FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_13_GTCCGC_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_13_GTCCGC_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 32392437
Filtered Sequences 0
Sequence length 51
%GC 45

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65502 0.2022138686261858 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12148690 5.639479 6.36363 13
AAAAA 6894060 3.099661 3.3718998 47
TGTGT 3412650 2.106231 6.373957 1
TGTTT 3692640 1.9765092 6.069508 3
TTTGA 3273965 1.7412529 5.78941 5
GTTTG 2736285 1.6887897 6.289053 4
GTGTT 2369055 1.4621416 6.4137635 2
Produced by FastQC (version 0.10.1)