FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_5_CAGATC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_5_CAGATC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33488127
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 87470 0.2611970505248024 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 18667285 6.7779045 8.6202965 18
AAAAA 8819665 4.218445 4.736848 47
TGTGT 5720940 3.1128109 38.8187 4
TGTTT 5575215 2.4780576 31.861938 6
GTTTG 4381500 2.3840098 38.326336 7
CTTTG 4118830 2.3382974 40.009205 1
TTTGA 4904620 2.3035219 33.3443 8
GTGTT 4096975 2.229198 38.286674 5
GAGGG 2422730 2.0873652 10.281846 11
TTTGT 4596630 2.0430987 31.570843 2
TTGTG 3613605 1.9661919 38.20891 3
TGAGG 2481885 1.7467858 13.357579 10
TTGAG 3012840 1.7322019 22.618782 9
AGGGG 1738130 1.4975306 5.681273 12
TGAGC 1902660 1.397204 5.470466 10
TTGAT 2441330 1.146604 8.986996 9
TGAGT 1936445 1.1133397 6.304639 10
TGATT 2276025 1.0689663 5.8213186 10
TTGAC 1536670 0.9218148 6.6244235 9
Produced by FastQC (version 0.10.1)