FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_4_GCCAAT_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_4_GCCAAT_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 33178573
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81263 0.24492614555785747 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 15858740 5.0921187 6.4895306 18
AAAAA 8950625 3.6832616 4.058872 47
TGTGT 6629865 3.517048 43.122173 4
GTTTG 4845930 2.5706959 42.562416 7
TGTTT 6191280 2.5552425 33.665844 6
CTTTG 4369630 2.446783 44.877274 1
GTGTT 4485725 2.3796124 42.426605 5
TTTGA 5326150 2.3100162 35.01415 8
GAGGG 2415925 2.225106 13.060331 11
GGGGG 1874800 2.1120014 5.5030594 13
TTGTG 3979895 2.1112769 42.444675 3
TTTGT 5081235 2.0971088 33.390835 2
TTGAG 3398855 1.8947685 26.591604 9
TGAGG 2627555 1.8827696 16.240572 10
AGGGG 1731925 1.5951309 7.131492 12
TGAGC 1937895 1.4657259 6.4378104 10
GAGTG 1688710 1.210042 5.98336 11
TGAGT 2160065 1.2041768 7.61618 10
TTGAT 2461750 1.067691 8.31154 9
TTGAC 1597215 0.93986183 6.9103503 9
Produced by FastQC (version 0.10.1)