##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_13_GTCCGC_L003_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	32392437	
Filtered Sequences	0	
Sequence length	51	
%GC	45	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00369487482526	34.0	33.0	34.0	31.0	34.0
2	33.18566512917815	34.0	33.0	34.0	31.0	34.0
3	33.20471269265724	34.0	34.0	34.0	31.0	34.0
4	36.53166549339897	37.0	37.0	37.0	35.0	37.0
5	36.47361033688203	37.0	37.0	37.0	35.0	37.0
6	36.48064960348615	37.0	37.0	37.0	35.0	37.0
7	36.461690733549936	37.0	37.0	37.0	35.0	37.0
8	36.44666914070096	37.0	37.0	37.0	35.0	37.0
9	38.26057508423957	39.0	39.0	39.0	37.0	39.0
10	38.21241563269846	39.0	39.0	39.0	37.0	39.0
11	38.18856139166065	39.0	39.0	39.0	37.0	39.0
12	38.15451245610202	39.0	39.0	39.0	37.0	39.0
13	38.16626513775422	39.0	39.0	39.0	37.0	39.0
14	39.73326708947524	41.0	40.0	41.0	38.0	41.0
15	39.7206835348634	41.0	40.0	41.0	38.0	41.0
16	39.653220503292175	41.0	40.0	41.0	37.0	41.0
17	39.63912638002507	41.0	40.0	41.0	37.0	41.0
18	39.63820591825184	41.0	40.0	41.0	37.0	41.0
19	39.64089861469824	41.0	40.0	41.0	37.0	41.0
20	39.61741902901594	41.0	40.0	41.0	37.0	41.0
21	39.55420306906825	41.0	40.0	41.0	37.0	41.0
22	39.505507165144756	41.0	39.0	41.0	37.0	41.0
23	39.46352282787491	41.0	39.0	41.0	37.0	41.0
24	39.4215420099451	41.0	39.0	41.0	36.0	41.0
25	39.35888763170242	41.0	39.0	41.0	36.0	41.0
26	39.28006197866496	41.0	39.0	41.0	36.0	41.0
27	39.216908687666816	41.0	39.0	41.0	36.0	41.0
28	39.13629764873819	40.0	39.0	41.0	36.0	41.0
29	39.08455745395136	40.0	39.0	41.0	35.0	41.0
30	38.96448720421992	40.0	39.0	41.0	35.0	41.0
31	38.918663668312455	40.0	39.0	41.0	35.0	41.0
32	38.83245379160574	40.0	38.0	41.0	35.0	41.0
33	38.74814358672674	40.0	38.0	41.0	35.0	41.0
34	38.68189846290355	40.0	38.0	41.0	35.0	41.0
35	38.60332987604483	40.0	38.0	41.0	35.0	41.0
36	38.52937909549689	40.0	38.0	41.0	35.0	41.0
37	38.70258928033108	40.0	38.0	41.0	35.0	41.0
38	38.71378442443216	40.0	38.0	41.0	35.0	41.0
39	38.67322085090418	40.0	38.0	41.0	35.0	41.0
40	38.63121885519141	40.0	38.0	41.0	35.0	41.0
41	38.57115255020794	40.0	38.0	41.0	35.0	41.0
42	38.500274493086145	40.0	38.0	41.0	35.0	41.0
43	38.44854707906046	40.0	38.0	41.0	34.0	41.0
44	38.36949198975057	40.0	38.0	41.0	34.0	41.0
45	38.3047171782722	40.0	38.0	41.0	34.0	41.0
46	38.217542076256876	40.0	38.0	41.0	34.0	41.0
47	38.127711261736806	40.0	37.0	41.0	34.0	41.0
48	38.0583812511544	40.0	37.0	41.0	34.0	41.0
49	37.99058693854988	40.0	37.0	41.0	33.0	41.0
50	38.071628787917376	40.0	37.0	41.0	34.0	41.0
51	37.778378854298616	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	5.0
9	13.0
10	19.0
11	59.0
12	109.0
13	210.0
14	393.0
15	643.0
16	1113.0
17	1703.0
18	2826.0
19	4746.0
20	8012.0
21	13213.0
22	20281.0
23	29600.0
24	42124.0
25	56736.0
26	74961.0
27	97479.0
28	125309.0
29	161206.0
30	205985.0
31	265670.0
32	345535.0
33	467981.0
34	695835.0
35	1114428.0
36	1683102.0
37	2736079.0
38	5904774.0
39	1.8314289E7
40	17993.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.548791515597607	35.40744670355333	15.479520401611287	18.564241379237778
2	26.399704217829058	30.361592160228724	22.83792186781192	20.4007817541303
3	22.793586942819417	26.707368366583168	28.122710737783898	22.376333952813525
4	22.064881996992074	27.15741331842368	29.2650163987353	21.512688285848945
5	22.71179532799568	28.347177067158945	27.794957908790497	21.146069696054873
6	22.97698070694712	26.73752209504953	26.935451012839817	23.35004618516353
7	23.546598238348043	26.922577637489887	27.819379566903223	21.711444557258844
8	22.922986621846327	27.25007692382021	26.79072895935554	23.036207494977916
9	22.808741522249207	27.097190220581126	27.650655850643652	22.44341240652602
10	23.458096098172547	27.27594716013494	27.22525014095111	22.0407066007414
11	23.039442818087444	26.608201784879597	28.094270894159646	22.25808450287331
12	22.913247386137417	26.897275438243145	27.907349069340327	22.28212810627911
13	22.865250922615054	26.92198490653852	27.94106846607435	22.271695704772075
14	22.768422241669168	26.8290775674682	28.009598051790345	22.392902139072284
15	22.966021358627632	26.70568750353671	27.836226091911513	22.492065045924146
16	23.300784686252534	26.839610740000825	27.40787919105932	22.451725382687325
17	23.192108083748067	27.064731190184922	27.230343922564394	22.51281680350262
18	23.050001660889365	26.60957467525291	27.477346148237032	22.863077515620695
19	23.160885655835813	26.82278010746205	27.35956863043646	22.656765606265676
20	23.079244159984984	26.926161489057186	27.3171958295927	22.677398521365124
21	23.16199611656264	26.790216494053844	27.219563628386467	22.828223760997048
22	23.174761441999564	26.849560593418765	27.32430412691703	22.651373837664636
23	23.11724801687505	26.87842535589403	27.267454436972432	22.736872190258488
24	23.02238636753388	26.88437427539027	27.28977446185972	22.803464895216127
25	23.123067276475677	26.9198393439802	27.303271439564735	22.653821939979384
26	23.108161710559838	26.920621320770348	27.21122404232436	22.759992926345447
27	23.051888723555535	26.645690574067256	27.226102954399227	23.076317747977985
28	23.19404147274809	26.875856122435653	27.20178659741359	22.728315807402666
29	23.133694378237923	27.052775032579124	27.080349820702132	22.73318076848082
30	23.148412070374906	26.85136719882958	27.112471663491178	22.887749067304338
31	23.24036133401292	26.855282091146044	26.9490763051217	22.955280269719335
32	23.26420989243424	26.98731899259031	26.914069212228515	22.83440190274694
33	23.1421354015782	26.763552049536717	26.883234828429575	23.211077720455506
34	23.31411029550597	26.779326251674952	26.974954584515775	22.931608868303307
35	23.181363065563403	26.874182503057902	27.001064418874805	22.94339001250389
36	23.317638795277908	26.753598510434884	26.809544878977775	23.119217815309437
37	23.24707954899848	26.819717631028766	26.801935509803226	23.131267310169527
38	23.4378591772827	26.840681020657925	26.854909959435947	22.866549842623428
39	23.184663776407124	26.657587474641897	26.981599416350004	23.17614933260097
40	23.345619708682193	26.746927896777407	26.882384274279268	23.025068120261132
41	23.257677799416264	26.79527445754461	27.0174717287738	22.929576014265326
42	23.210258201166926	26.660748033557457	26.905031346506835	23.22396241876878
43	23.43209264812006	26.73461994126523	26.864342745561125	22.968944665053584
44	23.40064612475189	26.746424375853394	26.700476807514917	23.152452691879798
45	23.387570376381376	26.750327553311287	26.702057026459602	23.160045043847735
46	23.282077838810142	26.814457657843672	26.840230094636215	23.063234408709974
47	23.20349127114297	26.83984615122445	26.776513253385186	23.180149324247395
48	23.338670498023376	26.596960918710604	26.881290353850467	23.183078229415553
49	23.452526896942025	26.827857996605815	26.769038711104077	22.950576395348087
50	23.147594844235066	26.702244334116187	26.972726158733572	23.177434662915175
51	23.336966432546642	26.794964959177957	26.644463639030473	23.223604969244928
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.113032894835385
2	46.800485971959354
3	45.169920895632934
4	43.57757028284102
5	43.857865024050554
6	46.32702689211065
7	45.25804279560689
8	45.95919411682424
9	45.25215392877522
10	45.49880269891395
11	45.297527320960754
12	45.19537549241653
13	45.136946627387125
14	45.161324380741455
15	45.45808640455178
16	45.752510068939856
17	45.704924887250684
18	45.91307917651006
19	45.81765126210149
20	45.75664268135011
21	45.99021987755969
22	45.8261352796642
23	45.85412020713353
24	45.82585126275001
25	45.77688921645506
26	45.86815463690528
27	46.12820647153352
28	45.922357280150756
29	45.86687514671874
30	46.036161137679244
31	46.195641603732255
32	46.09861179518118
33	46.35321312203371
34	46.24571916380928
35	46.12475307806729
36	46.43685661058734
37	46.378346859168005
38	46.30440901990613
39	46.3608131090081
40	46.370687828943325
41	46.187253813681586
42	46.43422061993571
43	46.401037313173646
44	46.553098816631696
45	46.54761542022911
46	46.34531224752011
47	46.38364059539036
48	46.521748727438926
49	46.40310329229011
50	46.32502950715024
51	46.56057140179157
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81060.0
1	46210.5
2	11361.0
3	11743.5
4	12126.0
5	17600.0
6	23074.0
7	23603.0
8	24132.0
9	21662.5
10	19193.0
11	22746.5
12	26300.0
13	31952.5
14	37605.0
15	55513.0
16	73421.0
17	112225.5
18	151030.0
19	191569.0
20	232108.0
21	265599.0
22	299090.0
23	350098.0
24	401106.0
25	485353.5
26	692393.0
27	815185.0
28	871144.0
29	927103.0
30	966318.0
31	1005533.0
32	1074844.0
33	1144155.0
34	1214422.5
35	1284690.0
36	1462174.0
37	1639658.0
38	1708718.0
39	1777778.0
40	1899816.5
41	2021855.0
42	2007936.0
43	1994017.0
44	2003501.0
45	2012985.0
46	2002297.0
47	1991609.0
48	1984500.5
49	1977392.0
50	1920101.0
51	1862810.0
52	1773915.5
53	1685021.0
54	1600464.0
55	1515907.0
56	1438984.0
57	1362061.0
58	1226698.5
59	1091336.0
60	996172.5
61	901009.0
62	802644.5
63	704280.0
64	629436.5
65	554593.0
66	469079.5
67	383566.0
68	395036.5
69	406507.0
70	360929.5
71	315352.0
72	274524.0
73	233696.0
74	199670.0
75	140554.0
76	115464.0
77	112309.5
78	109155.0
79	115618.0
80	122081.0
81	102312.5
82	82544.0
83	77312.0
84	72080.0
85	64194.0
86	56308.0
87	66586.5
88	76865.0
89	47657.0
90	18449.0
91	10681.5
92	2914.0
93	2118.0
94	1322.0
95	767.0
96	212.0
97	135.5
98	59.0
99	47.0
100	35.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.24512203265225152
2	0.011536643568991119
3	0.010662982843803941
4	0.0
5	1.3583417635419033E-4
6	0.0
7	0.0
8	0.0
9	3.0871403716861438E-6
10	0.0
11	0.0
12	4.4763535389449085E-4
13	0.0
14	9.261421115058431E-5
15	0.0
16	0.0
17	0.0
18	4.7233247686798E-4
19	0.0010835862704618366
20	0.0012255947275593992
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.005634031178327213
27	0.001975769837879132
28	0.004568967750095493
29	0.0016330972566219702
30	1.1731133412407346E-4
31	7.625236718064776E-4
32	0.0016392715373653424
33	4.352867924077463E-4
34	0.0012379432890461438
35	3.519340023722204E-4
36	0.002164085400551987
37	0.0010835862704618366
38	0.002170259681295359
39	0.0012996860964798666
40	0.00141699743060394
41	1.543570185843072E-4
42	0.0029296962127301506
43	0.003278543074730685
44	0.0036088670945011022
45	0.0
46	0.003837315482005877
47	3.364983005137897E-4
48	2.531455104782638E-4
49	0.0
50	0.008736607251871786
51	0.00419542376512147
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.2392437E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	45.488270250898886
#Duplication Level	Relative count
1	100.0
2	15.498482878196793
3	5.296199971102442
4	2.756104609160526
5	1.729518855656697
6	1.2548764629388816
7	0.9268891778644704
8	0.6920965178442422
9	0.5700043346337235
10++	15.763617974281173
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65502	0.2022138686261858	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	12148690	5.639479	6.36363	13
AAAAA	6894060	3.099661	3.3718998	47
TGTGT	3412650	2.106231	6.373957	1
TGTTT	3692640	1.9765092	6.069508	3
TTTGA	3273965	1.7412529	5.78941	5
GTTTG	2736285	1.6887897	6.289053	4
GTGTT	2369055	1.4621416	6.4137635	2
>>END_MODULE