FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_13_GTCCGC_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_13_GTCCGC_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 32392437
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 140028 0.4322860919664674 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47341 0.14614831233599374 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 13553920 6.012073 6.773175 18
AAAAA 7597450 3.5007756 3.8217835 44
GGGGG 3481590 3.225737 4.4673533 17
TGTGT 4572100 2.7229006 14.588869 7
TGTTT 4783890 2.4587753 13.010562 9
GTTTG 3817515 2.2735097 14.41629 10
TTTGA 4317170 2.2358584 12.736721 11
GTGTT 3449280 2.0542085 14.148592 8
CTTTG 3196455 2.001208 14.618559 4
AACTT 3398590 1.8644847 13.4167595 2
ACTTT 3394360 1.8480366 12.848633 3
TTTGT 3487030 1.7922285 12.173364 5
TTGTG 2825170 1.6825218 13.914469 6
TTGAG 2650260 1.5904207 8.523813 12
TGAGG 2144670 1.491291 5.1597304 13
TAACT 2367745 1.2989576 12.77956 1
GTAAC 1268615 0.806433 5.7569413 1
Produced by FastQC (version 0.10.1)