##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_13_GTCCGC_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	32392437	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.229654903704834	34.0	31.0	34.0	31.0	34.0
2	32.36731456790361	34.0	31.0	34.0	31.0	34.0
3	32.44428599181963	34.0	31.0	34.0	31.0	34.0
4	35.82814488456055	37.0	37.0	37.0	35.0	37.0
5	35.86102107723479	37.0	37.0	37.0	35.0	37.0
6	35.90863182044624	37.0	37.0	37.0	35.0	37.0
7	35.898053332634404	37.0	37.0	37.0	35.0	37.0
8	35.91334239532518	37.0	37.0	37.0	35.0	37.0
9	37.66087272161709	39.0	39.0	39.0	35.0	39.0
10	37.629376974631455	39.0	39.0	39.0	35.0	39.0
11	37.60233025999248	39.0	39.0	39.0	35.0	39.0
12	37.57706281870672	39.0	39.0	39.0	35.0	39.0
13	37.56785675001853	39.0	39.0	39.0	35.0	39.0
14	39.102692427865186	41.0	40.0	41.0	36.0	41.0
15	39.054803502434844	41.0	40.0	41.0	36.0	41.0
16	39.04228017793166	41.0	40.0	41.0	36.0	41.0
17	38.997868422187565	41.0	40.0	41.0	36.0	41.0
18	38.97048619713299	41.0	40.0	41.0	36.0	41.0
19	38.96591787768237	41.0	40.0	41.0	36.0	41.0
20	38.92549461468428	41.0	40.0	41.0	36.0	41.0
21	38.866715678107205	41.0	39.0	41.0	36.0	41.0
22	38.83193762173559	41.0	39.0	41.0	36.0	41.0
23	38.7834884420706	41.0	39.0	41.0	35.0	41.0
24	38.76910956097561	41.0	39.0	41.0	35.0	41.0
25	38.700434857679895	41.0	39.0	41.0	35.0	41.0
26	38.64952334398304	41.0	39.0	41.0	35.0	41.0
27	38.592382382344375	41.0	39.0	41.0	35.0	41.0
28	38.55729255566662	41.0	39.0	41.0	35.0	41.0
29	38.49186777765439	41.0	39.0	41.0	35.0	41.0
30	38.427285109792756	41.0	39.0	41.0	35.0	41.0
31	38.37133618566581	41.0	39.0	41.0	34.0	41.0
32	38.317037646781564	40.0	39.0	41.0	34.0	41.0
33	38.24580923009899	40.0	38.0	41.0	34.0	41.0
34	38.190487273310126	40.0	38.0	41.0	34.0	41.0
35	38.13319569009272	40.0	38.0	41.0	34.0	41.0
36	38.066136302125095	40.0	38.0	41.0	33.0	41.0
37	37.991785952998846	40.0	38.0	41.0	33.0	41.0
38	37.92440892915837	40.0	38.0	41.0	33.0	41.0
39	37.857074909183275	40.0	38.0	41.0	33.0	41.0
40	37.79552739424947	40.0	38.0	41.0	33.0	41.0
41	37.72100715978856	40.0	38.0	41.0	33.0	41.0
42	37.62074495969538	40.0	38.0	41.0	33.0	41.0
43	37.563438805175416	40.0	38.0	41.0	32.0	41.0
44	37.49001259151943	40.0	37.0	41.0	32.0	41.0
45	37.42425359969057	40.0	37.0	41.0	32.0	41.0
46	37.33908711468668	40.0	37.0	41.0	32.0	41.0
47	37.24560791767535	40.0	37.0	41.0	31.0	41.0
48	37.44044413824128	40.0	37.0	41.0	32.0	41.0
49	37.494450633646366	40.0	37.0	41.0	33.0	41.0
50	37.45217690783809	40.0	37.0	41.0	33.0	41.0
51	35.90577868531472	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	148367.0
3	3729.0
4	2659.0
5	1842.0
6	2689.0
7	7632.0
8	23512.0
9	38741.0
10	40928.0
11	41530.0
12	44083.0
13	47114.0
14	49081.0
15	49191.0
16	48915.0
17	49499.0
18	50697.0
19	51265.0
20	53587.0
21	56704.0
22	61799.0
23	68151.0
24	77425.0
25	89534.0
26	110546.0
27	122683.0
28	138358.0
29	165708.0
30	201653.0
31	253198.0
32	328751.0
33	446411.0
34	663651.0
35	1048397.0
36	1592945.0
37	2567599.0
38	5491842.0
39	1.8137962E7
40	14059.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.70146609595331	34.71575040558885	18.11082794843709	18.471955550020745
2	25.98268216739019	32.16291463725984	22.747457386997382	19.106945808352584
3	23.59820792267275	28.598618058039776	26.205209631953032	21.597964387334446
4	22.190873509641797	26.578376669123887	28.829641532969845	22.40110828826447
5	22.715776969715034	27.442037435824833	28.890753808285456	20.951431786174677
6	22.059973610412314	25.87392661380826	29.416076608317017	22.650023167462415
7	23.07298675090412	25.89250727680396	30.078619730085443	20.95588624220647
8	23.771169563629137	26.484846146483605	27.63150932620461	22.112474963682647
9	23.624360912826173	26.72410800627137	28.082594695244147	21.568936385658315
10	24.20554291255864	27.416155314368996	27.13019001315116	21.2481117599212
11	23.277870332484746	25.88576561362219	29.204524489913396	21.631839563979664
12	22.73198910784479	26.17202973948951	29.52120208754414	21.574779065121557
13	23.047243128079362	26.08307100870902	29.156367482981683	21.713318380229936
14	23.99108683740859	26.36311005017594	27.877819656815817	21.767983455599655
15	23.234036329785994	27.365304793968566	27.397996889828008	22.002661986417433
16	23.934502002721423	26.649967134780162	27.350722622198397	22.064808240300014
17	23.634799626851386	26.682211009174882	27.47308848987441	22.20990087409933
18	23.596029734148132	26.279312694502593	27.504170790816314	22.62048678053296
19	23.632920333926773	26.449806141046068	27.48872432126716	22.428549203760006
20	23.450320921553406	26.66075585201678	27.414318009895418	22.474605216534393
21	23.43081839647304	26.596826179787737	27.42700461510627	22.545350808632953
22	23.308839411398367	26.718723390596537	27.611346549198657	22.361090648806442
23	23.18837180238031	26.81281652896296	27.549535421395948	22.449276247260787
24	23.14011883578792	26.830280653670364	27.55709954151709	22.47250096902463
25	23.145402121753026	26.93214724022313	27.515376150550818	22.407074487473025
26	23.134470742593173	26.974621958551786	27.46900092089347	22.421906377961566
27	23.07058959634006	26.736636938424706	27.468288578774214	22.72448488646102
28	23.173905780164056	26.859294551268555	27.406023738609175	22.56077592995822
29	23.1065909393557	27.079733114947974	27.22645677126462	22.58721917443171
30	23.169383407749315	26.919777086818637	27.185808112716114	22.725031392715934
31	23.29612687252751	26.9181453311453	27.095531079321024	22.69019671700617
32	23.32277534797052	26.949070146803738	27.055516858976336	22.67263764624941
33	23.285489783862513	26.815768326358864	26.963272594602465	22.935469295176155
34	23.41458425073761	26.75252629637174	27.027041784368976	22.805847668521672
35	23.327188946955058	26.84996943630064	26.95426949531827	22.86857212142603
36	23.39364976912765	26.779727918176853	26.94486371083859	22.88175860185691
37	23.279377534438638	26.825393924332136	26.942122818171494	22.95310572305774
38	23.39611303710198	26.844428273623205	26.984482879311944	22.774975809962868
39	23.252182929586173	26.68494780498542	27.101430322316965	22.961438943111443
40	23.470331613661134	26.61548550074999	26.937896504525895	22.976286381062984
41	23.370592096072894	26.76460021595653	27.007204641660103	22.857603046310473
42	23.325727991453444	26.60048667896981	26.986216018860237	23.08756931071651
43	23.53939126394283	26.675304943886154	26.877475629062342	22.90782816310868
44	23.52548331909312	26.773464948971053	26.758391092660343	22.94266063927548
45	23.417577352729587	26.77490863562576	26.78616436416489	23.021349647479767
46	23.40372056159413	26.815068385837105	26.7465024467957	23.034708605773062
47	23.345973454504406	26.669500382176153	26.83635221567531	23.148173947644132
48	23.376548550811737	26.57764434515088	26.79949340248563	23.246313701551756
49	23.37392486235678	26.812127963780263	26.77411151563861	23.03983565822434
50	23.198087518043476	26.735081921777258	27.021478217209204	23.04535234297006
51	23.41113778579266	26.723849657719274	26.72732766002177	23.137684896466293
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	47.17342164597406
2	45.08962797574277
3	45.1961723100072
4	44.59198179790627
5	43.66720875588971
6	44.70999677787473
7	44.028872993110596
8	45.88364452731179
9	45.19329729848449
10	45.45365467247984
11	44.90970989646441
12	44.30676817296635
13	44.7605615083093
14	45.75907029300825
15	45.23669831620343
16	45.99931024302144
17	45.844700500950715
18	46.216516514681096
19	46.061469537686776
20	45.9249261380878
21	45.976169205106
22	45.66993006020481
23	45.6376480496411
24	45.612619804812546
25	45.55247660922605
26	45.55637712055474
27	45.79507448280108
28	45.734681710122274
29	45.69381011378741
30	45.89441480046525
31	45.986323589533676
32	45.995412994219926
33	46.22095907903867
34	46.22043191925928
35	46.19576106838109
36	46.275408370984564
37	46.232483257496376
38	46.17108884706485
39	46.213621872697615
40	46.446617994724114
41	46.22819514238336
42	46.41329730216995
43	46.44721942705151
44	46.4681439583686
45	46.438927000209354
46	46.43842916736719
47	46.49414740214854
48	46.6228622523635
49	46.41376052058112
50	46.243439861013535
51	46.54882268225896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206099.0
1	111421.5
2	16744.0
3	16251.5
4	15759.0
5	27030.5
6	38302.0
7	39180.0
8	40058.0
9	34659.0
10	29260.0
11	30986.5
12	32713.0
13	37906.5
14	43100.0
15	59880.5
16	76661.0
17	113331.0
18	150001.0
19	189630.0
20	229259.0
21	264915.0
22	300571.0
23	352556.5
24	404542.0
25	483904.0
26	681492.5
27	799719.0
28	862060.0
29	924401.0
30	965665.5
31	1006930.0
32	1079972.5
33	1153015.0
34	1224212.0
35	1295409.0
36	1473030.0
37	1650651.0
38	1725203.5
39	1799756.0
40	1923066.0
41	2046376.0
42	2033526.5
43	2020677.0
44	2029213.0
45	2037749.0
46	2026916.5
47	2016084.0
48	1996696.5
49	1977309.0
50	1919889.0
51	1862469.0
52	1763380.5
53	1664292.0
54	1570246.0
55	1476200.0
56	1384449.0
57	1292698.0
58	1174936.5
59	1057175.0
60	962375.0
61	867575.0
62	770746.5
63	673918.0
64	604645.0
65	535372.0
66	452657.5
67	369943.0
68	377229.5
69	384516.0
70	341766.0
71	299016.0
72	260864.0
73	222712.0
74	191898.0
75	137434.5
76	113785.0
77	110147.0
78	106509.0
79	109565.0
80	112621.0
81	95535.5
82	78450.0
83	74765.0
84	71080.0
85	62921.5
86	54763.0
87	64546.5
88	74330.0
89	49101.5
90	23873.0
91	15488.5
92	7104.0
93	5677.0
94	4250.0
95	3334.0
96	2418.0
97	1862.0
98	1306.0
99	936.5
100	567.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8164189684153743
2	0.5684475052000564
3	0.5666723994863369
4	0.5553117229185319
5	0.5671601676650633
6	0.5197633015385659
7	0.48826829546662387
8	0.46392001935513527
9	0.48289049693914665
10	0.504290553995675
11	0.5194175418169371
12	0.5043461225223653
13	0.49427895777029685
14	0.49288665746266636
15	0.536051054139582
16	0.49639673606527346
17	0.502953822214735
18	0.4956095152704935
19	0.5042380726093563
20	0.5043677325049671
21	0.5388418290355863
22	0.5517676857718362
23	0.5012558950103074
24	0.498801618414817
25	0.49811936039267435
26	0.5229090975773141
27	0.5189019893748655
28	0.46861247272009826
29	0.5225787735575438
30	0.4977519906884437
31	0.5056890285840488
32	0.5211154690213645
33	0.5090540115891866
34	0.4917289798232841
35	0.4982366717267985
36	0.5150492381910012
37	0.5113107112008893
38	0.5071955530854316
39	0.47575611554017994
40	0.49195434107041713
41	0.4854404748861594
42	0.5475383034626261
43	0.5008730896042184
44	0.5076894955449014
45	0.4938220609952873
46	0.4960725863262465
47	0.5058063399181729
48	0.5528852305863865
49	0.5152807737188777
50	0.5106562374420918
51	0.4979279576896299
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.2392437E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	43.64306227023722
#Duplication Level	Relative count
1	100.0
2	14.521332020154441
3	4.998644000057094
4	2.5678356813542873
5	1.6407599309153713
6	1.1062104797385062
7	0.8785452261665168
8	0.6537347093164333
9	0.5902168172540287
10++	15.779557230334433
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	140028	0.4322860919664674	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47341	0.14614831233599374	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	13553920	6.012073	6.773175	18
AAAAA	7597450	3.5007756	3.8217835	44
GGGGG	3481590	3.225737	4.4673533	17
TGTGT	4572100	2.7229006	14.588869	7
TGTTT	4783890	2.4587753	13.010562	9
GTTTG	3817515	2.2735097	14.41629	10
TTTGA	4317170	2.2358584	12.736721	11
GTGTT	3449280	2.0542085	14.148592	8
CTTTG	3196455	2.001208	14.618559	4
AACTT	3398590	1.8644847	13.4167595	2
ACTTT	3394360	1.8480366	12.848633	3
TTTGT	3487030	1.7922285	12.173364	5
TTGTG	2825170	1.6825218	13.914469	6
TTGAG	2650260	1.5904207	8.523813	12
TGAGG	2144670	1.491291	5.1597304	13
TAACT	2367745	1.2989576	12.77956	1
GTAAC	1268615	0.806433	5.7569413	1
>>END_MODULE