.159985030218895 19.972898809205574 11 26.609634296266915 24.629676788442598 28.397029709421606 20.363659205868878 12 21.917256551372706 29.092251400684066 28.55104928429898 20.43944276364425 13 24.448515609433194 25.0635345215567 29.509097180385503 20.9788526886246 14 25.300804907971923 25.487653827377148 28.009204728639226 21.2023365360117 15 24.02182094227883 27.23698704021122 27.40387837020903 21.337313647300927 16 24.593986491709867 25.907012107024567 27.93663307482098 21.56236832644459 17 24.189781924025684 26.000322611098447 28.1853371934564 21.624558271419467 18 24.05318392822714 25.944528376636523 28.329132320466822 21.673155374669523 19 23.646151886117735 26.186626152540093 28.64786955080599 21.519352410536182 20 23.20963549192887 26.239952807655914 28.764996040831132 21.785415659584082 21 23.376880366097126 26.38782400381875 28.530649436484147 21.70464619359998 22 22.99244513544788 26.55657359007372 28.840206242857857 21.610775031620534 23 22.956732960153126 26.696023619057208 28.647290169167743 21.69995325162192 24 22.785320842945904 26.77810506083384 28.587771060652646 21.848803035567613 25 22.78412517175343 27.00338770647003 28.443031603231674 21.769455518544866 26 22.791368096438404 26.934287332642366 28.416277143241697 21.858067427677533 27 22.78453328523152 26.928956693142187 28.33361910302675 21.952890918599547 28 22.809253876127567 26.99950218112169 28.2857840085827 21.90545993416804 29 22.8650877062729 26.973312644235502 28.159205017519863 22.002394631971736 30 22.923723950023465 26.90403176325868 28.108401675178975 22.06384261153888 31 22.971452281624167 26.847770566331935 28.023602373599665 22.157174778444233 32 23.033930992520965 26.943836747994236 27.902487810420386 22.119744449064417 33 22.9981340621411 26.853439131007374 27.866702190025915 22.281724616825617 34 23.06693013795293 26.899857580111565 27.809896935584216 22.223315346351292 35 23.122176188276978 26.824891400609413 27.76289716620008 22.290035244913533 36 23.085343113689795 26.837090704143957 27.778474253093826 22.299091929072425 37 23.09452034614411 26.771250159510924 27.801907017687626 22.332322476657335 38 23.033424159063348 26.862630727537685 27.879194919206963 22.224750194192012 39 23.008443212154177 26.700653336181208 27.877987884477 22.412915567187618 40 23.071526885443834 26.80064987576629 27.840276281306764 22.28754695748311 41 23.043497695638514 26.785909440387034 27.837028600830298 22.333564263144147 42 22.951555117343904 26.730537843655846 27.831245232926367 22.486661806073883 43 23.02885702948449 26.8617665082533 27.724779772424395 22.38459668983781 44 23.108194023643282 26.741951601443414 27.727531951401392 22.42232242351191 45 23.07194970254086 26.770929298208085 27.6358838601647 22.52123713908636 46 22.952636286797002 26.944198794615655 27.53520109567616 22.56796382291118 47 23.035834975335725 26.838759369384587 27.607161829396894 22.518243825882795 48 22.966554561013872 26.72247401500577 27.524895816798495 22.786075607181864 49 23.055153063887023 26.82525110467852 27.54048269697756 22.5791131344569 50 22.96722645693451 26.813504848843532 27.613776255995532 22.605492438226428 51 23.052042442900756 26.762875804337522 27.559629904363142 22.62545184839858 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.69875894993528 2 40.79168716257573 3 43.19719252575194 4 43.83132692988549 5 47.35256015545538 6 41.55747855318647 7 45.24778887745855 8 43.06521127758109 9 43.179619996485194 10 43.045278181423896 11 46.973293502135796 12 42.35669931501696 13 45.42736829805779 14 46.50314144398362 15 45.35913458957976 16 46.156354818154455 17 45.814340195445155 18 45.72633930289666 19 45.16550429665392 20 44.99505115151295 21 45.0815265596971 22 44.60322016706842 23 44.656686211775046 24 44.63412387851351 25 44.5535806902983 26 44.64943552411594 27 44.73742420383107 28 44.714713810295606 29 44.867482338244635 30 44.987566561562346 31 45.128627060068396 32 45.15367544158538 33 45.27985867896672 34 45.29024548430422 35 45.41221143319051 36 45.38443504276222 37 45.42684282280145 38 45.258174353255356 39 45.421358779341794 40 45.359073842926946 41 45.37706195878267 42 45.43821692341779 43 45.4134537193223 44 45.53051644715519 45 45.593186841627215 46 45.520600109708184 47 45.55407880121852 48 45.752630168195736 49 45.63426619834392 50 45.57271889516093 51 45.67749429129934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213115.0 1 113586.5 2 14058.0 3 16342.5 4 18627.0 5 84419.0 6 150211.0 7 174751.0 8 199291.0 9 122013.5 10 44736.0 11 43992.5 12 43249.0 13 47023.0 14 50797.0 15 67565.0 16 84333.0 17 119388.0 18 154443.0 19 191937.0 20 229431.0 21 269504.5 22 309578.0 23 369558.5 24 429539.0 25 505708.5 26 681553.0 27 781228.0 28 854366.5 29 927505.0 30 989327.0 31 1051149.0 32 1141022.5 33 1230896.0 34 1308690.5 35 1386485.0 36 1534123.5 37 1681762.0 38 1767175.0 39 1852588.0 40 1955069.5 41 2057551.0 42 2088499.0 43 2119447.0 44 2150677.0 45 2181907.0 46 2183078.0 47 2184249.0 48 2162213.5 49 2140178.0 50 2078258.5 51 2016339.0 52 1902532.0 53 1788725.0 54 1677452.5 55 1566180.0 56 1456142.5 57 1346105.0 58 1218965.5 59 1091826.0 60 987880.0 61 883934.0 62 772403.0 63 660872.0 64 576966.5 65 493061.0 66 416388.0 67 339715.0 68 316856.5 69 293998.0 70 260055.0 71 226112.0 72 191711.0 73 157310.0 74 131243.5 75 90391.5 76 75606.0 77 69289.5 78 62973.0 79 67997.5 80 73022.0 81 63818.5 82 54615.0 83 49383.0 84 44151.0 85 37571.5 86 30992.0 87 34779.5 88 38567.0 89 25837.0 90 13107.0 91 8606.0 92 4105.0 93 2934.5 94 1764.0 95 1369.0 96 974.0 97 727.5 98 481.0 99 333.0 100 185.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8321426874665161 2 0.5577081095040042 3 0.5422996634001059 4 0.550959449001134 5 0.5299609619851239 6 0.5473462281124293 7 0.5406781931996376 8 0.5405497894821051 9 0.5303163118080626 10 0.5804654288369129 11 0.551192367372472 12 0.5431805726250382 13 0.5347268301986552 14 0.518126917041374 15 0.5811193919564387 16 0.5343744665086824 17 0.5520493875336773 18 0.5359899644432189 19 0.5500098587179868 20 0.5507026415660691 21 0.5839114262795289 22 0.5914125922897987 23 0.563113010172232 24 0.5574453298030074 25 0.5402870097811083 26 0.5614915399717637 27 0.5735883646165102 28 0.551986678741394 29 0.6156988117012336 30 0.6178518135696272 31 0.5985942420727203 32 0.6268131986002083 33 0.6173531293643266 34 0.5949481737213909 35 0.6012220390826873 36 0.6254425635688733 37 0.5938552490558818 38 0.6055041537557475 39 0.5836874663070885 40 0.5877933991351622 41 0.5957723464199715 42 0.5890535472467601 43 0.5736809347384522 44 0.5866945022037213 45 0.5651794141846154 46 0.5803489696512438 47 0.5714950854074341 48 0.6155315882551449 49 0.6064955499004169 50 0.5910184227383036 51 0.5629398144602115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3.3488127E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 41.29607160441725 #Duplication Level Relative count 1 100.0 2 16.410678848242036 3 6.436514771408245 4 3.5144344179428613 5 2.249597483805744 6 1.5980828996143333 7 1.2303890365821695 8 0.933837570674355 9 0.8155165312464897 10++ 16.584416070693077 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 164740 0.49193554479771295 No Hit CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92788 0.27707730563730837 No Hit TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62179 0.18567476168493985 No Hit TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37725 0.11265186613751196 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 22175365 7.336558 9.645598 18 AAAAA 8322925 4.131906 4.533031 47 TGTGT 7560300 3.8035274 36.717663 4 GTTTG 5902330 2.9694157 36.23259 7 TGTTT 7107700 2.8997703 29.941141 6 GTGTT 5623275 2.8290255 36.316864 5 TTTGA 6375685 2.821058 32.084568 8 CTTTG 4849565 2.6145384 39.145973 1 TTGTG 5148740 2.5902905 36.30056 3 GGGGG 2630400 2.481466 5.005848 13 GAGGG 2765820 2.2948167 10.609906 11 TTTGT 5388990 2.1985784 29.77611 2 TTGAG 3900530 2.1282456 22.481382 9 TGAGG 2892895 1.9464525 13.251727 10 AACTT 3303360 1.6987767 17.897526 2 ACTTT 3496670 1.6579998 16.09672 3 AGGGG 1975730 1.6392745 5.821529 12 TGAGC 2032545 1.4655342 5.547743 10 TAACT 2417190 1.2430574 17.609692 1 TTGAT 2788535 1.2338468 8.2184105 9 TGAGT 2145845 1.1708372 6.2815332 10 TGATT 2468195 1.0921055 5.2113886 10 TTGAC 1736005 1.0150647 6.4994493 9 >>END_MODULE