##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_2_TGACCA_L008_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34423689	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.266469465256904	34.0	31.0	34.0	31.0	34.0
2	32.44402312605136	34.0	31.0	34.0	31.0	34.0
3	32.521763835363494	34.0	31.0	34.0	31.0	34.0
4	35.91252282694048	37.0	37.0	37.0	35.0	37.0
5	35.91883868111869	37.0	37.0	37.0	35.0	37.0
6	35.92810814087938	37.0	37.0	37.0	35.0	37.0
7	35.94675829194251	37.0	37.0	37.0	35.0	37.0
8	35.94001485430571	37.0	37.0	37.0	35.0	37.0
9	37.70154201079379	39.0	38.0	39.0	35.0	39.0
10	37.67303879023541	39.0	38.0	39.0	35.0	39.0
11	37.66248887503021	39.0	38.0	39.0	35.0	39.0
12	37.630029396326464	39.0	38.0	39.0	35.0	39.0
13	37.62535133872491	39.0	38.0	39.0	35.0	39.0
14	39.12695411581252	41.0	40.0	41.0	36.0	41.0
15	39.08345906796915	41.0	39.0	41.0	36.0	41.0
16	39.06086221613262	41.0	39.0	41.0	36.0	41.0
17	39.03252547976482	41.0	39.0	41.0	36.0	41.0
18	39.00779585244336	41.0	39.0	41.0	36.0	41.0
19	39.012970573839425	41.0	39.0	41.0	36.0	41.0
20	38.98132797446549	41.0	39.0	41.0	36.0	41.0
21	38.94361281267676	41.0	39.0	41.0	36.0	41.0
22	38.90356082986922	41.0	39.0	41.0	36.0	41.0
23	38.8685871813448	41.0	39.0	41.0	36.0	41.0
24	38.84602428868097	41.0	39.0	41.0	36.0	41.0
25	38.80313893725916	41.0	39.0	41.0	35.0	41.0
26	38.76096852954952	41.0	39.0	41.0	35.0	41.0
27	38.71229971895226	41.0	39.0	41.0	35.0	41.0
28	38.65553837068421	41.0	39.0	41.0	35.0	41.0
29	38.60155801430811	41.0	39.0	41.0	35.0	41.0
30	38.55314940243621	40.0	39.0	41.0	35.0	41.0
31	38.50875671692246	40.0	39.0	41.0	35.0	41.0
32	38.461756815197816	40.0	38.0	41.0	34.0	41.0
33	38.38462731289491	40.0	38.0	41.0	34.0	41.0
34	38.3600123449872	40.0	38.0	41.0	34.0	41.0
35	38.30834583126753	40.0	38.0	41.0	34.0	41.0
36	38.25571477827376	40.0	38.0	41.0	34.0	41.0
37	38.21619792114669	40.0	38.0	41.0	34.0	41.0
38	38.15999049375562	40.0	38.0	41.0	34.0	41.0
39	38.104572028872326	40.0	38.0	41.0	33.0	41.0
40	38.03830818364644	40.0	38.0	41.0	33.0	41.0
41	37.98866071559036	40.0	38.0	41.0	33.0	41.0
42	37.9085579119658	40.0	38.0	41.0	33.0	41.0
43	37.86471275638122	40.0	38.0	41.0	33.0	41.0
44	37.79132486352639	40.0	38.0	41.0	33.0	41.0
45	37.75190947722076	40.0	38.0	41.0	33.0	41.0
46	37.67807895313021	40.0	38.0	41.0	33.0	41.0
47	37.59764175768611	40.0	37.0	41.0	32.0	41.0
48	37.81936610570703	40.0	38.0	41.0	33.0	41.0
49	37.853497892105636	40.0	38.0	41.0	33.0	41.0
50	37.84282024509343	40.0	38.0	41.0	33.0	41.0
51	36.34350687981175	38.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132680.0
3	3366.0
4	1965.0
5	1717.0
6	1529.0
7	2842.0
8	6869.0
9	11434.0
10	15261.0
11	20458.0
12	28055.0
13	36672.0
14	42946.0
15	46553.0
16	48063.0
17	49422.0
18	50880.0
19	52847.0
20	55659.0
21	59811.0
22	65126.0
23	72940.0
24	83119.0
25	95278.0
26	110457.0
27	130234.0
28	154947.0
29	186055.0
30	227980.0
31	285892.0
32	371344.0
33	497613.0
34	715278.0
35	1071082.0
36	1655261.0
37	2685677.0
38	5934578.0
39	1.9399628E7
40	12171.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.986011244383718	28.183407972940305	29.113070764769322	22.71751001790665
2	22.097473708365573	29.374832724615313	29.87831923094117	18.649374336077944
3	22.16091927865492	27.90470993039123	29.667098320692247	20.267272470261606
4	21.63837831390712	26.50320726853271	30.399646992727686	21.45876742483249
5	25.04969375540306	26.62681965713315	28.393865322052324	19.92962126541147
6	21.47068205695221	26.26536477266772	31.581446849158727	20.682506321221343
7	24.008657025683817	26.642964392579476	29.255236160954055	20.09314242078265
8	22.000633059109816	26.62523166150829	30.93343781054777	20.44069746883412
9	21.997306604995458	26.619409507081766	30.999645876104587	20.38363801181819
10	22.122356543723505	27.658916761327312	29.92045646924653	20.29827022570266
11	24.27246516946283	26.569438817720247	28.67960019776244	20.478495815054483
12	21.426463958237402	29.262041994421235	28.750620440891794	20.56087360644957
13	23.013828361945123	26.87208178221267	29.2965477293081	20.817542126534114
14	23.420705194073808	27.095863157022514	28.51021264635155	20.973219002552124
15	22.755113400350137	28.00372867838952	28.15972446648282	21.081433454777518
16	23.0580040796981	27.28685491028141	28.406438231265813	21.248702778754673
17	22.924569890322765	27.314713721298855	28.489944685420593	21.270771702957788
18	22.802781469196358	27.266134571538238	28.567122998631074	21.363960960634333
19	22.58299689295134	27.43137613888847	28.730485845232874	21.255141122927316
20	22.380671542672676	27.470841051523863	28.74332435350989	21.405163052293567
21	22.455190571797083	27.51912918475351	28.62255982253849	21.403120420910916
22	22.29163719295395	27.607889546765502	28.760606451112857	21.33986680916769
23	22.269965758367512	27.671388722395186	28.659550407493743	21.39909511174356
24	22.176450154439888	27.71249968348895	28.590413816754513	21.520636345316646
25	22.218394276756158	27.8163853559386	28.494466423209726	21.470753944095513
26	22.21352531028381	27.78990896047176	28.498917439110233	21.497648290134194
27	22.223046280420835	27.770757728707522	28.40690765051322	21.59928834035842
28	22.236620913960085	27.8292916460974	28.374921828995745	21.559165610946774
29	22.2320896235829	27.83467505972442	28.345010406573596	21.588224910119077
30	22.28151457979539	27.79625133069387	28.256364356162965	21.665869733347765
31	22.340643670182082	27.760019877126403	28.198922513168075	21.700413939523447
32	22.367823636307357	27.809340418397916	28.138365837113984	21.684470108180744
33	22.345128806378955	27.738727272143826	28.105517756221964	21.810626165255258
34	22.372360239605253	27.780555200315142	28.07352741439536	21.77355714568425
35	22.40870225410556	27.75454334235352	28.03007061139321	21.80668379214771
36	22.40195282073977	27.738484291047154	28.051820719464693	21.80774216874838
37	22.432739946074093	27.717283422597184	28.020918607588953	21.82905802373977
38	22.405082857981977	27.760346007430837	28.063771822479964	21.77079931210722
39	22.380716108680783	27.645250573745102	28.07422080918559	21.899812508388525
40	22.421013665869065	27.68407911417511	28.05528332188945	21.839623898066378
41	22.400218003239704	27.718128696602047	28.032684147383264	21.848969152774988
42	22.345048309249222	27.679627134409827	28.020983901585332	21.954340654755615
43	22.409949966791913	27.739865583366303	27.938562879015816	21.91162157082597
44	22.434572678265322	27.70168701673772	27.956938365491645	21.906801939505314
45	22.439509326572303	27.68010123343978	27.913870128288025	21.96651931169989
46	22.36119976409595	27.79370525220231	27.864597820323368	21.980497163378374
47	22.402386706310516	27.73731842009481	27.884926760053535	21.975368113541144
48	22.360610587246757	27.669238983288206	27.874848286807953	22.09530214265708
49	22.405862772768838	27.75042002269134	27.84136109038682	22.002356114153006
50	22.3583414262085	27.717864050433256	27.896178622533128	22.02761590082511
51	22.395130701129116	27.695987964128637	27.876527784579324	22.032353550162924
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.703521262290366
2	40.74684804444352
3	42.428191748916525
4	43.097145738739606
5	44.979315020814525
6	42.15318837817355
7	44.101799446466465
8	42.44133052794394
9	42.38094461681365
10	42.420626769426164
11	44.75096098451731
12	41.987337564686975
13	43.83137048847923
14	44.393924196625925
15	43.83654685512766
16	44.30670685845277
17	44.19534159328055
18	44.1667424298307
19	43.838138015878656
20	43.785834594966246
21	43.858310992708
22	43.63150400212164
23	43.66906087011107
24	43.69708649975653
25	43.68914822085168
26	43.711173600418
27	43.82233462077926
28	43.79578652490686
29	43.82031453370198
30	43.947384313143154
31	44.041057609705526
32	44.052293744488104
33	44.15575497163422
34	44.1459173852895
35	44.21538604625327
36	44.20969498948815
37	44.26179796981386
38	44.175882170089196
39	44.28052861706931
40	44.26063756393544
41	44.2491871560147
42	44.29938896400484
43	44.321571537617885
44	44.34137461777064
45	44.4060286382722
46	44.341696927474324
47	44.37775481985166
48	44.45591272990384
49	44.408218886921844
50	44.38595732703361
51	44.427484251292036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	158464.0
1	84127.0
2	9790.0
3	12275.5
4	14761.0
5	40300.5
6	65840.0
7	81240.0
8	96640.0
9	63407.5
10	30175.0
11	33249.0
12	36323.0
13	44507.5
14	52692.0
15	73152.5
16	93613.0
17	135270.0
18	176927.0
19	222440.0
20	267953.0
21	315285.0
22	362617.0
23	434128.5
24	505640.0
25	600371.5
26	812628.0
27	930153.0
28	1013916.0
29	1097679.0
30	1171295.5
31	1244912.0
32	1345843.5
33	1446775.0
34	1534925.0
35	1623075.0
36	1775712.5
37	1928350.0
38	2011524.0
39	2094698.0
40	2182148.5
41	2269599.0
42	2287918.0
43	2306237.0
44	2314310.0
45	2322383.0
46	2293646.5
47	2264910.0
48	2216394.5
49	2167879.0
50	2082366.0
51	1996853.0
52	1868223.5
53	1739594.0
54	1610668.5
55	1481743.0
56	1352138.5
57	1222534.0
58	1090322.5
59	958111.0
60	849298.0
61	740485.0
62	638293.0
63	536101.0
64	462399.5
65	388698.0
66	324279.5
67	259861.0
68	237520.5
69	215180.0
70	187941.0
71	160702.0
72	135135.5
73	109569.0
74	91735.5
75	63365.5
76	52829.0
77	49109.5
78	45390.0
79	45901.5
80	46413.0
81	40447.0
82	34481.0
83	31862.5
84	29244.0
85	25633.0
86	22022.0
87	25449.5
88	28877.0
89	19844.5
90	10812.0
91	7174.0
92	3536.0
93	2694.0
94	1852.0
95	1427.0
96	1002.0
97	774.0
98	546.0
99	355.0
100	164.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8440699077893715
2	0.44693931553936594
3	0.4315458462339699
4	0.43296056968211627
5	0.4365249755771382
6	0.44566112597635893
7	0.44118455752955477
8	0.41404626912589176
9	0.4040182910088457
10	0.42406843729037874
11	0.4364552561464287
12	0.42894879744004194
13	0.4008692967218011
14	0.39892877256705406
15	0.44787181292510514
16	0.41460111959528795
17	0.4233886728409613
18	0.4044249876879843
19	0.4189353442043937
20	0.4222702569733302
21	0.4308457469505956
22	0.45278412781384353
23	0.4108943698625676
24	0.4174015167287852
25	0.40035802089659833
26	0.39779002186546597
27	0.393298928537264
28	0.39240419584315905
29	0.4236501207061219
30	0.42545992092828866
31	0.4331813478793629
32	0.42616583016422205
33	0.4204052622018517
34	0.4162976257425519
35	0.4255819299320302
36	0.43570286729002233
37	0.42711575740763863
38	0.4349998630303684
39	0.41605651271134825
40	0.437605626753135
41	0.43275141138998785
42	0.43792226916760724
43	0.39757795859705797
44	0.4255645000743529
45	0.4186274167187602
46	0.4172998425590006
47	0.42555869012179376
48	0.43135702277579835
49	0.47362733261969686
50	0.4259421469906958
51	0.40140381235724043
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4423689E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.228319748898905
#Duplication Level	Relative count
1	100.0
2	12.282819487869553
3	3.929367686442131
4	2.108811229403306
5	1.2394295589767945
6	0.8852125961398926
7	0.6497275761533489
8	0.5626574847297529
9	0.44128705426049786
10++	9.825068270867138
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	127577	0.3706081588176096	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43863	0.12742097455040335	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	14737090	4.7675242	5.9237895	18
TGTGT	6247820	3.2903473	25.00153	4
AAAAA	8450720	3.2882915	3.4176984	10
TGTTT	5912480	2.440433	19.746906	6
GTTTG	4408670	2.3217788	24.291994	7
CTTTG	3988365	2.2041898	25.743752	1
GTGTT	4099205	2.158802	24.335585	5
TTTGA	4952845	2.121246	20.007803	8
GAGGG	2376130	2.1137545	7.797227	11
TTGTG	3875265	2.0408666	24.331833	3
TTTGT	4711695	1.9447974	19.4836	2
TGAGG	2579180	1.7982495	9.345353	10
TTGAG	3150710	1.721713	14.920225	9
ACTTT	3151145	1.4162682	9.035406	3
AACTT	3032955	1.4144323	9.675819	2
TTGAT	2442455	1.0460751	5.133277	9
TAACT	2175120	1.014377	9.377543	1
>>END_MODULE