##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_4_GCCAAT_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	33178573	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1885370416624	34.0	31.0	34.0	31.0	34.0
2	32.376209971417396	34.0	31.0	34.0	31.0	34.0
3	32.45475334337013	34.0	31.0	34.0	31.0	34.0
4	35.82148921835788	37.0	37.0	37.0	35.0	37.0
5	35.84374918716365	37.0	37.0	37.0	35.0	37.0
6	35.83563379895814	37.0	37.0	37.0	35.0	37.0
7	35.882148608380476	37.0	37.0	37.0	35.0	37.0
8	35.867818848025806	37.0	37.0	37.0	35.0	37.0
9	37.60941337049065	39.0	38.0	39.0	35.0	39.0
10	37.58882472130432	39.0	38.0	39.0	35.0	39.0
11	37.577901436568716	39.0	38.0	39.0	35.0	39.0
12	37.538396452433325	39.0	38.0	39.0	35.0	39.0
13	37.547633859961365	39.0	38.0	39.0	35.0	39.0
14	39.018782513642165	41.0	39.0	41.0	36.0	41.0
15	38.969174683914225	41.0	39.0	41.0	36.0	41.0
16	38.94247456031337	41.0	39.0	41.0	36.0	41.0
17	38.91215197832649	41.0	39.0	41.0	36.0	41.0
18	38.882242403855045	41.0	39.0	41.0	36.0	41.0
19	38.886884616767574	41.0	39.0	41.0	36.0	41.0
20	38.856832631108034	41.0	39.0	41.0	36.0	41.0
21	38.82216664351417	41.0	39.0	41.0	36.0	41.0
22	38.78197018901325	41.0	39.0	41.0	35.0	41.0
23	38.7498053638413	41.0	39.0	41.0	35.0	41.0
24	38.726164142140775	41.0	39.0	41.0	35.0	41.0
25	38.681069616827706	41.0	39.0	41.0	35.0	41.0
26	38.637351431600145	41.0	39.0	41.0	35.0	41.0
27	38.58466764076924	41.0	39.0	41.0	35.0	41.0
28	38.52468890087587	40.0	39.0	41.0	35.0	41.0
29	38.468900395444976	40.0	39.0	41.0	34.0	41.0
30	38.417704161055994	40.0	38.0	41.0	34.0	41.0
31	38.37001124189398	40.0	38.0	41.0	34.0	41.0
32	38.32169201490372	40.0	38.0	41.0	34.0	41.0
33	38.241663949802785	40.0	38.0	41.0	34.0	41.0
34	38.2159231501608	40.0	38.0	41.0	34.0	41.0
35	38.16215362848788	40.0	38.0	41.0	34.0	41.0
36	38.10796193675961	40.0	38.0	41.0	34.0	41.0
37	38.067715058149126	40.0	38.0	41.0	33.0	41.0
38	38.00985235260118	40.0	38.0	41.0	33.0	41.0
39	37.95312055765629	40.0	38.0	41.0	33.0	41.0
40	37.8830934350311	40.0	38.0	41.0	33.0	41.0
41	37.83150803381447	40.0	38.0	41.0	33.0	41.0
42	37.75047950374478	40.0	38.0	41.0	33.0	41.0
43	37.70304542633585	40.0	38.0	41.0	33.0	41.0
44	37.62896650196499	40.0	38.0	41.0	33.0	41.0
45	37.58812665029325	40.0	38.0	41.0	32.0	41.0
46	37.50925155822705	40.0	37.0	41.0	32.0	41.0
47	37.427972324186456	40.0	37.0	41.0	32.0	41.0
48	37.64878163988548	40.0	38.0	41.0	33.0	41.0
49	37.68157259204608	40.0	38.0	41.0	33.0	41.0
50	37.668237992031784	40.0	37.0	41.0	33.0	41.0
51	36.17114452149585	38.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	128669.0
3	3135.0
4	1845.0
5	1640.0
6	1658.0
7	4169.0
8	11295.0
9	17722.0
10	20610.0
11	24717.0
12	31242.0
13	39010.0
14	44893.0
15	48163.0
16	49732.0
17	51098.0
18	52161.0
19	53726.0
20	57284.0
21	60913.0
22	66946.0
23	74801.0
24	85026.0
25	98038.0
26	114315.0
27	134432.0
28	160891.0
29	192471.0
30	235608.0
31	294471.0
32	380194.0
33	507618.0
34	726052.0
35	1100743.0
36	1709566.0
37	2688546.0
38	5843927.0
39	1.8050836E7
40	10410.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.85376327737568	25.887572071493864	32.151940434363816	23.10672421676664
2	21.0452333566142	28.571337744860482	32.66028626172772	17.723142636797604
3	22.92372161396055	27.60724047104781	30.742439397593458	18.726598517398184
4	20.91969231686683	24.74284640953351	33.01875440730615	21.318706866293514
5	27.833943891014023	24.5383794329029	28.884502898439855	18.74317377764322
6	20.030446325401364	24.100707020435333	36.642300665138045	19.22654598902526
7	25.004884657006254	24.381619376264265	32.11562835337682	18.49786761335265
8	22.74357042549934	24.59828334433847	33.93412578975848	18.72402044040371
9	23.057739327758018	25.204023460360148	33.014762077489785	18.723475134392046
10	22.739383819827445	27.632477641381104	30.89708374736782	18.73105479142363
11	26.860641019415798	24.740015501835288	29.182277583113486	19.217065895635425
12	21.36549332341766	29.828147028110074	29.44008450924046	19.366275139231806
13	24.38020731814451	25.23273366853119	30.431084485165528	19.95597452815877
14	25.45940055327064	25.725335529566472	28.51272021340646	20.302543703756427
15	23.932708991930678	28.01779678176599	27.69146109064205	20.358033135661284
16	24.61386935958227	26.368985797133277	28.37070660100316	20.64643824228129
17	24.13508328676266	26.55322784767406	28.639396145053258	20.67229272051002
18	23.85089962857004	26.458571873217807	28.89149484859769	20.799033649614458
19	23.374922440381248	26.802458299408883	29.21692176845011	20.605697491759763
20	22.9276300028098	26.84013561491495	29.340005537266993	20.892228845008255
21	23.028384310936318	27.07381850900546	29.16990411345539	20.72789306660283
22	22.531639107921155	27.307458567416752	29.490102249865206	20.670800074796887
23	22.47492216964158	27.433766036612973	29.306618714381035	20.78469307936442
24	22.254673241940406	27.54880312033428	29.273815186938002	20.922708450787308
25	22.343978036503	27.705952344818407	29.13139898788353	20.81867063079507
26	22.282585522961398	27.65287230011723	29.15878985407933	20.905752322842037
27	22.312373955556104	27.675593317241482	29.015382191823974	20.996650535378443
28	22.306548037815745	27.744623590026627	28.998084884201912	20.95074348795571
29	22.338761053343145	27.703029173189154	28.889848602634288	21.068361170833416
30	22.373564093759672	27.68952582100735	28.77775193468071	21.15915815055228
31	22.443682948683797	27.578327440486284	28.763313394522072	21.214676216307847
32	22.460464069383992	27.677424654893766	28.622928309244628	21.23918296647761
33	22.445120954415604	27.60759175630168	28.589318510958766	21.357968778323944
34	22.507050065704163	27.66253498852277	28.51505623693487	21.315358708838193
35	22.55196354378442	27.58288811847901	28.464886792013637	21.400261545722934
36	22.470725443750013	27.647100528119	28.497096156794218	21.385077871336776
37	22.56681905622601	27.533915334075303	28.50794747996633	21.39131812973236
38	22.45196064479531	27.611394159576125	28.532269114292962	21.4043760813356
39	22.426304078613178	27.482772676815912	28.55299504636144	21.537928198209467
40	22.48473776547197	27.566912088869174	28.537898472276535	21.410451673382326
41	22.466699044949628	27.55136995009579	28.44564444286226	21.536286562092318
42	22.341639309900195	27.473020054121093	28.526794797475162	21.65854583850355
43	22.386077843541266	27.62734585667325	28.43701043665457	21.549565863130912
44	22.430362842101694	27.58937262992721	28.34579816060558	21.63446636736552
45	22.46482318787415	27.592552656824104	28.302177128265804	21.640447027035943
46	22.306085844812984	27.803470499963378	28.186631686441917	21.70381196878172
47	22.44818635637137	27.650271424438834	28.18933666591973	21.712205553270074
48	22.332774313561114	27.612481060357247	28.21198996004444	21.842754666037198
49	22.393421624150857	27.699698283564757	28.175002134221856	21.731877958062533
50	22.38388542301503	27.653951260660786	28.227603816141322	21.734559500182858
51	22.421028434720416	27.590737431086616	28.23872251748856	21.749511616704407
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.96048749414232
2	38.7683759934118
3	41.65032013135873
4	42.23839918316034
5	46.577117668657245
6	39.256992314426626
7	43.502752270358904
8	41.46759086590305
9	41.78121446215007
10	41.470438611251076
11	46.07770691505122
12	40.73176846264946
13	44.33618184630328
14	45.76194425702707
15	44.29074212759196
16	45.260307601863566
17	44.80737600727268
18	44.6499332781845
19	43.98061993214101
20	43.81985884781805
21	43.75627737753915
22	43.202439182718045
23	43.259615249006
24	43.17738169272772
25	43.16264866729807
26	43.18833784580343
27	43.30902449093455
28	43.25729152577146
29	43.40712222417656
30	43.53272224431195
31	43.658359164991644
32	43.6996470358616
33	43.80308973273955
34	43.82240877454236
35	43.95222508950735
36	43.85580331508678
37	43.95813718595837
38	43.85633672613091
39	43.96423227682264
40	43.895189438854295
41	44.00298560704195
42	44.000185148403745
43	43.93564370667218
44	44.06482920946721
45	44.10527021491009
46	44.009897813594705
47	44.16039190964144
48	44.17552897959831
49	44.12529958221339
50	44.11844492319789
51	44.17054005142482
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	161625.0
1	85786.0
2	9947.0
3	11889.0
4	13831.0
5	67160.5
6	120490.0
7	139258.5
8	158027.0
9	98531.5
10	39036.0
11	40003.0
12	40970.0
13	46163.5
14	51357.0
15	70777.5
16	90198.0
17	132610.0
18	175022.0
19	216751.0
20	258480.0
21	303652.0
22	348824.0
23	421191.5
24	493559.0
25	578431.5
26	778301.0
27	893298.0
28	974519.0
29	1055740.0
30	1123589.0
31	1191438.0
32	1287193.5
33	1382949.0
34	1466811.5
35	1550674.0
36	1708583.0
37	1866492.0
38	1953111.0
39	2039730.0
40	2130511.5
41	2221293.0
42	2241752.5
43	2262212.0
44	2263949.0
45	2265686.0
46	2225087.0
47	2184488.0
48	2131606.0
49	2078724.0
50	1995257.5
51	1911791.0
52	1784332.5
53	1656874.0
54	1529985.5
55	1403097.0
56	1277170.5
57	1151244.0
58	1025865.5
59	900487.0
60	796130.0
61	691773.0
62	594874.0
63	497975.0
64	427834.5
65	357694.0
66	299572.5
67	241451.0
68	217140.5
69	192830.0
70	169270.5
71	145711.0
72	122422.5
73	99134.0
74	83356.5
75	57549.5
76	47520.0
77	43863.0
78	40206.0
79	40170.0
80	40134.0
81	35124.5
82	30115.0
83	27841.0
84	25567.0
85	22357.5
86	19148.0
87	21821.5
88	24495.0
89	17010.0
90	9525.0
91	6420.5
92	3316.0
93	2533.0
94	1750.0
95	1387.0
96	1024.0
97	764.5
98	505.0
99	369.5
100	234.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8423207351322796
2	0.4478492791115519
3	0.4326286124481604
4	0.4338281818208396
5	0.43736661007090333
6	0.4460288270987423
7	0.44176402643959406
8	0.41573216545509656
9	0.405620217602487
10	0.4245691941000597
11	0.43722495238116477
12	0.42997328426391335
13	0.4027056859859524
14	0.40056273667948283
15	0.44842495185070197
16	0.4165369016925472
17	0.4248826494135236
18	0.4058583230809836
19	0.4203707012956826
20	0.42380966776358947
21	0.4318479881578994
22	0.4534884607605034
23	0.41243184268352956
24	0.41905358618045446
25	0.4025549863160178
26	0.4000774837422935
27	0.3954419618951062
28	0.39472161747281903
29	0.4248042855851576
30	0.4267724232745031
31	0.4345364702695321
32	0.42735111000705184
33	0.42211580347352495
34	0.4183724236723503
35	0.427691691261104
36	0.43735756809070725
37	0.42855670736652834
38	0.4363418523153482
39	0.41779072294640285
40	0.43873496307390913
41	0.43427123885044727
42	0.438873606770249
43	0.39948372704275137
44	0.4268206471688822
45	0.4201747917247677
46	0.4188034247283631
47	0.4266910454527384
48	0.43210719159018685
49	0.47370934247232394
50	0.4275379775977707
51	0.40336575054026585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.3178573E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.595738172461424
#Duplication Level	Relative count
1	100.0
2	12.727661022831173
3	3.926881519497594
4	2.002148973231268
5	1.2113001288049172
6	0.8649283564359078
7	0.619331415719539
8	0.49986986031673997
9	0.440472774110879
10++	11.184004164469865
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	123845	0.3732680124609338	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71964	0.21689902094342633	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58037	0.17492313487985153	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	19549765	5.695712	7.5324206	18
TGTGT	8967985	4.365072	40.205296	4
AAAAA	8315560	3.7042527	4.0979886	44
GTTTG	6827755	3.3233373	39.64782	7
GTGTT	6484015	3.1560256	39.72527	5
TGTTT	8159520	3.0726714	31.227747	6
TTTGA	7233200	2.9652581	33.570854	8
TTGTG	5959220	2.9005873	39.71788	3
CTTTG	5263275	2.8131402	43.80117	1
GGGGG	2629415	2.7636986	6.597734	13
GAGGG	2855360	2.5277135	13.539582	11
TTGAG	4604190	2.4396656	26.14075	9
TTTGT	6043225	2.2757275	31.061382	2
TGAGG	3156915	2.1621454	15.985214	10
AGGGG	2030220	1.7972566	7.4692287	12
AACTT	3647010	1.7872622	21.167805	2
ACTTT	3747450	1.6869674	18.936028	3
TGAGC	2103470	1.5819658	6.564042	10
TAACT	2717725	1.3318545	20.90886	1
TGAGT	2416910	1.2806708	7.4027505	10
GAGTG	1860815	1.274457	6.039048	11
TTGAT	2858455	1.1718267	7.7170186	9
TTGAC	1803625	1.0494508	6.630919	9
GTAAC	1246735	0.7897151	6.0766883	1
>>END_MODULE