##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_2_TGACCA_L008_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34423689	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.92507827966956	34.0	31.0	34.0	31.0	34.0
2	33.112996285784476	34.0	33.0	34.0	31.0	34.0
3	33.15897052753411	34.0	33.0	34.0	31.0	34.0
4	36.50920234028375	37.0	37.0	37.0	35.0	37.0
5	36.43689100258836	37.0	37.0	37.0	35.0	37.0
6	36.38173834303465	37.0	37.0	37.0	35.0	37.0
7	36.39545032492014	37.0	37.0	37.0	35.0	37.0
8	36.37577576302179	37.0	37.0	37.0	35.0	37.0
9	38.165739761360264	39.0	39.0	39.0	37.0	39.0
10	38.17078268398253	39.0	39.0	39.0	37.0	39.0
11	38.160473475111864	39.0	39.0	39.0	37.0	39.0
12	38.118807923229845	39.0	38.0	39.0	37.0	39.0
13	38.1020930383144	39.0	38.0	39.0	37.0	39.0
14	39.6312218309897	41.0	40.0	41.0	37.0	41.0
15	39.59934055876464	41.0	40.0	41.0	37.0	41.0
16	39.580537199252525	41.0	40.0	41.0	37.0	41.0
17	39.57282652071368	41.0	40.0	41.0	37.0	41.0
18	39.55279999188931	41.0	40.0	41.0	37.0	41.0
19	39.56529923332737	41.0	40.0	41.0	37.0	41.0
20	39.51896184049304	41.0	40.0	41.0	37.0	41.0
21	39.48320750864325	41.0	39.0	41.0	37.0	41.0
22	39.44582563478307	41.0	39.0	41.0	37.0	41.0
23	39.385847751529475	41.0	39.0	41.0	36.0	41.0
24	39.36458704353272	41.0	39.0	41.0	36.0	41.0
25	39.3131821519768	41.0	39.0	41.0	36.0	41.0
26	39.233648346056114	41.0	39.0	41.0	36.0	41.0
27	39.17782765815715	40.0	39.0	41.0	36.0	41.0
28	39.12356656487339	40.0	39.0	41.0	36.0	41.0
29	39.060037202869225	40.0	39.0	41.0	36.0	41.0
30	38.987019723539795	40.0	39.0	41.0	35.0	41.0
31	38.9086011670626	40.0	38.0	41.0	35.0	41.0
32	38.84481936261974	40.0	38.0	41.0	35.0	41.0
33	38.78196148588258	40.0	38.0	41.0	35.0	41.0
34	38.710205143905405	40.0	38.0	41.0	35.0	41.0
35	38.63840769651387	40.0	38.0	41.0	35.0	41.0
36	38.570866852765256	40.0	38.0	41.0	35.0	41.0
37	38.76274625883356	40.0	38.0	41.0	35.0	41.0
38	38.81077196578205	40.0	38.0	41.0	35.0	41.0
39	38.79699871794682	40.0	38.0	41.0	35.0	41.0
40	38.76185977627209	40.0	38.0	41.0	35.0	41.0
41	38.71616171061736	40.0	38.0	41.0	35.0	41.0
42	38.647976310731835	40.0	38.0	41.0	35.0	41.0
43	38.60188531798553	40.0	38.0	41.0	35.0	41.0
44	38.54314265969577	40.0	38.0	41.0	35.0	41.0
45	38.48865492016268	40.0	38.0	41.0	34.0	41.0
46	38.414743056736306	40.0	38.0	41.0	34.0	41.0
47	38.3550917218663	40.0	38.0	41.0	34.0	41.0
48	38.293912485672294	40.0	38.0	41.0	34.0	41.0
49	38.24155560433979	40.0	38.0	41.0	34.0	41.0
50	38.34922730100193	40.0	38.0	41.0	34.0	41.0
51	38.07917338551368	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	17.0
11	47.0
12	85.0
13	173.0
14	354.0
15	571.0
16	860.0
17	1386.0
18	2230.0
19	3819.0
20	6699.0
21	11183.0
22	17747.0
23	26769.0
24	38989.0
25	54776.0
26	74669.0
27	99687.0
28	132189.0
29	173257.0
30	226467.0
31	293179.0
32	385002.0
33	515901.0
34	736632.0
35	1105245.0
36	1700493.0
37	2811110.0
38	6373955.0
39	1.9614782E7
40	15407.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.539342081970727	28.592927486001248	27.920035334619076	22.94769509740895
2	22.25853831885217	28.10240918436001	30.271670803581234	19.36738169320658
3	21.003138274924574	26.911427186086883	31.20522033533361	20.88021420365493
4	21.65923297761608	26.932990825010066	30.689252973439306	20.71852322393454
5	24.408986972880182	27.236926567546632	28.1747150047922	20.179371454780984
6	21.889466872652726	26.95059788624049	30.157099083715288	21.0028361573915
7	24.25328747679389	27.413271753854602	27.80064275110884	20.532798018242666
8	21.503369380312492	27.208336096692022	30.335156699794723	20.953137823200763
9	21.10801953852186	27.257450530650562	30.7632340043509	20.871295926476677
10	21.538307530026778	27.223668561495547	30.421370585819552	20.816653322658127
11	23.903498547177787	27.140289932319572	28.075988601918873	20.88022291858377
12	21.43092217687651	29.749170694634152	27.897219266650943	20.922687861838398
13	22.847879551781915	27.436559748143203	28.562958490590596	21.15260220948429
14	22.447015228228796	27.550704357508533	28.72357225006006	21.27870816420261
15	22.541474273718894	27.57798851831365	28.50343552662238	21.377101681345074
16	22.51117486827088	27.523278167419214	28.502000019056656	21.463546945253253
17	22.5084272635626	27.58714500354683	28.409799426203275	21.494628306687293
18	22.40207317699158	27.51699563634798	28.51821023598023	21.562720950680216
19	22.20312526781556	27.671713534450777	28.691505812705305	21.43365538502836
20	22.051190700393764	27.692732625891765	28.693058595268656	21.56301807844582
21	22.144671943788477	27.69010607782333	28.552183933569697	21.6130380448185
22	22.113722968904437	27.730198461013238	28.58758480497499	21.56849376510733
23	22.171553897212657	27.771801912719667	28.481633588345968	21.57501060172171
24	22.073008147667146	27.778533560515523	28.468092106801656	21.68036618501567
25	22.119122677409734	27.879949182668945	28.376095891407804	21.624832248513517
26	22.122192465971384	27.84684535872112	28.349659697862855	21.681302477444646
27	22.12690154040569	27.80806879171478	28.28165094305365	21.783378724825877
28	22.13306656355163	27.86892479768801	28.287293671517887	21.71071496724247
29	22.15704133278685	27.9043451734647	28.237496568133647	21.701116925614798
30	22.19662163459587	27.832470250355794	28.183060217630945	21.787847897417386
31	22.274616023491568	27.80971176672698	28.118619764911756	21.7970524448697
32	22.288870451326144	27.85691724422198	28.066017448973433	21.788194855478437
33	22.22812553379635	27.788281986239525	28.05673434575014	21.92685813421398
34	22.250160347428192	27.831407029037475	28.03257954137338	21.88585308216095
35	22.266814863122995	27.824725248929603	28.01619861098651	21.892261276960888
36	22.30948402228639	27.805257644900514	27.976343941644334	21.90891439116876
37	22.332862513829877	27.77556579825054	27.979289015876464	21.912282672043123
38	22.296898510790054	27.826581863437124	28.001310376700182	21.87520924907264
39	22.257236869645205	27.720942401030875	28.027797950417227	21.9940227789067
40	22.293471286400226	27.785015365000987	27.99440315633816	21.92711019226063
41	22.308051636120883	27.744331655491543	28.009199612238145	21.938417096149426
42	22.266706127867888	27.753370164949796	27.970766988753038	22.00915671842928
43	22.312486613506184	27.785450304294812	27.90590805070311	21.9961550314959
44	22.334695805981045	27.731258777386085	27.935092253915382	21.998953162717484
45	22.336272664683264	27.702869452592253	27.88510755083645	22.075750331888027
46	22.27640099816147	27.809875344853364	27.86522095293157	22.048502704053597
47	22.291936811304563	27.80660434156258	27.86735030054449	22.034108546588367
48	22.28626339263058	27.727217149794726	27.841565731087105	22.144953726487593
49	22.348761052309065	27.777301845830642	27.832801417651666	22.041135684208626
50	22.276682780860586	27.73944709993168	27.876466116109754	22.107404003097983
51	22.329304694669943	27.737804100071088	27.839046843048447	22.09384436221052
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.487037179379676
2	41.62592001205875
3	41.8833524785795
4	42.377756201550625
5	44.58835842766117
6	42.89230303004422
7	44.78608549503656
8	42.456507203513254
9	41.979315464998535
10	42.354960852684904
11	44.78372146576156
12	42.35361003871491
13	44.0004817612662
14	43.72572339243141
15	43.918575955063964
16	43.974721813524134
17	44.00305557024989
18	43.964794127671794
19	43.63678065284392
20	43.61420877883958
21	43.757709988606976
22	43.68221673401177
23	43.74656449893436
24	43.75337433268282
25	43.74395492592325
26	43.80349494341603
27	43.91028026523157
28	43.843781530794104
29	43.858158258401645
30	43.98446953201326
31	44.07166846836127
32	44.077065306804585
33	44.15498366801033
34	44.13601342958914
35	44.15907614008388
36	44.21839841345515
37	44.245145185873
38	44.1721077598627
39	44.25125964855191
40	44.22058147866086
41	44.24646873227031
42	44.27586284629717
43	44.308641645002076
44	44.33364896869853
45	44.412022996571295
46	44.32490370221506
47	44.32604535789294
48	44.43121711911818
49	44.38989673651769
50	44.38408678395857
51	44.42314905688046
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41562.0
1	24742.0
2	7922.0
3	11129.0
4	14336.0
5	26483.5
6	38631.0
7	62232.0
8	85833.0
9	55339.5
10	24846.0
11	29831.5
12	34817.0
13	43827.0
14	52837.0
15	75248.5
16	97660.0
17	141633.5
18	185607.0
19	232884.0
20	280161.0
21	327745.0
22	375329.0
23	448006.5
24	520684.0
25	618913.5
26	837182.5
27	957222.0
28	1034392.5
29	1111563.0
30	1181992.0
31	1252421.0
32	1349308.5
33	1446196.0
34	1534823.5
35	1623451.0
36	1771550.5
37	1919650.0
38	2000443.0
39	2081236.0
40	2165189.0
41	2249142.0
42	2267091.5
43	2285041.0
44	2296486.0
45	2307931.0
46	2279307.5
47	2250684.0
48	2211179.5
49	2171675.0
50	2086041.0
51	2000407.0
52	1879034.0
53	1757661.0
54	1630084.0
55	1502507.0
56	1372240.0
57	1241973.0
58	1109524.0
59	977075.0
60	866767.0
61	756459.0
62	651238.0
63	546017.0
64	471516.5
65	397016.0
66	331170.5
67	265325.0
68	243292.0
69	221259.0
70	193487.0
71	165715.0
72	139231.0
73	112747.0
74	94106.5
75	63649.0
76	51832.0
77	48708.0
78	45584.0
79	46353.5
80	47123.0
81	41063.0
82	35003.0
83	31821.5
84	28640.0
85	25161.5
86	21683.0
87	25239.0
88	28795.0
89	18874.5
90	8954.0
91	5454.0
92	1954.0
93	1329.5
94	705.0
95	429.5
96	154.0
97	98.0
98	42.0
99	27.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22591710028521347
2	0.016052898920856505
3	0.0
4	0.0
5	1.1619905118245751E-4
6	0.0
7	2.904976279561438E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.4569217726781114E-4
15	0.0
16	4.938459675254444E-5
17	0.0
18	0.0
19	0.004877455173383655
20	6.10045018707902E-4
21	0.0
22	2.904976279561438E-6
23	1.0167416978465032E-4
24	6.274748763852706E-4
25	0.0
26	0.0030298902595825798
27	0.0027277727265081902
28	0.0
29	0.0
30	0.0
31	0.0042586952258370685
32	5.809952559122876E-6
33	0.0013043343495230857
34	0.0
35	0.007881200646450181
36	0.002062533158488621
37	6.681445442991308E-5
38	0.0
39	0.0
40	2.904976279561438E-6
41	0.0014495831635011574
42	8.714928838684315E-6
43	0.0
44	2.904976279561438E-6
45	0.004903599959899707
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0013972935904690517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4423689E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.37798087842046
#Duplication Level	Relative count
1	100.0
2	12.890935174990824
3	4.1690855102925966
4	2.1219097187468723
5	1.3638941714209456
6	0.8968071264137724
7	0.7393320655256398
8	0.5665098588729857
9	0.45574350248557033
10++	10.249224301871685
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51597	0.14988806109653152	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13341040	4.390675	5.3245187	18
AAAAA	8391425	3.177907	3.3259494	45
TGTGT	5352805	2.8910732	25.527302	4
TGTTT	5129305	2.16256	20.078823	6
CTTTG	3619095	2.0166278	25.687517	1
GAGGG	2176210	1.9838428	7.6361184	11
GTTTG	3609310	1.949404	24.833317	7
TTTGT	4317070	1.8201147	19.764532	2
TTTGA	4193965	1.8185582	20.199917	8
GTGTT	3292495	1.7782909	24.730665	5
TGAGG	2376925	1.6914294	9.3884945	10
TTGTG	3107970	1.6786281	24.772293	3
TTGAG	2692215	1.4954785	14.877455	9
TTGAT	2291230	0.9935074	5.3845863	9
>>END_MODULE