##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_15_GGCTAC_L004_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24559423	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09479253645332	34.0	33.0	34.0	31.0	34.0
2	33.28181777723361	34.0	34.0	34.0	31.0	34.0
3	33.29527468947459	34.0	34.0	34.0	31.0	34.0
4	36.593593098665224	37.0	37.0	37.0	35.0	37.0
5	36.53866705256064	37.0	37.0	37.0	35.0	37.0
6	36.50440126382448	37.0	37.0	37.0	35.0	37.0
7	36.51580364897009	37.0	37.0	37.0	35.0	37.0
8	36.496347043658155	37.0	37.0	37.0	35.0	37.0
9	38.33216509198934	39.0	39.0	39.0	37.0	39.0
10	38.3101197043595	39.0	39.0	39.0	37.0	39.0
11	38.298116042872834	39.0	39.0	39.0	37.0	39.0
12	38.26918653585632	39.0	39.0	39.0	37.0	39.0
13	38.26534019142062	39.0	39.0	39.0	37.0	39.0
14	39.811268896667485	41.0	40.0	41.0	38.0	41.0
15	39.795477198303885	41.0	40.0	41.0	38.0	41.0
16	39.783032972720896	41.0	40.0	41.0	38.0	41.0
17	39.74864214033041	41.0	40.0	41.0	38.0	41.0
18	39.70486574542081	41.0	40.0	41.0	38.0	41.0
19	39.725137638616346	41.0	40.0	41.0	38.0	41.0
20	39.69965112779726	41.0	40.0	41.0	37.0	41.0
21	39.65903128098734	41.0	40.0	41.0	37.0	41.0
22	39.596192182528064	41.0	40.0	41.0	37.0	41.0
23	39.57455266762578	41.0	40.0	41.0	37.0	41.0
24	39.55196174600682	41.0	40.0	41.0	37.0	41.0
25	39.50415532970787	41.0	40.0	41.0	37.0	41.0
26	39.40515007213321	41.0	39.0	41.0	36.0	41.0
27	39.31479807159965	41.0	39.0	41.0	36.0	41.0
28	39.270584084976264	41.0	39.0	41.0	36.0	41.0
29	39.19555777022937	41.0	39.0	41.0	36.0	41.0
30	39.13048217785898	41.0	39.0	41.0	36.0	41.0
31	39.086876959609356	40.0	39.0	41.0	36.0	41.0
32	39.02109149714144	40.0	39.0	41.0	35.0	41.0
33	38.953032365621944	40.0	39.0	41.0	35.0	41.0
34	32.446692538338546	40.0	35.0	41.0	2.0	41.0
35	38.80958766824449	40.0	38.0	41.0	35.0	41.0
36	38.7405212247861	40.0	38.0	41.0	35.0	41.0
37	38.889483600652994	40.0	39.0	41.0	35.0	41.0
38	38.906149912398185	40.0	39.0	41.0	35.0	41.0
39	38.88730521071281	40.0	39.0	41.0	35.0	41.0
40	38.849021941598544	40.0	38.0	41.0	35.0	41.0
41	38.80211436563473	40.0	38.0	41.0	35.0	41.0
42	38.73203026797494	40.0	38.0	41.0	35.0	41.0
43	38.66938433366289	40.0	38.0	41.0	35.0	41.0
44	38.60473371055989	40.0	38.0	41.0	35.0	41.0
45	38.53201514546983	40.0	38.0	41.0	35.0	41.0
46	38.44229927551637	40.0	38.0	41.0	35.0	41.0
47	38.37323336138638	40.0	38.0	41.0	34.0	41.0
48	38.3207782202375	40.0	38.0	41.0	34.0	41.0
49	38.26122991570283	40.0	38.0	41.0	34.0	41.0
50	38.334492508231975	40.0	38.0	41.0	34.0	41.0
51	38.06653987758589	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	1.0
9	7.0
10	12.0
11	24.0
12	61.0
13	158.0
14	311.0
15	460.0
16	713.0
17	1110.0
18	1883.0
19	3154.0
20	5198.0
21	8605.0
22	13312.0
23	19732.0
24	28209.0
25	38167.0
26	51122.0
27	67726.0
28	87761.0
29	114572.0
30	146005.0
31	186430.0
32	241791.0
33	327412.0
34	489004.0
35	776855.0
36	1239977.0
37	2129429.0
38	5715704.0
39	1.2847734E7
40	16782.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.796133033950753	26.890402219491076	28.04513678140323	25.268327965154935
2	22.77844363267701	26.60647856003331	31.036226234871258	19.578851572418426
3	20.862745682466464	25.387981189940774	32.72181381096867	21.027459316624096
4	21.77367114854449	25.160973855126805	32.038293407788935	21.027061588539762
5	26.248804786659687	25.541133437866193	28.17301937427439	20.037042401199734
6	22.340031956840594	25.25048073293725	31.44831378845405	20.961173521768103
7	26.215518174022247	26.160415087927756	27.307583732728574	20.31648300532142
8	22.201230053328207	25.78650972378301	31.198912938630517	20.813347284258267
9	21.49323296398291	26.055608065384924	31.63477822748523	20.816380743146937
10	22.07703395176631	25.87094876739382	31.165033193436127	20.886984087403743
11	26.16152260580389	25.73009146021061	27.115669614876538	20.99271631910896
12	22.03478685042884	30.160672462710636	26.68548507936715	21.11905560749338
13	24.443664657756823	26.32434402062296	27.597757488032194	21.63423383358803
14	23.668535835490122	26.560896582114175	27.93144439160994	21.839123190785763
15	23.80349896656774	26.590062803999913	27.66684298731285	21.93959524211949
16	23.74407981816185	26.483321696930744	27.635824343267345	22.136774141640053
17	23.633132586217517	26.694364928687452	27.57300120609511	22.099501278999917
18	23.477551923175348	26.57146187841898	27.82329167611484	22.127694522290824
19	23.05739656682282	26.87443526573044	28.10795274113479	21.960215426311947
20	22.78443186552062	26.952457311395307	28.20002733777581	22.063083485308265
21	23.01044026275826	26.94005069881892	27.91575213997617	22.13375689844666
22	22.829090895172904	27.096776662871925	28.04189251514582	22.032239926809353
23	22.952017235909818	27.141415333739722	27.85432296190346	22.052244468447
24	22.70342714758883	27.124074359474974	27.903667626314956	22.26883086662124
25	22.733135872125334	27.343598422487368	27.80408155354464	22.119184151842656
26	22.78495933890096	27.24871419621679	27.66563490737905	22.3006915575032
27	22.811942551272608	27.27490386061282	27.54278260244271	22.370370985671858
28	22.81558979965095	27.338295267955786	27.634920920592084	22.21119401180118
29	22.94302646166019	27.299701185927894	27.540846955703636	22.216425396708285
30	22.87984220475547	27.21018027215787	27.61567434282452	22.29430318026214
31	22.977092258633387	27.132628491237387	27.43353855882315	22.456740691306077
32	23.028351938519332	27.315419713646595	27.294339645382514	22.36188870245156
33	22.95426891747416	27.146403235939214	27.327726714100738	22.57160113248589
34	23.0438136258938	27.192925855305855	27.3275304288423	22.435730089958042
35	23.074079329831644	27.166034064231255	27.261206408100136	22.498680197836972
36	23.099335492884073	27.170756295691152	27.19638489365577	22.533523317769003
37	23.07944883663395	27.107539757318342	27.316890595853653	22.496120810194057
38	22.993130169281116	27.260924654670056	27.361493621185584	22.384451554863244
39	22.95174442819768	27.048595563503262	27.324953847653504	22.67470616064555
40	22.951023727226815	27.178891784224735	27.352230547110167	22.517853941438283
41	22.98436734446082	27.014376518536288	27.444651285170668	22.556604851832226
42	22.91276642474682	27.10889536516688	27.35526157955003	22.623076630536268
43	22.958568027780792	27.184845404501694	27.30105338421131	22.555533183506206
44	23.09733009606944	26.986318041755297	27.271385813909387	22.644966048265875
45	23.157007477958114	26.941162760718125	27.210640269472623	22.691189491851137
46	22.96889582797297	27.134798861050257	27.235067123952376	22.661238187024395
47	22.971854835514662	27.223253575623502	27.19971882075568	22.605172768106158
48	22.99397660292718	26.994331334763633	27.229121301308822	22.78257076100036
49	23.214389980350514	27.085038890573237	27.145781362444115	22.55478976663213
50	22.933324538172624	27.070006221761833	27.176651617169455	22.82001762289608
51	23.000849424078584	27.05092590726338	27.141210553706337	22.807014114951702
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.06446099910569
2	42.35729520509543
3	41.89020499909056
4	42.80073273708426
5	46.28584718785942
6	43.30120547860869
7	46.532001179343666
8	43.01457733758647
9	42.30961370712984
10	42.96401803917005
11	47.15423892491285
12	43.153842457922224
13	46.07789849134485
14	45.50765902627589
15	45.74309420868723
16	45.880853959801904
17	45.73263386521744
18	45.60524644546617
19	45.01761199313477
20	44.847515350828886
21	45.144197161204914
22	44.86133082198226
23	45.00426170435682
24	44.97225801421007
25	44.85232002396799
26	45.08565089640416
27	45.18231353694447
28	45.026783811452134
29	45.15945185836847
30	45.17414538501761
31	45.433832949939465
32	45.390240640970895
33	45.52587004996005
34	45.47954371585185
35	45.57275952766861
36	45.632858810653076
37	45.57556964682801
38	45.37758172414436
39	45.626450588843234
40	45.4688776686651
41	45.54097219629305
42	45.53584305528309
43	45.51410121128699
44	45.74229614433531
45	45.84819696980925
46	45.63013401499737
47	45.57702760362082
48	45.77654736392754
49	45.769179746982644
50	45.75334216106871
51	45.807863539030286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30456.0
1	17951.0
2	5446.0
3	7307.5
4	9169.0
5	13830.5
6	18492.0
7	30441.5
8	42391.0
9	31178.5
10	19966.0
11	20272.0
12	20578.0
13	25953.5
14	31329.0
15	46198.0
16	61067.0
17	88364.0
18	115661.0
19	145437.5
20	175214.0
21	209406.5
22	243599.0
23	287880.5
24	332162.0
25	396585.5
26	542335.5
27	623662.0
28	673300.0
29	722938.0
30	767917.0
31	812896.0
32	878344.0
33	943792.0
34	1009295.0
35	1074798.0
36	1183331.0
37	1291864.0
38	1361757.0
39	1431650.0
40	1506961.0
41	1582272.0
42	1599515.0
43	1616758.0
44	1633726.0
45	1650694.0
46	1629427.0
47	1608160.0
48	1586038.5
49	1563917.0
50	1501461.0
51	1439005.0
52	1355977.0
53	1272949.0
54	1193003.5
55	1113058.0
56	1018372.0
57	923686.0
58	826836.5
59	729987.0
60	661339.5
61	592692.0
62	522470.0
63	452248.0
64	398816.0
65	345384.0
66	297171.0
67	248958.0
68	243643.0
69	238328.0
70	206885.5
71	175443.0
72	150441.0
73	125439.0
74	107316.5
75	74724.5
76	60255.0
77	59576.5
78	58898.0
79	57826.5
80	56755.0
81	50997.5
82	45240.0
83	39432.0
84	33624.0
85	30869.5
86	28115.0
87	29358.0
88	30601.0
89	19165.5
90	7730.0
91	4463.5
92	1197.0
93	857.5
94	518.0
95	315.5
96	113.0
97	83.0
98	53.0
99	33.0
100	13.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.244765522382183
2	0.011624865942493845
3	0.0017752860073300583
4	0.0
5	0.0
6	1.9951608797975427E-4
7	0.0
8	0.0
9	0.0
10	8.143513795092011E-4
11	0.0
12	5.822612363490787E-4
13	0.0
14	0.002267968591933125
15	0.0
16	0.0
17	0.0
18	5.700459656564407E-5
19	0.0012459576106490775
20	0.0
21	0.0011034461192349673
22	0.0
23	0.0
24	3.175970380085884E-4
25	0.0
26	3.1352528111104237E-4
27	0.0022394662936503027
28	7.532750260460109E-4
29	1.6287027590184022E-5
30	4.0717568975460054E-6
31	0.0010505132795668693
32	0.0027647229334337373
33	0.0
34	17.46048756927229
35	0.003330697142192632
36	0.0037460163457423245
37	6.962704294803669E-4
38	6.392658329147227E-4
39	0.0
40	0.0
41	0.0
42	1.9951608797975427E-4
43	3.2574055180368043E-5
44	0.0
45	1.8322906038957022E-4
46	0.0012459576106490775
47	0.0
48	1.0586567933619613E-4
49	4.0717568975460054E-6
50	0.0018852234435638004
51	0.0017508554659447823
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4559423E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.43644626144541
#Duplication Level	Relative count
1	100.0
2	11.261128664036999
3	3.2059710474844803
4	1.546401868241851
5	0.9753321427870557
6	0.7058238645655487
7	0.5429414342811913
8	0.41865363607224393
9	0.34277266453414973
10++	11.830235950572705
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	7924865	4.014664	4.940556	18
AAAAA	5593980	3.5424829	3.74451	47
TGTGT	4217180	3.1740294	40.151733	4
TGTTT	4144000	2.5588384	33.053158	6
GTTTG	3366665	2.5338955	39.664207	7
TTTGA	3723030	2.4038396	34.299995	8
CTTTG	2979770	2.3415728	41.31775	1
GTGTT	2931600	2.2064471	39.524097	5
GAGGG	1779205	2.080329	11.072591	11
TTTGT	3360990	2.075345	32.695587	2
TTGTG	2617465	1.9700156	39.479263	3
TTGAG	2322275	1.8276302	24.680082	9
TGAGG	1810930	1.7371688	14.398616	10
AGGGG	1232385	1.4409617	5.998215	12
TGAGC	1408740	1.4109362	5.687761	10
TGAGT	1505155	1.1845568	7.111965	10
TTGAT	1792075	1.1570847	7.470654	9
GAGTG	1184690	1.1364363	5.0409403	11
TTGAC	1230475	1.0110757	7.3810005	9
>>END_MODULE