FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_1_CGATGT_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_1_CGATGT_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25095039
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 110383 0.4398598464023108 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47301 0.18848745363575647 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31346 0.12490915037031822 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12818800 5.7252717 7.3416505 18
TGTGT 5230475 3.6471717 31.787731 4
AAAAA 6051825 3.6053236 3.809186 44
TGTTT 4860785 2.7126157 25.582178 6
GTTTG 3837630 2.675951 31.163576 7
GTGTT 3623405 2.5265737 31.242018 5
TTTGA 4200130 2.4829414 26.637743 8
CTTTG 3298045 2.4416635 33.377018 1
TTGTG 3389805 2.363686 31.1664 3
GAGGG 1899670 2.1906927 9.357859 11
TTTGT 3767675 2.1025934 25.344814 2
TTGAG 2635420 1.946645 19.326975 9
TGAGG 2081585 1.9211631 11.7481365 10
AGGGG 1408300 1.6240466 5.288771 12
ACTTT 2417660 1.5174456 12.3122835 3
AACTT 2260610 1.5030221 13.386969 2
TGAGT 1615350 1.1931733 5.4727216 10
TTGAT 1892850 1.1189739 6.599864 9
TAACT 1652790 1.098898 13.098339 1
TTGAC 1221535 0.9579812 5.3119082 9
Produced by FastQC (version 0.10.1)