FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_2_TGACCA_L008_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_2_TGACCA_L008_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34423689
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 127577 0.3706081588176096 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43863 0.12742097455040335 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 14737090 4.7675242 5.9237895 18
TGTGT 6247820 3.2903473 25.00153 4
AAAAA 8450720 3.2882915 3.4176984 10
TGTTT 5912480 2.440433 19.746906 6
GTTTG 4408670 2.3217788 24.291994 7
CTTTG 3988365 2.2041898 25.743752 1
GTGTT 4099205 2.158802 24.335585 5
TTTGA 4952845 2.121246 20.007803 8
GAGGG 2376130 2.1137545 7.797227 11
TTGTG 3875265 2.0408666 24.331833 3
TTTGT 4711695 1.9447974 19.4836 2
TGAGG 2579180 1.7982495 9.345353 10
TTGAG 3150710 1.721713 14.920225 9
ACTTT 3151145 1.4162682 9.035406 3
AACTT 3032955 1.4144323 9.675819 2
TTGAT 2442455 1.0460751 5.133277 9
TAACT 2175120 1.014377 9.377543 1
Produced by FastQC (version 0.10.1)