FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_1_CGATGT_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_1_CGATGT_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25095039
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55762 0.222203280895479 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11254710 5.2496605 6.5601583 18
AAAAA 6256240 3.5843666 3.8359377 47
TGTGT 4246100 3.085777 33.366882 4
TGTTT 4030720 2.3467577 26.798033 6
CTTTG 2934355 2.2139003 34.101505 1
GTTTG 3000185 2.1803305 32.675755 7
TTTGA 3400325 2.0628443 27.536245 8
GTGTT 2780885 2.020958 32.66026 5
GAGGG 1712835 2.0208201 9.101371 11
TTTGT 3372255 1.9633874 26.566893 2
TTGTG 2573115 1.8699652 32.643417 3
TGAGG 1869995 1.7675183 11.896084 10
TTGAG 2160540 1.6360518 19.518438 9
AGGGG 1283455 1.5142332 5.0654964 12
TGAGT 1515450 1.1475625 5.5301833 10
TTGAT 1728580 1.0486618 7.1708736 9
TTGAC 1125635 0.88491875 5.467537 9
Produced by FastQC (version 0.10.1)