##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_15_GGCTAC_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24559423	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45534375950119	34.0	31.0	34.0	31.0	34.0
2	32.60778732464521	34.0	31.0	34.0	31.0	34.0
3	32.685079857128564	34.0	33.0	34.0	31.0	34.0
4	36.015996426300404	37.0	37.0	37.0	35.0	37.0
5	36.04003290305314	37.0	37.0	37.0	35.0	37.0
6	36.03997129737128	37.0	37.0	37.0	35.0	37.0
7	36.05861843741199	37.0	37.0	37.0	35.0	37.0
8	36.045368126115996	37.0	37.0	37.0	35.0	37.0
9	37.83857381339944	39.0	39.0	39.0	37.0	39.0
10	37.80717315712181	39.0	39.0	39.0	37.0	39.0
11	37.80543154454402	39.0	39.0	39.0	37.0	39.0
12	37.77246106311211	39.0	39.0	39.0	37.0	39.0
13	37.76855181003234	39.0	39.0	39.0	37.0	39.0
14	39.31905875801724	41.0	40.0	41.0	37.0	41.0
15	39.26419586486213	41.0	40.0	41.0	37.0	41.0
16	39.239378384418885	41.0	40.0	41.0	37.0	41.0
17	39.204703628419935	41.0	40.0	41.0	37.0	41.0
18	39.17036255289874	41.0	40.0	41.0	37.0	41.0
19	39.17291432294643	41.0	40.0	41.0	37.0	41.0
20	39.15091971012511	41.0	40.0	41.0	37.0	41.0
21	39.10899645321472	41.0	40.0	41.0	37.0	41.0
22	39.0741870034976	41.0	40.0	41.0	36.0	41.0
23	39.03757376547487	41.0	40.0	41.0	36.0	41.0
24	39.00394052417274	41.0	40.0	41.0	36.0	41.0
25	38.95612547574917	41.0	39.0	41.0	36.0	41.0
26	38.90883169364362	41.0	39.0	41.0	36.0	41.0
27	38.84697918188062	41.0	39.0	41.0	36.0	41.0
28	38.802010454398705	41.0	39.0	41.0	35.0	41.0
29	38.74164441892629	41.0	39.0	41.0	35.0	41.0
30	38.67972215796764	41.0	39.0	41.0	35.0	41.0
31	38.640022894674686	41.0	39.0	41.0	35.0	41.0
32	38.58163324928277	41.0	39.0	41.0	35.0	41.0
33	38.53098409518823	41.0	39.0	41.0	35.0	41.0
34	38.465938511666174	41.0	39.0	41.0	35.0	41.0
35	38.40622432375549	40.0	39.0	41.0	35.0	41.0
36	38.3377138379839	40.0	38.0	41.0	34.0	41.0
37	38.29618415709522	40.0	38.0	41.0	34.0	41.0
38	38.24628212967381	40.0	38.0	41.0	34.0	41.0
39	38.18323866973585	40.0	38.0	41.0	34.0	41.0
40	38.10428017791786	40.0	38.0	41.0	34.0	41.0
41	38.02519037193993	40.0	38.0	41.0	33.0	41.0
42	37.9616732038045	40.0	38.0	41.0	33.0	41.0
43	37.90747160468713	40.0	38.0	41.0	33.0	41.0
44	37.83024096290862	40.0	38.0	41.0	33.0	41.0
45	37.763835127559794	40.0	38.0	41.0	33.0	41.0
46	37.68774950453844	40.0	38.0	41.0	33.0	41.0
47	37.59708678823603	40.0	37.0	41.0	33.0	41.0
48	37.7858065313668	40.0	38.0	41.0	33.0	41.0
49	37.825612718995885	40.0	38.0	41.0	33.0	41.0
50	37.7951359443583	40.0	37.0	41.0	33.0	41.0
51	36.31958368891647	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125283.0
3	2624.0
4	1719.0
5	1355.0
6	1862.0
7	3974.0
8	11245.0
9	17944.0
10	17301.0
11	17500.0
12	20369.0
13	24526.0
14	28082.0
15	29794.0
16	30818.0
17	31323.0
18	31714.0
19	32584.0
20	34030.0
21	36435.0
22	39749.0
23	43936.0
24	49666.0
25	57219.0
26	67300.0
27	79246.0
28	94437.0
29	114201.0
30	138362.0
31	172201.0
32	222059.0
33	301346.0
34	445288.0
35	703193.0
36	1113100.0
37	1797285.0
38	3948627.0
39	1.4657942E7
40	13784.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.87703941305106	26.050239832412913	30.572983348365284	24.49973740617074
2	22.12761967735299	28.045511632263064	31.343577817787654	18.483290872596296
3	22.481634579903936	26.753181542383352	30.793782738228526	19.971401139484186
4	21.411899865017386	24.330346679448617	32.41640328387956	21.841350171654437
5	27.157570941596465	24.465871517079695	28.89189987235224	19.484657668971607
6	21.424801013369706	24.231422763106	34.191058441926884	20.15271778159741
7	25.70461595368045	24.842520486019477	30.046105952363554	19.406757607936523
8	22.874990605309527	24.88511460212442	32.43673873328953	19.803156059276525
9	22.913747321706722	25.14629179968169	32.106147986481346	19.833812892130233
10	22.8582997967493	26.673607680281798	30.522422116716484	19.945670406252418
11	26.68297349253088	24.85253931725364	28.15065332382004	20.313833866395438
12	22.029380028099617	29.388518325070915	28.171693809393457	20.410407837436008
13	24.735959070841833	25.350438397463066	28.856435135750903	21.0571673959442
14	25.243932010772618	25.817522309438445	27.67953396402128	21.259011715767656
15	24.096297668634314	27.245092672129957	27.163230161179463	21.495379498056266
16	24.583808053263926	26.088802242012488	27.579735961163472	21.74765374356012
17	24.162354423011585	26.236223026838662	27.8566771062286	21.744745443921154
18	24.00699112176002	26.19455745968394	27.922910839947306	21.875540578608735
19	23.587188130154846	26.50754900326432	28.267728997676954	21.637533868903883
20	23.171387636688532	26.60722836204333	28.36226030789063	21.85912369337751
21	23.322672689428803	26.721574545502946	28.123369702653424	21.83238306241483
22	23.009874501087907	26.86103313373437	28.435022103381524	21.694070261796202
23	23.04362546511374	27.05490533701653	28.168242598561612	21.73322659930811
24	22.83603299392743	27.073233173401213	28.17763810517182	21.91309572749954
25	22.788286095673193	27.34291738427791	28.040117649423436	21.828678870625463
26	22.822738476404165	27.25613540890443	27.98123142180987	21.939894692881534
27	22.809691191248167	27.275444761749547	27.8660076147765	22.048856432225787
28	22.79355123333506	27.307987577892	27.902207832530806	21.996253356242136
29	22.916662058051184	27.279939430093286	27.773429975795143	22.029968536060387
30	22.963595054898686	27.22203468846501	27.696074053621363	22.118296203014943
31	23.056948862333837	27.156586383443347	27.60604890309167	22.180415851131148
32	23.119757837826498	27.245874792579155	27.457726543796557	22.17664082579779
33	23.085666521482505	27.15364564642511	27.46801606005577	22.292671772036613
34	23.117692364559613	27.17444820088947	27.43721231505072	22.270647119500207
35	23.15409652163203	27.128187540736988	27.338790067451217	22.378925870179764
36	23.15305650966456	27.18119642278157	27.393069665757718	22.272677401796148
37	23.07672144361134	27.058485033783185	27.460791063561214	22.404002459044257
38	23.02199128717751	27.149084098472247	27.5738335804536	22.255091033896644
39	23.024335403246468	27.005809877266408	27.502938765561208	22.466915953925923
40	23.04295702225498	27.07085232118107	27.505092537588986	22.38109811897496
41	23.037982989082316	27.020360823201088	27.500442954250502	22.441213233466094
42	23.00500103554294	27.01186621219362	27.51063054346694	22.4725022087965
43	23.077501712574108	27.079182080584367	27.38581771201082	22.457498494830705
44	23.157975523751958	26.982142021244986	27.378362584571292	22.481519870431764
45	23.165078638200075	26.960963751153844	27.35626753693771	22.51769007370837
46	23.00864041551573	27.121126346665957	27.24548343033079	22.624749807487518
47	23.11584765045958	27.06278057111648	27.29728398375965	22.524087794664293
48	23.0085265483298	26.930068588529576	27.253571176488826	22.807833686651797
49	23.117327300397218	27.046271348016898	27.20872102308683	22.627680328499057
50	22.96547086562692	27.079856637717814	27.291920138570486	22.662752358084777
51	23.065435690992814	26.997288875504665	27.257039291549575	22.680236141952946
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.3767768192218
2	40.61091054994928
3	42.45303571938812
4	43.25325003667182
5	46.64222861056807
6	41.57751879496712
7	45.11137356161697
8	42.67814666458605
9	42.747560213836955
10	42.80397020300172
11	46.99680735892632
12	42.43978786553562
13	45.793126466786035
14	46.50294372654027
15	45.59167716669058
16	46.331461796824044
17	45.90709986693274
18	45.88253170036876
19	45.22472199905873
20	45.03051133006604
21	45.15505575184363
22	44.70394476288411
23	44.77685206442185
24	44.74912872142697
25	44.61696496629865
26	44.7626331692857
27	44.85854762347395
28	44.789804589577194
29	44.946630594111575
30	45.08189125791363
31	45.237364713464984
32	45.29639866362429
33	45.378338293519114
34	45.38833948405982
35	45.5330223918118
36	45.42573391146071
37	45.4807239026556
38	45.277082321074154
39	45.49125135717239
40	45.42405514122994
41	45.47919622254841
42	45.47750324433944
43	45.53500020740481
44	45.63949539418372
45	45.682768711908444
46	45.633390223003246
47	45.63993544512387
48	45.816360234981595
49	45.745007628896275
50	45.62822322371169
51	45.74567183294576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	142218.0
1	75307.5
2	8397.0
3	9444.0
4	10491.0
5	23708.5
6	36926.0
7	45869.0
8	54812.0
9	40461.0
10	26110.0
11	24872.0
12	23634.0
13	27975.5
14	32317.0
15	45846.5
16	59376.0
17	85093.5
18	110811.0
19	139791.0
20	168771.0
21	203215.0
22	237659.0
23	281134.0
24	324609.0
25	382398.0
26	520743.0
27	601299.0
28	657043.5
29	712788.0
30	759879.5
31	806971.0
32	872299.0
33	937627.0
34	998775.5
35	1059924.0
36	1176493.5
37	1293063.0
38	1360228.5
39	1427394.0
40	1509304.5
41	1591215.0
42	1609421.5
43	1627628.0
44	1646507.5
45	1665387.0
46	1644046.5
47	1622706.0
48	1597286.5
49	1571867.0
50	1509945.5
51	1448024.0
52	1354682.0
53	1261340.0
54	1170668.5
55	1079997.0
56	994930.5
57	909864.0
58	812865.5
59	715867.0
60	648838.5
61	581810.0
62	512171.0
63	442532.0
64	392862.5
65	343193.0
66	291235.5
67	239278.0
68	233440.0
69	227602.0
70	200264.0
71	172926.0
72	149501.5
73	126077.0
74	108066.0
75	75832.5
76	61610.0
77	59000.0
78	56390.0
79	55802.5
80	55215.0
81	49808.0
82	44401.0
83	39267.0
84	34133.0
85	29645.5
86	25158.0
87	29077.0
88	32996.0
89	21767.5
90	10539.0
91	6768.5
92	2998.0
93	2356.0
94	1714.0
95	1309.5
96	905.0
97	672.0
98	439.0
99	306.0
100	173.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8241846724167745
2	0.549271861965161
3	0.5341208545493923
4	0.5428221990394482
5	0.521710139525672
6	0.5391209720195788
7	0.5321379089402875
8	0.5323048509730868
9	0.5223494053585868
10	0.5713448561067579
11	0.5425656783549027
12	0.534751976868512
13	0.5265148126647763
14	0.5100201254728175
15	0.571800892879283
16	0.5261890721129727
17	0.5432945228395635
18	0.5276223305409089
19	0.5410835588441959
20	0.5418979102237053
21	0.5739059911953144
22	0.5814346696988768
23	0.5541498267284211
24	0.5484086495028813
25	0.531665585140172
26	0.5522238857158819
27	0.5638691104428635
28	0.5427448356583948
29	0.6051485818701848
30	0.6067039930050474
31	0.588303723585037
32	0.6161260384659689
33	0.6068831503085393
34	0.5847368645427867
35	0.5911620969271143
36	0.6146764930104425
37	0.5840243070857162
38	0.5953275042333038
39	0.5741462248522695
40	0.577505424292745
41	0.5855552876791934
42	0.5793295713828456
43	0.5642722143757205
44	0.5770086699512443
45	0.5565562350548707
46	0.5709702544721836
47	0.5620246045682751
48	0.6046151817166062
49	0.5957957562765216
50	0.580958274141864
51	0.5536693594145107
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4559423E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.89631720775054
#Duplication Level	Relative count
1	100.0
2	12.032588678036673
3	3.622521151947047
4	1.728854725491766
5	1.0722769370379268
6	0.7459898043611711
7	0.5691675340966718
8	0.47975551818934
9	0.37299490218058556
10++	12.826620759601782
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	118926	0.48423776079755626	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	9435680	4.4493656	5.6326346	18
TGTGT	5340030	3.767062	38.429535	4
AAAAA	5713950	3.6958747	3.8930511	43
GTTTG	4373930	3.0855381	38.14416	7
TGTTT	5166495	2.9798062	31.670082	6
GGGGG	2264500	2.9230256	5.6512637	13
TTTGA	4708515	2.892858	33.444683	8
GTGTT	3951310	2.7874057	37.979202	5
CTTTG	3439815	2.595127	40.773064	1
TTGTG	3583580	2.5279949	37.96055	3
GAGGG	2024680	2.2761495	11.031519	11
TTTGT	3876515	2.2358027	31.376093	2
TTGAG	2911665	2.188022	24.09541	9
TGAGG	2079945	1.9117416	13.89545	10
AACTT	2403990	1.682641	15.588832	2
ACTTT	2501095	1.6433793	14.2037	3
AGGGG	1431120	1.6088681	5.944777	12
TGAGC	1482500	1.4572581	5.7169213	10
TGAGT	1651435	1.2409999	6.963381	10
TAACT	1770460	1.2392099	15.353084	1
TTGAT	1994100	1.2251524	7.245362	9
TTGAC	1374805	1.1048819	7.454972	9
>>END_MODULE