FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_2_TGACCA_L008_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_2_TGACCA_L008_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34423689
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51597 0.14988806109653152 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13341040 4.390675 5.3245187 18
AAAAA 8391425 3.177907 3.3259494 45
TGTGT 5352805 2.8910732 25.527302 4
TGTTT 5129305 2.16256 20.078823 6
CTTTG 3619095 2.0166278 25.687517 1
GAGGG 2176210 1.9838428 7.6361184 11
GTTTG 3609310 1.949404 24.833317 7
TTTGT 4317070 1.8201147 19.764532 2
TTTGA 4193965 1.8185582 20.199917 8
GTGTT 3292495 1.7782909 24.730665 5
TGAGG 2376925 1.6914294 9.3884945 10
TTGTG 3107970 1.6786281 24.772293 3
TTGAG 2692215 1.4954785 14.877455 9
TTGAT 2291230 0.9935074 5.3845863 9
Produced by FastQC (version 0.10.1)