##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_1_CGATGT_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25095039	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06953175087713	34.0	33.0	34.0	31.0	34.0
2	33.258322013366865	34.0	34.0	34.0	31.0	34.0
3	33.27179415819995	34.0	34.0	34.0	31.0	34.0
4	36.571907897812	37.0	37.0	37.0	35.0	37.0
5	36.50185875383577	37.0	37.0	37.0	35.0	37.0
6	36.46833053337753	37.0	37.0	37.0	35.0	37.0
7	36.47456375740241	37.0	37.0	37.0	35.0	37.0
8	36.449123868665836	37.0	37.0	37.0	35.0	37.0
9	38.28794037737897	39.0	39.0	39.0	37.0	39.0
10	38.24881164759297	39.0	39.0	39.0	37.0	39.0
11	38.225742904802814	39.0	39.0	39.0	37.0	39.0
12	38.18691873720539	39.0	39.0	39.0	37.0	39.0
13	38.2074466192302	39.0	39.0	39.0	37.0	39.0
14	39.779021144378376	41.0	40.0	41.0	38.0	41.0
15	39.76764419453582	41.0	40.0	41.0	38.0	41.0
16	39.702709248628786	41.0	40.0	41.0	38.0	41.0
17	39.703047124174624	41.0	40.0	41.0	38.0	41.0
18	39.701658921510344	41.0	40.0	41.0	37.0	41.0
19	39.70733693619683	41.0	40.0	41.0	38.0	41.0
20	39.686927802742204	41.0	40.0	41.0	37.0	41.0
21	39.64096310031636	41.0	40.0	41.0	37.0	41.0
22	39.60401771043273	41.0	40.0	41.0	37.0	41.0
23	39.57396782686809	41.0	40.0	41.0	37.0	41.0
24	39.538710619258254	41.0	40.0	41.0	37.0	41.0
25	39.48529667557002	41.0	39.0	41.0	37.0	41.0
26	39.412121176619806	41.0	39.0	41.0	37.0	41.0
27	39.35762909155073	41.0	39.0	41.0	36.0	41.0
28	39.2817851767435	41.0	39.0	41.0	36.0	41.0
29	39.241474061865375	41.0	39.0	41.0	36.0	41.0
30	39.13667848852516	40.0	39.0	41.0	36.0	41.0
31	39.103175810964075	40.0	39.0	41.0	36.0	41.0
32	39.02879453584431	40.0	39.0	41.0	35.0	41.0
33	38.95330539235265	40.0	39.0	41.0	35.0	41.0
34	38.89929738702538	40.0	38.0	41.0	35.0	41.0
35	38.836214121842964	40.0	38.0	41.0	35.0	41.0
36	38.776374366264186	40.0	38.0	41.0	35.0	41.0
37	38.940719199519876	40.0	39.0	41.0	35.0	41.0
38	38.95681070668988	40.0	39.0	41.0	35.0	41.0
39	38.926834542875184	40.0	39.0	41.0	35.0	41.0
40	38.895337401149284	40.0	39.0	41.0	35.0	41.0
41	38.85287187638959	40.0	38.0	41.0	35.0	41.0
42	38.794473242300995	40.0	38.0	41.0	35.0	41.0
43	38.75410080056062	40.0	38.0	41.0	35.0	41.0
44	38.68913632690509	40.0	38.0	41.0	35.0	41.0
45	38.637448939609136	40.0	38.0	41.0	35.0	41.0
46	38.56413381146768	40.0	38.0	41.0	35.0	41.0
47	38.48734807704423	40.0	38.0	41.0	35.0	41.0
48	38.42936374795034	40.0	38.0	41.0	34.0	41.0
49	38.37484157725358	40.0	38.0	41.0	34.0	41.0
50	38.46545813297999	40.0	38.0	41.0	35.0	41.0
51	38.18981245655765	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	19.0
11	23.0
12	78.0
13	166.0
14	271.0
15	454.0
16	719.0
17	1147.0
18	1765.0
19	2897.0
20	4824.0
21	7688.0
22	11969.0
23	18086.0
24	25970.0
25	36148.0
26	48375.0
27	64446.0
28	84451.0
29	108634.0
30	140998.0
31	182631.0
32	236969.0
33	318814.0
34	466141.0
35	727069.0
36	1151578.0
37	1902911.0
38	4369514.0
39	1.5164102E7
40	16176.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.604825333456052	27.550460235828915	27.915231725432143	23.929482705282883
2	22.57961411431775	27.174543984026105	30.530219286759785	19.71562261489636
3	21.268005656247542	26.11598782636267	31.61617924001566	20.999827277374123
4	22.03992589929826	26.032328541111255	31.025797568993617	20.901947990596867
5	25.502632734240866	26.327282635797	27.89460599203515	20.275478637926987
6	22.043022128796057	25.9970905006364	30.79178318870116	21.168104181866383
7	25.385977682680625	26.544776439677975	27.495573925985926	20.573671951655463
8	21.934263580941238	26.457069861497327	30.633755938773398	20.97491061878804
9	21.316100124343315	26.647389547478635	31.06517958372325	20.9713307444548
10	21.88505066678717	26.499914983196483	30.707802446531364	20.907231903484988
11	25.121136492356115	26.372284179355134	27.52132005054864	20.985259277740116
12	21.78129870510762	29.944246847885932	27.232823843438492	21.04163060356796
13	23.591212589866867	26.735654804122838	28.21525401893179	21.457878587078504
14	23.048341847443996	26.952094494335064	28.430830882015695	21.568732776205245
15	23.148232604858674	26.934686174426748	28.24084871914325	21.676232501571327
16	23.140533872053357	26.94581187939178	28.122622164484383	21.79103208407048
17	23.08408845270175	27.058583172554545	28.043036713351988	21.814291661391717
18	22.93778764290045	26.963790039082813	28.265687100471897	21.832735217544844
19	22.68731748948497	27.163151454523206	28.474180713309106	21.675350342682727
20	22.434737062994692	27.186330605508296	28.54509462907692	21.833837702420087
21	22.56943294648795	27.190597312879248	28.35422969456234	21.88574004607046
22	22.497087970255794	27.245153912691666	28.406136368227997	21.851621748824538
23	22.557016149686	27.285169789933384	28.283375052734527	21.874439007646092
24	22.42132000671527	27.34054328427224	28.256939548888525	21.98119716012396
25	22.488229645708063	27.447827437128115	28.141865011646328	21.9220779055175
26	22.482742905728966	27.409394900124507	28.133547874152576	21.97431431999395
27	22.480848130073834	27.378430062913665	28.052998924427687	22.08772288258481
28	22.49603298598208	27.45258581886984	28.053897433116077	21.997483762032
29	22.53765464344673	27.464021017127166	27.974764751003413	22.023559588422696
30	22.554061035627992	27.420788551032942	27.951519455158724	22.073630958180342
31	22.62342994628139	27.35651413943949	27.883646368444957	22.13640954583416
32	22.655630936509112	27.447237771267925	27.803441767114208	22.093689525108758
33	22.59101546887397	27.365700853280607	27.791022163458646	22.25226151438678
34	22.60914476752582	27.425224979382136	27.761287383525413	22.20434286956663
35	22.63966248478577	27.417309186940088	27.725391361951708	22.217636966322438
36	22.67728189642747	27.39384321933988	27.695044545797288	22.233830338435364
37	22.714044332251117	27.366793202952422	27.693418925642614	22.225743539153843
38	22.70366518551555	27.39094937581009	27.740014663005514	22.165370775668848
39	22.628495551368214	27.294241066692937	27.768803764877354	22.308459617061498
40	22.657689985714086	27.385279332447354	27.718390174798213	22.238640507040344
41	22.67772501650129	27.331495942379608	27.729559238165493	22.261219802953608
42	22.617187162211945	27.33098637488646	27.694785788801713	22.357040674099878
43	22.670311235138264	27.397931772613422	27.62545495670584	22.306302035542473
44	22.724585751430453	27.290537318076847	27.64973711761548	22.33513981287722
45	22.721674192257684	27.250676916660698	27.63247349406391	22.395175397017713
46	22.638846171839987	27.395847289155927	27.59645248402551	22.368854054978577
47	22.664804353289682	27.40439083508146	27.56945988302907	22.361344928599785
48	22.624179123640626	27.30705612465346	27.565374730937215	22.503390020768695
49	22.744312132768552	27.349457396738853	27.53839912342834	22.367831347064254
50	22.628658160565525	27.30669762808628	27.583749941317677	22.480894270030518
51	22.70324214529458	27.324527473152816	27.5183309967163	22.4538993848363
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.53430803873894
2	42.29523672921411
3	42.26783293362167
4	42.941873889895135
5	45.77811137216785
6	43.211126310662436
7	45.95964963433609
8	42.90917419972928
9	42.28743086879811
10	42.79228257027216
11	46.10639577009623
12	42.822929308675576
13	45.049091176945375
14	44.61707462364924
15	44.82446510643
16	44.93156595612383
17	44.89838011409347
18	44.77052286044529
19	44.36266783216769
20	44.268574765414776
21	44.45517299255841
22	44.34870971908033
23	44.431455157332095
24	44.40251716683923
25	44.410307551225564
26	44.45705722572292
27	44.568571012658644
28	44.49351674801408
29	44.56121423186942
30	44.627691993808334
31	44.759839492115546
32	44.74932046161787
33	44.84327698326075
34	44.81348763709245
35	44.857299451108204
36	44.91111223486284
37	44.93978787140496
38	44.8690359611844
39	44.93695516842971
40	44.89633049275443
41	44.9389448194549
42	44.97422783631182
43	44.97661327068074
44	45.059725564307676
45	45.11684958927539
46	45.007700226818564
47	45.026149281889474
48	45.12756914440932
49	45.1121434798328
50	45.10955243059605
51	45.157141530130886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38681.0
1	22879.0
2	7077.0
3	9707.5
4	12338.0
5	25694.5
6	39051.0
7	63601.5
8	88152.0
9	54346.0
10	20540.0
11	23441.5
12	26343.0
13	31530.5
14	36718.0
15	51060.5
16	65403.0
17	93150.0
18	120897.0
19	151428.5
20	181960.0
21	214900.5
22	247841.0
23	295478.5
24	343116.0
25	406512.5
26	547839.0
27	625769.0
28	681123.5
29	736478.0
30	788927.0
31	841376.0
32	910367.0
33	979358.0
34	1043484.0
35	1107610.0
36	1210896.5
37	1314183.0
38	1379756.5
39	1445330.0
40	1513901.5
41	1582473.0
42	1609930.0
43	1637387.0
44	1660218.0
45	1683049.0
46	1675373.0
47	1667697.0
48	1649901.5
49	1632106.0
50	1578706.5
51	1525307.0
52	1443407.5
53	1361508.0
54	1268313.5
55	1175119.0
56	1079255.5
57	983392.0
58	883271.0
59	783150.0
60	698389.0
61	613628.0
62	529648.5
63	445669.0
64	384222.5
65	322776.0
66	269840.5
67	216905.0
68	198682.0
69	180459.0
70	159611.0
71	138763.0
72	117689.0
73	96615.0
74	81012.0
75	54957.0
76	44505.0
77	41536.5
78	38568.0
79	40337.0
80	42106.0
81	36429.0
82	30752.0
83	27987.5
84	25223.0
85	22125.0
86	19027.0
87	22526.5
88	26026.0
89	16749.0
90	7472.0
91	4363.0
92	1254.0
93	852.0
94	450.0
95	266.0
96	82.0
97	53.5
98	25.0
99	16.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24967086124074162
2	0.012353039180373459
3	0.011097811005593575
4	0.0
5	1.0360613506119676E-4
6	0.0
7	0.0
8	0.0
9	1.1954554045522703E-5
10	0.0
11	0.0
12	5.379549320485216E-4
13	0.0
14	1.2751524315224216E-4
15	0.0
16	0.0
17	0.0
18	4.8216701316941573E-4
19	0.001004182539823907
20	0.001279137282870929
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.005762095049941943
27	0.0019605468634657235
28	0.004893397455967293
29	0.0018250619176164659
30	9.563643236418162E-5
31	7.850157156559909E-4
32	0.0017134860798582543
33	5.140458239574762E-4
34	0.0011755311478097324
35	4.4231849968433994E-4
36	0.0021040015120119956
37	0.001040046201960475
38	0.0023630168496649876
39	0.0014225919314172016
40	0.0015501071745694438
41	1.3946979719776486E-4
42	0.0028531535655314183
43	0.003558472254217258
44	0.003693957200066515
45	0.0
46	0.0038772603620978634
47	3.426972159716508E-4
48	2.3909108091045404E-4
49	0.0
50	0.009185082358309943
51	0.0042558212402060815
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.5095039E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.830037705967108
#Duplication Level	Relative count
1	100.0
2	12.718813647315663
3	4.217861849549394
4	2.123906763754476
5	1.40040734282025
6	0.9910677440396161
7	0.752785506050239
8	0.5823937381025279
9	0.4632526191078393
10++	9.868079498409234
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55762	0.222203280895479	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11254710	5.2496605	6.5601583	18
AAAAA	6256240	3.5843666	3.8359377	47
TGTGT	4246100	3.085777	33.366882	4
TGTTT	4030720	2.3467577	26.798033	6
CTTTG	2934355	2.2139003	34.101505	1
GTTTG	3000185	2.1803305	32.675755	7
TTTGA	3400325	2.0628443	27.536245	8
GTGTT	2780885	2.020958	32.66026	5
GAGGG	1712835	2.0208201	9.101371	11
TTTGT	3372255	1.9633874	26.566893	2
TTGTG	2573115	1.8699652	32.643417	3
TGAGG	1869995	1.7675183	11.896084	10
TTGAG	2160540	1.6360518	19.518438	9
AGGGG	1283455	1.5142332	5.0654964	12
TGAGT	1515450	1.1475625	5.5301833	10
TTGAT	1728580	1.0486618	7.1708736	9
TTGAC	1125635	0.88491875	5.467537	9
>>END_MODULE