FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_15_GGCTAC_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_15_GGCTAC_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24559423
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 7924865 4.014664 4.940556 18
AAAAA 5593980 3.5424829 3.74451 47
TGTGT 4217180 3.1740294 40.151733 4
TGTTT 4144000 2.5588384 33.053158 6
GTTTG 3366665 2.5338955 39.664207 7
TTTGA 3723030 2.4038396 34.299995 8
CTTTG 2979770 2.3415728 41.31775 1
GTGTT 2931600 2.2064471 39.524097 5
GAGGG 1779205 2.080329 11.072591 11
TTTGT 3360990 2.075345 32.695587 2
TTGTG 2617465 1.9700156 39.479263 3
TTGAG 2322275 1.8276302 24.680082 9
TGAGG 1810930 1.7371688 14.398616 10
AGGGG 1232385 1.4409617 5.998215 12
TGAGC 1408740 1.4109362 5.687761 10
TGAGT 1505155 1.1845568 7.111965 10
TTGAT 1792075 1.1570847 7.470654 9
GAGTG 1184690 1.1364363 5.0409403 11
TTGAC 1230475 1.0110757 7.3810005 9
Produced by FastQC (version 0.10.1)