##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_5_CAGATC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	33488127	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09744608887801	34.0	33.0	34.0	31.0	34.0
2	33.28205518929142	34.0	34.0	34.0	31.0	34.0
3	33.301125500390036	34.0	34.0	34.0	31.0	34.0
4	36.593614566738836	37.0	37.0	37.0	35.0	37.0
5	36.54099645525114	37.0	37.0	37.0	35.0	37.0
6	36.506766204034044	37.0	37.0	37.0	35.0	37.0
7	36.52210065376305	37.0	37.0	37.0	35.0	37.0
8	36.50098824577439	37.0	37.0	37.0	35.0	37.0
9	38.339394765195436	39.0	39.0	39.0	37.0	39.0
10	38.31675378560288	39.0	39.0	39.0	37.0	39.0
11	38.307009317063326	39.0	39.0	39.0	37.0	39.0
12	38.27794982382861	39.0	39.0	39.0	37.0	39.0
13	38.276584205500654	39.0	39.0	39.0	37.0	39.0
14	39.828466250142924	41.0	40.0	41.0	38.0	41.0
15	39.81560354211509	41.0	40.0	41.0	38.0	41.0
16	39.804531199968274	41.0	40.0	41.0	38.0	41.0
17	39.76878641197222	41.0	40.0	41.0	38.0	41.0
18	39.725542996178916	41.0	40.0	41.0	38.0	41.0
19	39.74411641475201	41.0	40.0	41.0	38.0	41.0
20	39.71859781826556	41.0	40.0	41.0	38.0	41.0
21	39.68016336058449	41.0	40.0	41.0	37.0	41.0
22	39.616004382687635	41.0	40.0	41.0	37.0	41.0
23	39.593758259457154	41.0	40.0	41.0	37.0	41.0
24	39.57157374014975	41.0	40.0	41.0	37.0	41.0
25	39.52400712049378	41.0	40.0	41.0	37.0	41.0
26	39.42397423421143	41.0	39.0	41.0	37.0	41.0
27	39.33657236190008	41.0	39.0	41.0	36.0	41.0
28	39.28520343941601	41.0	39.0	41.0	36.0	41.0
29	39.2144923482881	41.0	39.0	41.0	36.0	41.0
30	39.15267414030053	41.0	39.0	41.0	36.0	41.0
31	39.11423938400616	40.0	39.0	41.0	35.0	41.0
32	39.05286285494557	40.0	39.0	41.0	35.0	41.0
33	38.98749682238126	40.0	39.0	41.0	35.0	41.0
34	32.469669892257635	40.0	35.0	41.0	2.0	41.0
35	38.84704707432577	40.0	38.0	41.0	35.0	41.0
36	38.783849243046646	40.0	38.0	41.0	35.0	41.0
37	38.92283121716542	40.0	39.0	41.0	35.0	41.0
38	38.93774220337853	40.0	39.0	41.0	35.0	41.0
39	38.91804913424988	40.0	39.0	41.0	35.0	41.0
40	38.88680507572131	40.0	38.0	41.0	35.0	41.0
41	38.84304909020442	40.0	38.0	41.0	35.0	41.0
42	38.775001898434034	40.0	38.0	41.0	35.0	41.0
43	38.713008255134724	40.0	38.0	41.0	35.0	41.0
44	38.64733238141387	40.0	38.0	41.0	35.0	41.0
45	38.58263616833512	40.0	38.0	41.0	35.0	41.0
46	38.491818876582734	40.0	38.0	41.0	35.0	41.0
47	38.42559555510525	40.0	38.0	41.0	35.0	41.0
48	38.37658621516814	40.0	38.0	41.0	34.0	41.0
49	38.317065508023184	40.0	38.0	41.0	34.0	41.0
50	38.392877421899406	40.0	38.0	41.0	35.0	41.0
51	38.121623045684224	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	12.0
11	36.0
12	78.0
13	172.0
14	312.0
15	531.0
16	820.0
17	1319.0
18	2171.0
19	3513.0
20	6200.0
21	10228.0
22	15889.0
23	23900.0
24	33854.0
25	47259.0
26	64353.0
27	85078.0
28	110851.0
29	144499.0
30	188301.0
31	241533.0
32	316134.0
33	432330.0
34	652524.0
35	1065365.0
36	1732767.0
37	2921553.0
38	7853792.0
39	1.7510464E7
40	22277.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.106850641062177	26.715028117266314	27.71580618555911	24.4623150561124
2	22.83430264680216	26.484408457964324	30.66451679569277	20.016772099540738
3	21.528937122818643	25.403184312922615	31.920435596071588	21.14744296818715
4	22.393826325371975	25.338535654741158	31.262614358814396	21.005023661072475
5	26.356633800391403	25.595558091379672	27.642638837340765	20.40516927088816
6	22.11590337102628	25.228524048771906	31.318875499801408	21.33669708040041
7	26.378895421651976	25.70795613621508	27.233876054041485	20.67927238809146
8	22.355579934345087	25.848826958880082	30.778436787462017	21.017156319312814
9	21.553432952520755	26.20560415337651	31.205800193005718	21.03516270109702
10	22.16446532749333	25.794471927195854	31.010142089346793	21.030920655964024
11	26.065539586612296	25.683290677916983	27.134637897186664	21.116531838284057
12	22.062344641711633	29.999787385980508	26.748811526451004	21.189056445856856
13	24.197456609024446	26.13276938420593	27.998403732761762	21.67137027400786
14	23.517124383672126	26.389127993165403	28.278456077416216	21.815291545746263
15	23.7238021702438	26.371215087663757	28.024350242102226	21.88063249999022
16	23.64504291326893	26.36112195823911	27.94865475755034	22.045180370941615
17	23.525612525298893	26.565513801354136	27.831816332994674	22.077057340352297
18	23.42628720667019	26.470597105868755	28.053143630210297	22.04997205725076
19	23.067388933006196	26.662707804787892	28.388028824519925	21.881874437685983
20	22.764674178403588	26.696148160212125	28.483695131710412	22.055482529673874
21	22.921213729037177	26.721918333211995	28.21111416961038	22.145753768140448
22	22.804368843918922	26.82867572737048	28.333845007217036	22.033110421493564
23	22.868376604042382	26.853627854433306	28.183621616102926	22.09437392542139
24	22.671623699047004	26.89676110404408	28.21934345708485	22.212271739824065
25	22.73546382573143	27.091709249669293	28.04594595571141	22.126880968887868
26	22.719259006653722	27.04907008536187	27.992243196223605	22.239427711760804
27	22.777727443565578	27.02566354012572	27.88328784242038	22.313321173888326
28	22.793450285133094	27.0627692952702	27.93174961560708	22.212030803989624
29	22.83523572467426	27.119140626458073	27.8229156821756	22.222707966692063
30	22.839236978458395	27.01067399296539	27.835429061359186	22.314659967217036
31	22.935869428762782	26.932948484767767	27.70353648749258	22.42764559897687
32	22.974625761084873	27.04149530906273	27.626664623300744	22.357214306551654
33	22.88081982011117	26.925862410877745	27.613867446214595	22.579450322796493
34	22.89100099241733	27.027644960094865	27.584685506769745	22.496668540718062
35	22.94747978570998	27.03503280710452	27.524474157906386	22.493013249279105
36	23.055053891406242	26.95761823608584	27.49578125808619	22.491546614421726
37	23.04224488904339	26.97333237497749	27.497417052823454	22.487005683155672
38	22.979741325881413	26.988574121055624	27.596172196624774	22.435512356438185
39	22.904192282835048	26.851035891018928	27.60660815697456	22.638163669171465
40	22.962132220771856	26.946260685167612	27.60949276141959	22.48211433264094
41	22.988290745552895	26.88379376965454	27.608390878355188	22.51952460643738
42	22.893196087054882	26.93346433339871	27.539227157744513	22.6341124218019
43	22.968272797017974	27.0190510613499	27.436008030074436	22.57666811155769
44	23.02178321289811	26.846293911869125	27.499388066701968	22.632534808530796
45	23.043631086207917	26.812787943934545	27.416459585476893	22.72712138438064
46	22.9304167367919	27.007453003352754	27.39168951186507	22.670440747990277
47	22.9236469391077	27.031452072550966	27.38209574993549	22.662805238405838
48	22.90773252129548	26.87819736769731	27.36883144200443	22.84523866900278
49	23.067575561929754	26.921523559678327	27.364313328123725	22.646587550268187
50	22.9240866260663	26.882765985275647	27.394427393244857	22.798719995413197
51	22.997202242148045	26.896432791549568	27.335814256829334	22.77055070947305
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.569165697174576
2	42.851074746342896
3	42.6763800910058
4	43.398849986444446
5	46.76180307127956
6	43.45260045142669
7	47.05816780974344
8	43.3727362536579
9	42.58859565361777
10	43.195385983457356
11	47.18207142489636
12	43.25140108756849
13	45.868826883032305
14	45.33241592941839
15	45.60443467023402
16	45.69022328421055
17	45.602669865651194
18	45.47625926392095
19	44.94926337069218
20	44.82015670807746
21	45.06696749717762
22	44.83747926541248
23	44.96275052946377
24	44.88389543887107
25	44.8623447946193
26	44.958686718414526
27	45.0910486174539
28	45.00548108912272
29	45.057943691366326
30	45.15389694567543
31	45.36351502773965
32	45.33184006763653
33	45.46027014290767
34	45.38766953313539
35	45.44049303498909
36	45.54660050582797
37	45.52925057219906
38	45.4152536823196
39	45.54235595200652
40	45.444246553412796
41	45.507815351990274
42	45.52730850885678
43	45.54494090857567
44	45.65431802142891
45	45.77075247058856
46	45.600857484782175
47	45.58645217751354
48	45.75297119029826
49	45.714163112197944
50	45.72280662147949
51	45.76775295162109
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66735.0
1	39264.5
2	11794.0
3	15528.5
4	19263.0
5	54742.0
6	90221.0
7	129891.5
8	169562.0
9	101389.5
10	33217.0
11	36690.0
12	40163.0
13	44895.5
14	49628.0
15	69123.5
16	88619.0
17	128129.5
18	167640.0
19	206778.5
20	245917.0
21	285350.0
22	324783.0
23	386803.5
24	448824.0
25	533566.5
26	716674.5
27	815040.0
28	881534.0
29	948028.0
30	1006170.5
31	1064313.0
32	1150482.0
33	1236651.0
34	1318672.0
35	1400693.0
36	1540729.0
37	1680765.0
38	1766577.5
39	1852390.0
40	1942442.0
41	2032494.0
42	2063558.0
43	2094622.0
44	2124730.5
45	2154839.0
46	2155815.5
47	2156792.0
48	2142607.5
49	2128423.0
50	2069087.0
51	2009751.0
52	1910869.0
53	1811987.0
54	1702267.0
55	1592547.0
56	1479788.5
57	1367030.0
58	1239476.0
59	1111922.0
60	1006469.5
61	901017.0
62	788360.5
63	675704.0
64	589301.0
65	502898.0
66	427489.0
67	352080.0
68	330018.5
69	307957.0
70	270203.0
71	232449.0
72	197405.5
73	162362.0
74	135314.5
75	92700.0
76	77133.0
77	73572.5
78	70012.0
79	73238.0
80	76464.0
81	66684.0
82	56904.0
83	50814.0
84	44724.0
85	39930.5
86	35137.0
87	36611.5
88	38086.0
89	24517.5
90	10949.0
91	6581.0
92	2213.0
93	1426.0
94	639.0
95	382.5
96	126.0
97	79.5
98	33.0
99	22.0
100	11.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.245815479617597
2	0.011786266816295818
3	0.0017588323168984637
4	0.0
5	0.0
6	2.269461054062534E-4
7	0.0
8	0.0
9	0.0
10	7.584777733314258E-4
11	0.0
12	6.360463217306839E-4
13	0.0
14	0.002621824744035401
15	0.0
16	0.0
17	0.0
18	3.583359559046106E-5
19	0.001158619590758241
20	0.0
21	0.0011616057237241128
22	0.0
23	0.0
24	2.388906372697404E-4
25	0.0
26	3.195162273482778E-4
27	0.002547171419888607
28	7.674361722290411E-4
29	3.583359559046106E-5
30	8.958398897615265E-6
31	9.645209479765769E-4
32	0.0026755751374210926
33	0.0
34	17.482587186796085
35	0.003502733968967569
36	0.0037266939414079504
37	6.509769865600426E-4
38	5.97226593174351E-4
39	0.0
40	0.0
41	0.0
42	3.0757169548479077E-4
43	5.673652635156335E-5
44	0.0
45	1.6125118015707476E-4
46	0.0012511897127002655
47	0.0
48	9.854238787376793E-5
49	0.0
50	0.0022306413255062008
51	0.001782721380625438
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.3488127E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	40.37715912464853
#Duplication Level	Relative count
1	100.0
2	15.561723286928885
3	6.103124794036015
4	3.276383528739757
5	2.068061485284102
6	1.5202888255842053
7	1.1614537944973746
8	0.8948906285471575
9	0.7689322094717802
10++	15.10841944153552
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87470	0.2611970505248024	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	18667285	6.7779045	8.6202965	18
AAAAA	8819665	4.218445	4.736848	47
TGTGT	5720940	3.1128109	38.8187	4
TGTTT	5575215	2.4780576	31.861938	6
GTTTG	4381500	2.3840098	38.326336	7
CTTTG	4118830	2.3382974	40.009205	1
TTTGA	4904620	2.3035219	33.3443	8
GTGTT	4096975	2.229198	38.286674	5
GAGGG	2422730	2.0873652	10.281846	11
TTTGT	4596630	2.0430987	31.570843	2
TTGTG	3613605	1.9661919	38.20891	3
TGAGG	2481885	1.7467858	13.357579	10
TTGAG	3012840	1.7322019	22.618782	9
AGGGG	1738130	1.4975306	5.681273	12
TGAGC	1902660	1.397204	5.470466	10
TTGAT	2441330	1.146604	8.986996	9
TGAGT	1936445	1.1133397	6.304639	10
TGATT	2276025	1.0689663	5.8213186	10
TTGAC	1536670	0.9218148	6.6244235	9
>>END_MODULE