##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename 140701_JD_B_1_CGATGT_L003_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25095039 Filtered Sequences 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41698564405499 34.0 31.0 34.0 31.0 34.0 2 32.57673713916125 34.0 31.0 34.0 31.0 34.0 3 32.64976224982157 34.0 31.0 34.0 31.0 34.0 4 35.997573624013896 37.0 37.0 37.0 35.0 37.0 5 36.021349837312464 37.0 37.0 37.0 35.0 37.0 6 36.02110464940899 37.0 37.0 37.0 35.0 37.0 7 36.0453853847368 37.0 37.0 37.0 35.0 37.0 8 36.05805593687262 37.0 37.0 37.0 35.0 37.0 9 37.841996101301135 39.0 39.0 39.0 37.0 39.0 10 37.822409321619304 39.0 39.0 39.0 37.0 39.0 11 37.800101725285224 39.0 39.0 39.0 37.0 39.0 12 37.77417815529197 39.0 39.0 39.0 37.0 39.0 13 37.776287616050325 39.0 39.0 39.0 37.0 39.0 14 39.32123596221548 41.0 40.0 41.0 37.0 41.0 15 39.27792301896801 41.0 40.0 41.0 37.0 41.0 16 39.266459956487815 41.0 40.0 41.0 37.0 41.0 17 39.239327860777585 41.0 40.0 41.0 37.0 41.0 18 39.21025880852387 41.0 40.0 41.0 37.0 41.0 19 39.21175213156672 41.0 40.0 41.0 37.0 41.0 20 39.17810843011641 41.0 40.0 41.0 37.0 41.0 21 39.1331322896131 41.0 40.0 41.0 36.0 41.0 22 39.10530487719107 41.0 40.0 41.0 36.0 41.0 23 39.06730146942589 41.0 40.0 41.0 36.0 41.0 24 39.06121564505239 41.0 40.0 41.0 36.0 41.0 25 39.00291607436832 41.0 39.0 41.0 36.0 41.0 26 38.9536297194039 41.0 39.0 41.0 36.0 41.0 27 38.9052178002194 41.0 39.0 41.0 36.0 41.0 28 38.87539166605798 41.0 39.0 41.0 35.0 41.0 29 38.81914628624406 41.0 39.0 41.0 35.0 41.0 30 38.76646591384058 41.0 39.0 41.0 35.0 41.0 31 38.71781490357517 41.0 39.0 41.0 35.0 41.0 32 38.67498384840127 41.0 39.0 41.0 35.0 41.0 33 38.61347890314097 41.0 39.0 41.0 35.0 41.0 34 38.57113372089201 41.0 39.0 41.0 35.0 41.0 35 38.52782141522076 40.0 39.0 41.0 35.0 41.0 36 38.47234052913805 40.0 38.0 41.0 35.0 41.0 37 38.40959653419945 40.0 38.0 41.0 35.0 41.0 38 38.3554432013435 40.0 38.0 41.0 34.0 41.0 39 38.30625320008468 40.0 38.0 41.0 34.0 41.0 40 38.25405638939234 40.0 38.0 41.0 34.0 41.0 41 38.19486285715675 40.0 38.0 41.0 34.0 41.0 42 38.109667611992954 40.0 38.0 41.0 34.0 41.0 43 38.06558559243522 40.0 38.0 41.0 33.0 41.0 44 38.00560437463357 40.0 38.0 41.0 33.0 41.0 45 37.95311818403629 40.0 38.0 41.0 33.0 41.0 46 37.88569872316198 40.0 38.0 41.0 33.0 41.0 47 37.80574156509579 40.0 38.0 41.0 33.0 41.0 48 37.98920236784649 40.0 38.0 41.0 33.0 41.0 49 38.044071658944226 40.0 38.0 41.0 34.0 41.0 50 38.013031340576916 40.0 38.0 41.0 33.0 41.0 51 36.48790615547559 38.0 36.0 40.0 31.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 117092.0 3 2969.0 4 2072.0 5 1482.0 6 1497.0 7 2239.0 8 5087.0 9 8062.0 10 8664.0 11 10599.0 12 14617.0 13 19281.0 14 24070.0 15 27330.0 16 28581.0 17 30088.0 18 31170.0 19 32651.0 20 34168.0 21 36833.0 22 40447.0 23 45695.0 24 52252.0 25 60100.0 26 76066.0 27 85234.0 28 96282.0 29 116248.0 30 141513.0 31 178064.0 32 230047.0 33 309642.0 34 453307.0 35 706248.0 36 1127434.0 37 1823724.0 38 4102073.0 39 1.5000362E7 40 11749.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.81079758865975 26.948867009622656 29.88577576464419 23.3545596370734 2 22.08536646024073 28.5814045757114 30.623170807029087 18.71005815701878 3 22.687078758819407 27.315317998840417 29.880530047365223 20.117073194974953 4 21.696751417568926 25.439016363537558 31.148501335535634 21.715730883357878 5 26.394439416455036 25.463064999017448 28.290939306576735 19.85155627795078 6 21.329982014342377 25.126956404970862 33.00718705212123 20.535874528565532 7 24.823289216025696 25.51561511764306 29.78544809255756 19.87564757377368 8 22.54029650127724 25.56304758003187 31.707294613021226 20.189361305669664 9 22.616570046386226 25.786958789006952 31.436215758053198 20.160255406553627 10 22.58089621144196 27.21262976849203 30.071611118999584 20.134862901066427 11 25.55193307222236 25.586473143452142 28.450202387110714 20.411391397214786 12 21.69240862268843 29.218437533557896 28.56613111953326 20.523022724220414 13 23.782302617819585 25.94713000410675 29.337782967932334 20.932784410141327 14 24.387558626095522 26.289625917981716 28.20547827964412 21.117337176278642 15 23.42528893414354 27.57835826980366 27.746388405844485 21.249964390208312 16 23.83943239044272 26.59608447546512 28.113906241107422 21.45057689298474 17 23.614310945741522 26.64261663837817 28.259530905489292 21.483541510391017 18 23.420312613080267 26.60764868862236 28.411574180634535 21.56046451766284 19 23.11794860456406 26.83506962990449 28.615121587623587 21.431860177907865 20 22.8636345520792 26.88229355444502 28.66253317266126 21.59153872081452 21 22.928134313957415 26.96895737529194 28.51518649872454 21.58772181202611 22 22.68291410333414 27.106400921637565 28.690717951110056 21.519967023918237 23 22.671379817419478 27.225675216361868 28.535089365699275 21.56785560051938 24 22.52139399336466 27.24243118720392 28.504879258742616 21.7312955606888 25 22.57140043218288 27.40793484502419 28.371865361410762 21.648799361382164 26 22.574937191362718 27.347976977170553 28.36011035875489 21.71697547271184 27 22.575813593837303 27.32793078681152 28.27847681085854 21.81777880849264 28 22.546865346016194 27.404091840690874 28.27426594237777 21.774776870915158 29 22.616790447535315 27.39186899850612 28.161007285672902 21.830333268285663 30 22.63376817830673 27.35535375504773 28.116425912676203 21.89445215396934 31 22.726829026203674 27.29976955109589 28.03203252985375 21.94136889284669 32 22.74205377508755 27.378929896133 27.942218363678368 21.936797965101082 33 22.720868592957487 27.28554646587539 27.916613608408706 22.076971332758415 34 22.75970315177167 27.324940933366943 27.87130078114456 22.044055133716828 35 22.791702351517344 27.29780432580695 27.831920578389163 22.078572744286543 36 22.765925783197396 27.300497952687586 27.834772083643873 22.098804180471145 37 22.80620943520677 27.25510267274484 27.844109098538965 22.094578793509427 38 22.765338099223452 27.33309096916001 27.858454569495294 22.043116362121236 39 22.73650299609517 27.21036398580044 27.8608380224987 22.192294995605685 40 22.78706988057885 27.28272835653907 27.836200182048927 22.09400158083315 41 22.80323993303689 27.23853091713697 27.825277105068835 22.132952044757303 42 22.708619191706163 27.23221987135066 27.835794869374087 22.223366067569096 43 22.78280102763695 27.286575120538103 27.746141535032493 22.184482316792455 44 22.812124802094182 27.23953920099364 27.762051415817197 22.18628458109498 45 22.805207215480593 27.22291786774823 27.71857615189187 22.25329876487931 46 22.70444706814648 27.34066046561068 27.651324711643205 22.303567754599637 47 22.761781808101052 27.274746412357565 27.69852103465929 22.264950744882096 48 22.70964137847846 27.20137598584902 27.66092624573987 22.428056389932653 49 22.767158335094575 27.28308715194495 27.653016195366696 22.296738317593782 50 22.7198261990733 27.254229119298028 27.690089355934994 22.33585532569368 51 22.754073137279747 27.232994170235408 27.658459326622058 22.354473365862788 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.16535722573315 2 40.79542461725951 3 42.80415195379436 4 43.412482300926804 5 46.24599569440582 6 41.865856542907906 7 44.698936789799376 8 42.7296578069469 9 42.77682545293985 10 42.71575911250839 11 45.96332446943715 12 42.215431346908844 13 44.71508702796091 14 45.504895802374165 15 44.67525332435186 16 45.29000928342746 17 45.097852456132536 18 44.98077713074311 19 44.54980878247192 20 44.45517327289372 21 44.515856125983525 22 44.20288112725238 23 44.23923541793886 24 44.25268955405346 25 44.220199793565044 26 44.29191266407456 27 44.39359240232994 28 44.32164221693136 29 44.44712371582098 30 44.52822033227608 31 44.668197919050364 32 44.678851740188634 33 44.797839925715905 34 44.8037582854885 35 44.87027509580388 36 44.864729963668545 37 44.900788228716195 38 44.80845446134469 39 44.928797991700854 40 44.881071461412006 41 44.936191977794195 42 44.93198525927525 43 44.967283344429404 44 44.99840938318916 45 45.058505980359904 46 45.00801482274612 47 45.02673255298315 48 45.137697768411115 49 45.063896652688356 50 45.055681524766975 51 45.10854650314254 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 141097.0 1 75374.5 2 9652.0 3 11012.5 4 12373.0 5 41178.5 6 69984.0 7 84596.5 8 99209.0 9 62714.5 10 26220.0 11 27266.5 12 28313.0 13 33053.0 14 37793.0 15 50639.0 16 63485.0 17 89368.0 18 115251.0 19 144697.0 20 174143.0 21 206773.5 22 239404.0 23 287421.0 24 335438.0 25 395858.0 26 532953.0 27 609628.0 28 670262.5 29 730897.0 30 785441.5 31 839986.0 32 910350.0 33 980714.0 34 1047835.0 35 1114956.0 36 1220086.0 37 1325216.0 38 1391741.5 39 1458267.0 40 1530107.0 41 1601947.0 42 1630418.5 43 1658890.0 44 1675949.0 45 1693008.0 46 1686117.0 47 1679226.0 48 1654005.0 49 1628784.0 50 1573294.5 51 1517805.0 52 1426920.5 53 1336036.0 54 1243975.5 55 1151915.0 56 1056497.0 57 961079.0 58 861260.5 59 761442.0 60 677523.5 61 593605.0 62 512444.5 63 431284.0 64 371689.5 65 312095.0 66 260938.5 67 209782.0 68 191501.0 69 173220.0 70 152512.5 71 131805.0 72 111924.0 73 92043.0 74 77285.0 75 53181.0 76 43835.0 77 40482.5 78 37130.0 79 38413.5 80 39697.0 81 34373.0 82 29049.0 83 26662.0 84 24275.0 85 21285.5 86 18296.0 87 21491.0 88 24686.0 89 16592.0 90 8498.0 91 5476.5 92 2455.0 93 1833.0 94 1211.0 95 932.0 96 653.0 97 501.5 98 350.0 99 228.5 100 107.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8306980515152816 2 0.5784808702628436 3 0.5764685203318473 4 0.5648726029076903 5 0.5766398689398331 6 0.5296943352030655 7 0.4969826904831668 8 0.4724160819196177 9 0.49173464125718236 10 0.5138226722819598 11 0.5289013497847124 12 0.5134799750659881 13 0.503673255897311 14 0.5020753305065595 15 0.5459883923671128 16 0.5056377876121253 17 0.5119378375941157 18 0.5044901504237551 19 0.513926278417021 20 0.5143407029572657 21 0.5493954402700869 22 0.5626410861525261 23 0.5112604128648693 24 0.5081362894076396 25 0.5075425465567118 26 0.5335795652678603 27 0.5294273501627155 28 0.4778713434157245 29 0.533404231808526 30 0.5075385617053634 31 0.5157114918211524 32 0.5316030789990006 33 0.5193934944671734 34 0.5012185874666304 35 0.5085786079073239 36 0.5252990441656615 37 0.5218760568572935 38 0.5174449021577532 39 0.4856218792885717 40 0.5018003757635124 41 0.49522138618712647 42 0.5587877348985192 43 0.511037261189353 44 0.5175564779955114 45 0.5029838766140192 46 0.5057812422606716 47 0.5156796130103644 48 0.5630355864360282 49 0.5244183920176415 50 0.5195648430751593 51 0.5072994706244529 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2.5095039E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 29.86087374245937 #Duplication Level Relative count 1 100.0 2 12.131948606162782 3 4.049470848946637 4 2.0737979676911626 5 1.2907729490869098 6 0.91671221690254 7 0.6796314711518832 8 0.5657010016661508 9 0.45703899319709973 10++ 9.54645136222645 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 110383 0.4398598464023108 No Hit CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47301 0.18848745363575647 No Hit TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31346 0.12490915037031822 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 12818800 5.7252717 7.3416505 18 TGTGT 5230475 3.6471717 31.787731 4 AAAAA 6051825 3.6053236 3.809186 44 TGTTT 4860785 2.7126157 25.582178 6 GTTTG 3837630 2.675951 31.163576 7 GTGTT 3623405 2.5265737 31.242018 5 TTTGA 4200130 2.4829414 26.637743 8 CTTTG 3298045 2.4416635 33.377018 1 TTGTG 3389805 2.363686 31.1664 3 GAGGG 1899670 2.1906927 9.357859 11 TTTGT 3767675 2.1025934 25.344814 2 TTGAG 2635420 1.946645 19.326975 9 TGAGG 2081585 1.9211631 11.7481365 10 AGGGG 1408300 1.6240466 5.288771 12 ACTTT 2417660 1.5174456 12.3122835 3 AACTT 2260610 1.5030221 13.386969 2 TGAGT 1615350 1.1931733 5.4727216 10 TTGAT 1892850 1.1189739 6.599864 9 TAACT 1652790 1.098898 13.098339 1 TTGAC 1221535 0.9579812 5.3119082 9 >>END_MODULE