FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_15_GGCTAC_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_15_GGCTAC_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24559423
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 118926 0.48423776079755626 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 9435680 4.4493656 5.6326346 18
TGTGT 5340030 3.767062 38.429535 4
AAAAA 5713950 3.6958747 3.8930511 43
GTTTG 4373930 3.0855381 38.14416 7
TGTTT 5166495 2.9798062 31.670082 6
GGGGG 2264500 2.9230256 5.6512637 13
TTTGA 4708515 2.892858 33.444683 8
GTGTT 3951310 2.7874057 37.979202 5
CTTTG 3439815 2.595127 40.773064 1
TTGTG 3583580 2.5279949 37.96055 3
GAGGG 2024680 2.2761495 11.031519 11
TTTGT 3876515 2.2358027 31.376093 2
TTGAG 2911665 2.188022 24.09541 9
TGAGG 2079945 1.9117416 13.89545 10
AACTT 2403990 1.682641 15.588832 2
ACTTT 2501095 1.6433793 14.2037 3
AGGGG 1431120 1.6088681 5.944777 12
TGAGC 1482500 1.4572581 5.7169213 10
TGAGT 1651435 1.2409999 6.963381 10
TAACT 1770460 1.2392099 15.353084 1
TTGAT 1994100 1.2251524 7.245362 9
TTGAC 1374805 1.1048819 7.454972 9
Produced by FastQC (version 0.10.1)