FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_4_GCCAAT_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_4_GCCAAT_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33178573
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 123845 0.3732680124609338 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71964 0.21689902094342633 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58037 0.17492313487985153 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 19549765 5.695712 7.5324206 18
TGTGT 8967985 4.365072 40.205296 4
AAAAA 8315560 3.7042527 4.0979886 44
GTTTG 6827755 3.3233373 39.64782 7
GTGTT 6484015 3.1560256 39.72527 5
TGTTT 8159520 3.0726714 31.227747 6
TTTGA 7233200 2.9652581 33.570854 8
TTGTG 5959220 2.9005873 39.71788 3
CTTTG 5263275 2.8131402 43.80117 1
GGGGG 2629415 2.7636986 6.597734 13
GAGGG 2855360 2.5277135 13.539582 11
TTGAG 4604190 2.4396656 26.14075 9
TTTGT 6043225 2.2757275 31.061382 2
TGAGG 3156915 2.1621454 15.985214 10
AGGGG 2030220 1.7972566 7.4692287 12
AACTT 3647010 1.7872622 21.167805 2
ACTTT 3747450 1.6869674 18.936028 3
TGAGC 2103470 1.5819658 6.564042 10
TAACT 2717725 1.3318545 20.90886 1
TGAGT 2416910 1.2806708 7.4027505 10
GAGTG 1860815 1.274457 6.039048 11
TTGAT 2858455 1.1718267 7.7170186 9
TTGAC 1803625 1.0494508 6.630919 9
GTAAC 1246735 0.7897151 6.0766883 1
Produced by FastQC (version 0.10.1)