##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename 140701_JD_B_4_GCCAAT_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33178573 Filtered Sequences 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.892019195641716 34.0 31.0 34.0 31.0 34.0 2 33.086907806432784 34.0 33.0 34.0 31.0 34.0 3 33.124449565688074 34.0 33.0 34.0 31.0 34.0 4 36.48654410182138 37.0 37.0 37.0 35.0 37.0 5 36.410420333629176 37.0 37.0 37.0 35.0 37.0 6 36.33664570203185 37.0 37.0 37.0 35.0 37.0 7 36.367484400248316 37.0 37.0 37.0 35.0 37.0 8 36.339138756811515 37.0 37.0 37.0 35.0 37.0 9 38.13093103793222 39.0 39.0 39.0 37.0 39.0 10 38.14288088881942 39.0 39.0 39.0 37.0 39.0 11 38.122776407532655 39.0 38.0 39.0 37.0 39.0 12 38.075183070712534 39.0 38.0 39.0 37.0 39.0 13 38.06476960296032 39.0 38.0 39.0 37.0 39.0 14 39.57343011708189 41.0 40.0 41.0 37.0 41.0 15 39.543693123872444 41.0 40.0 41.0 37.0 41.0 16 39.52035785264182 41.0 39.0 41.0 37.0 41.0 17 39.51306483253514 41.0 39.0 41.0 37.0 41.0 18 39.48548573803943 41.0 39.0 41.0 37.0 41.0 19 39.49420296044679 41.0 39.0 41.0 37.0 41.0 20 39.450024146608115 41.0 39.0 41.0 37.0 41.0 21 39.4183024086057 41.0 39.0 41.0 37.0 41.0 22 39.38068475699663 41.0 39.0 41.0 36.0 41.0 23 39.32171311285751 41.0 39.0 41.0 36.0 41.0 24 39.296437101137535 41.0 39.0 41.0 36.0 41.0 25 39.24481803361465 41.0 39.0 41.0 36.0 41.0 26 39.159508457461385 40.0 39.0 41.0 36.0 41.0 27 39.10214501991994 40.0 39.0 41.0 36.0 41.0 28 39.042897083005954 40.0 39.0 41.0 36.0 41.0 29 38.97725577287486 40.0 39.0 41.0 35.0 41.0 30 38.90380014233885 40.0 38.0 41.0 35.0 41.0 31 38.82434205352955 40.0 38.0 41.0 35.0 41.0 32 38.75618155729603 40.0 38.0 41.0 35.0 41.0 33 38.6900740125261 40.0 38.0 41.0 35.0 41.0 34 38.61818782863266 40.0 38.0 41.0 35.0 41.0 35 38.545196353080044 40.0 38.0 41.0 35.0 41.0 36 38.47749090956986 40.0 38.0 41.0 34.0 41.0 37 38.66883548608314 40.0 38.0 41.0 35.0 41.0 38 38.7145413698172 40.0 38.0 41.0 35.0 41.0 39 38.70114178810523 40.0 38.0 41.0 35.0 41.0 40 38.662801561718766 40.0 38.0 41.0 35.0 41.0 41 38.615779376647694 40.0 38.0 41.0 35.0 41.0 42 38.5475923874122 40.0 38.0 41.0 35.0 41.0 43 38.49788419170409 40.0 38.0 41.0 35.0 41.0 44 38.43915002010484 40.0 38.0 41.0 34.0 41.0 45 38.38223581827947 40.0 38.0 41.0 34.0 41.0 46 38.30552456249399 40.0 38.0 41.0 34.0 41.0 47 38.24438694213883 40.0 38.0 41.0 34.0 41.0 48 38.18151754145665 40.0 38.0 41.0 34.0 41.0 49 38.12711342347364 40.0 38.0 41.0 34.0 41.0 50 38.23376098785201 40.0 38.0 41.0 34.0 41.0 51 37.962301724067515 40.0 37.0 41.0 33.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 9.0 10 24.0 11 64.0 12 103.0 13 223.0 14 460.0 15 744.0 16 1114.0 17 1641.0 18 2710.0 19 4411.0 20 7337.0 21 11947.0 22 18818.0 23 28919.0 24 41434.0 25 57891.0 26 78686.0 27 104726.0 28 137556.0 29 180107.0 30 232371.0 31 301723.0 32 390585.0 33 523348.0 34 742392.0 35 1126473.0 36 1730913.0 37 2806596.0 38 6266360.0 39 1.8365269E7 40 13616.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 20.116840721665163 27.364587945952657 28.5734701655669 23.945101166815284 2 22.104853789212086 27.016738386070298 31.668257868869443 19.21014995584817 3 20.73851699408531 26.054842684162455 33.131669044355824 20.074971277396408 4 21.780566029768668 26.02610727109933 32.19394034818797 19.999386350944025 5 26.053130815979962 26.279370543408458 28.132928039020904 19.534570601590673 6 21.284447646377075 25.819603513387996 32.47538705175777 20.42056178847716 7 25.948228539631707 26.239483655561845 28.034047859887945 19.778239944918504 8 21.74668874396738 26.314600691235274 31.868971579941068 20.06973898485628 9 20.77686101810346 26.96861917479091 32.19648114462307 20.05803866248256 10 21.550761089092045 26.408522753525293 31.978035945066114 20.062680212316543 11 25.90439016168658 26.22119703580983 27.71517629766657 20.159236504837022 12 21.44198003934648 31.07374750565674 27.218177828202556 20.266094626794224 13 23.9401887477198 26.724561059331876 28.536588960592123 20.798661232356196 14 23.077104380879494 26.99023450521441 28.902939775490427 21.02972133841567 15 23.354301584941584 27.043824338075055 28.537125451417094 21.064748625566267 16 23.251015324231634 27.037472146172448 28.453971063967685 21.25754146562823 17 23.15870245534671 27.303889772474545 28.277816529360685 21.25959124281807 18 22.965753318135572 27.184222997903586 28.62169294085351 21.22833074310733 19 22.513331257190995 27.462447082182077 28.959786376768715 21.06443528385821 20 22.204364204225318 27.477618402878935 29.04479586612653 21.273221526769216 21 22.367571384097804 27.517964681603395 28.82609508251003 21.288368851788775 22 22.213689606653357 27.61048606914125 28.91236850097105 21.263455823234345 23 22.279832755746114 27.692150639906117 28.745961357731876 21.28205524661589 24 22.074048832246547 27.7610543414462 28.773769812710842 21.39112701359641 25 22.153352470986395 27.874557108726684 28.656827062961 21.31526335732593 26 22.12182632823813 27.835236493265125 28.621412900974185 21.421524277522558 27 22.196319245477696 27.827742130290467 28.49595393333115 21.47998469090069 28 22.16418710955411 27.87377865829251 28.55620704362421 21.405827188529177 29 22.21934921673696 27.88662731215113 28.445900913218903 21.44812255789301 30 22.200614233770693 27.82950309526573 28.447278308202108 21.52260436276147 31 22.29790362308619 27.76032605484965 28.341534738875207 21.600235583188958 32 22.314788662839035 27.84878528975826 28.26539756639911 21.571028481003598 33 22.262787461791444 27.785800304146363 28.235608995751864 21.715803238310325 34 22.318373969850963 27.82049728299044 28.187963358158896 21.673165388999703 35 22.27971537558358 27.80811486496038 28.1723186607785 21.739851098677534 36 22.339842408274137 27.815342292474103 28.152976844454574 21.69183845479718 37 22.436551782006642 27.769985260357355 28.13528751947945 21.658175438156555 38 22.312584088532077 27.817820856852403 28.188599310766016 21.6809957438495 39 22.257922304253412 27.667763167511755 28.265199952993758 21.809114575241075 40 22.29373825974186 27.804587249867176 28.18026345437652 21.721411036014448 41 22.303236148871537 27.7216974602139 28.187507612323536 21.78755877859103 42 22.240225813255464 27.721282468928887 28.155831086852878 21.88266063096277 43 22.267919720356872 27.835763159554812 28.084800392108484 21.811516727979832 44 22.311505751362656 27.71135237526196 28.089014801480904 21.888127071894477 45 22.356094323346277 27.693979613550855 28.068577150273654 21.881348912829214 46 22.225154168022836 27.882498141194922 28.00148758658186 21.89086010420038 47 22.316255132491683 27.884508474791847 27.922957988578958 21.876278404137516 48 22.222462671917807 27.811524624642537 27.964156264345668 22.001856439093988 49 22.32034813552711 27.798199759826918 27.965410085599522 21.91604201904645 50 22.265170958377265 27.74375196907956 27.98446756585945 22.006609506683727 51 22.32582634531703 27.783623834619387 27.943135638019633 21.94741418204395 >>END_MODULE >>Per base GC content pass #Base %GC 1 44.06194188848045 2 41.31500374506026 3 40.81348827148172 4 41.7799523807127 5 45.587701417570635 6 41.70500943485423 7 45.72646848455021 8 41.816427728823655 9 40.83489968058602 10 41.61344130140859 11 46.063626666523604 12 41.7080746661407 13 44.738849980076 14 44.10682571929516 15 44.41905021050785 16 44.50855678985986 17 44.41829369816478 18 44.1940840612429 19 43.57776654104921 20 43.477585730994534 21 43.655940235886575 22 43.4771454298877 23 43.56188800236201 24 43.46517584584296 25 43.46861582831232 26 43.543350605760686 27 43.67630393637839 28 43.57001429808328 29 43.667471774629966 30 43.72321859653216 31 43.89813920627515 32 43.88581714384263 33 43.97859070010177 34 43.991539358850666 35 44.01956647426111 36 44.031680863071315 37 44.09472722016319 38 43.99357983238158 39 44.06703687949449 40 44.01514929575631 41 44.09079492746256 42 44.12288644421823 43 44.0794364483367 44 44.199632823257126 45 44.23744323617549 46 44.11601427222322 47 44.1925335366292 48 44.224319111011795 49 44.236390154573556 50 44.27178046506099 51 44.27324052736098 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 55855.0 1 32597.5 2 9340.0 3 11519.0 4 13698.0 5 36805.5 6 59913.0 7 93829.5 8 127746.0 9 78339.0 10 28932.0 11 33766.0 12 38600.0 13 43967.5 14 49335.0 15 71687.0 16 94039.0 17 142083.5 18 190128.0 19 233458.5 20 276789.0 21 317091.5 22 357394.0 23 429255.5 24 501117.0 25 593605.5 26 804711.5 27 923329.0 28 993413.5 29 1063498.0 30 1124880.0 31 1186262.0 32 1279016.5 33 1371771.0 34 1453483.0 35 1535195.0 36 1692003.5 37 1848812.0 38 1929366.5 39 2009921.0 40 2091449.0 41 2172977.0 42 2192412.5 43 2211848.0 44 2222920.0 45 2233992.0 46 2194633.5 47 2155275.0 48 2119944.5 49 2084614.0 50 2004230.0 51 1923846.0 52 1811718.0 53 1699590.0 54 1574016.5 55 1448443.0 56 1321208.5 57 1193974.0 58 1067564.0 59 941154.0 60 834837.5 61 728521.0 62 626541.5 63 524562.0 64 451828.5 65 379095.0 66 316932.5 67 254770.0 68 232005.0 69 209240.0 70 183486.0 71 157732.0 72 132494.5 73 107257.0 74 89956.5 75 60352.0 76 48048.0 77 45011.0 78 41974.0 79 42777.5 80 43581.0 81 38108.5 82 32636.0 83 29549.0 84 26462.0 85 23428.0 86 20394.0 87 23796.5 88 27199.0 89 17741.0 90 8283.0 91 5033.0 92 1783.0 93 1229.5 94 676.0 95 420.5 96 165.0 97 102.0 98 39.0 99 29.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22360817024891338 2 0.01670355141554762 3 0.0 4 0.0 5 1.506996699345689E-4 6 0.0 7 3.013993398691378E-4 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.646932012416568E-4 15 0.0 16 2.7125940588222406E-5 17 0.0 18 3.013993398691378E-6 19 0.004994187061631614 20 5.997846863395842E-4 21 0.0 22 3.013993398691378E-6 23 1.0247577555550686E-4 24 7.565123430715359E-4 25 3.013993398691378E-6 26 0.0031104411874495028 27 0.0027698599333973767 28 0.0 29 0.0 30 0.0 31 0.004017653200455607 32 6.027986797382756E-6 33 0.0014105489105875652 34 0.0 35 0.00796297055934262 36 0.00207664145169836 37 6.329386137251894E-5 38 0.0 39 0.0 40 3.013993398691378E-6 41 0.0013984929369927996 42 2.1097953790839648E-5 43 0.0 44 3.013993398691378E-6 45 0.00492185122006302 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0013984929369927996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3.3178573E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 33.398844858013796 #Duplication Level Relative count 1 100.0 2 12.82545418046773 3 4.045635518083576 4 2.0188029040784237 5 1.2673558834353775 6 0.9026732242631068 7 0.6831944862658503 8 0.509886580347285 9 0.41219177623875003 10++ 11.16330422563485 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81263 0.24492614555785747 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 15858740 5.0921187 6.4895306 18 AAAAA 8950625 3.6832616 4.058872 47 TGTGT 6629865 3.517048 43.122173 4 GTTTG 4845930 2.5706959 42.562416 7 TGTTT 6191280 2.5552425 33.665844 6 CTTTG 4369630 2.446783 44.877274 1 GTGTT 4485725 2.3796124 42.426605 5 TTTGA 5326150 2.3100162 35.01415 8 GAGGG 2415925 2.225106 13.060331 11 GGGGG 1874800 2.1120014 5.5030594 13 TTGTG 3979895 2.1112769 42.444675 3 TTTGT 5081235 2.0971088 33.390835 2 TTGAG 3398855 1.8947685 26.591604 9 TGAGG 2627555 1.8827696 16.240572 10 AGGGG 1731925 1.5951309 7.131492 12 TGAGC 1937895 1.4657259 6.4378104 10 GAGTG 1688710 1.210042 5.98336 11 TGAGT 2160065 1.2041768 7.61618 10 TTGAT 2461750 1.067691 8.31154 9 TTGAC 1597215 0.93986183 6.9103503 9 >>END_MODULE