FastQCFastQC Report
Wed 17 Sep 2014
lane6_Undetermined_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461922
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTACTCTCTGTGGCTTCCCACTATATTTTACTCTCTGTGGCTTCCCA57320.10494474289453419No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAG4850.018.4496061
CTAGTAC5650.018.3621163
TACACCG11900.017.4425035
GTGTTAG7050.017.3683871
GTCCTAC3703.083187E-916.546911
GTATTAG6950.016.263041
CCTAGCA4107.075869E-1016.0582542
GTAGCAC11950.015.7848543
CGTATAC6050.015.5891733
GTATATA15500.015.49579051
GTATTAC11050.015.3431841
CTAGCAC5307.2759576E-1215.125913
CTACACT10650.015.0602584
ACTGTGC10200.014.8974128
TAGGACT5701.8189894E-1214.8970654
TCTATAC8650.014.7196043
GTGCTAC7400.014.63765051
CTACACC16000.014.4470494
CCTACAC10150.014.4027063
TACACTA11050.014.0881755
Produced by FastQC (version 0.11.2)