##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane6_Undetermined_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5461922 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63828483819432 33.0 33.0 33.0 33.0 33.0 2 32.690024500532964 33.0 33.0 33.0 33.0 33.0 3 32.69736880167824 33.0 33.0 33.0 33.0 33.0 4 36.628970168376625 37.0 37.0 37.0 37.0 37.0 5 36.48477916747987 37.0 37.0 37.0 37.0 37.0 6 36.48870617339464 37.0 37.0 37.0 37.0 37.0 7 36.44542873369484 37.0 37.0 37.0 37.0 37.0 8 36.46034124983843 37.0 37.0 37.0 37.0 37.0 9 36.492310032988385 37.0 37.0 37.0 37.0 37.0 10-11 36.461255396909735 37.0 37.0 37.0 37.0 37.0 12-13 36.42674208822462 37.0 37.0 37.0 37.0 37.0 14-15 38.58030698717411 40.0 37.0 40.0 37.0 40.0 16-17 38.5299400101283 40.0 37.0 40.0 37.0 40.0 18-19 38.4860735836213 40.0 37.0 40.0 37.0 40.0 20-21 38.45508641463573 40.0 37.0 40.0 37.0 40.0 22-23 38.38574388283099 40.0 37.0 40.0 37.0 40.0 24-25 38.32108962742419 40.0 37.0 40.0 37.0 40.0 26-27 38.22044199825629 40.0 37.0 40.0 37.0 40.0 28-29 38.12772765704088 40.0 37.0 40.0 37.0 40.0 30-31 38.01342869781004 40.0 37.0 40.0 35.0 40.0 32-33 37.88597585245633 40.0 37.0 40.0 33.0 40.0 34-35 37.79032664692026 40.0 37.0 40.0 33.0 40.0 36-37 37.6469149504515 40.0 37.0 40.0 33.0 40.0 38-39 37.4721165003821 40.0 37.0 40.0 33.0 40.0 40-41 37.329205543396625 40.0 37.0 40.0 33.0 40.0 42-43 37.192241394146606 40.0 37.0 40.0 33.0 40.0 44-45 37.00689171321011 40.0 37.0 40.0 33.0 40.0 46-47 36.7943445549021 37.0 37.0 40.0 33.0 40.0 48-49 36.76247967290635 37.0 37.0 40.0 33.0 40.0 50-51 36.96587373455718 37.0 37.0 40.0 33.0 40.0 52-53 36.86673290098247 37.0 37.0 40.0 33.0 40.0 54-55 36.640526173753486 37.0 37.0 40.0 33.0 40.0 56-57 36.368257364349034 37.0 37.0 40.0 33.0 40.0 58-59 36.092728988074164 37.0 37.0 40.0 33.0 40.0 60-61 35.84112634709906 37.0 37.0 40.0 33.0 40.0 62-63 35.59382100293632 37.0 37.0 40.0 30.0 40.0 64-65 35.30802499559679 37.0 35.0 38.5 27.0 40.0 66-67 34.91277575915584 37.0 33.0 37.0 27.0 40.0 68-69 34.65331645160806 37.0 33.0 37.0 27.0 40.0 70-71 34.342157028240244 37.0 33.0 37.0 27.0 40.0 72-73 34.104718998184154 37.0 33.0 37.0 27.0 37.0 74-75 33.856120611023 37.0 33.0 37.0 27.0 37.0 76-77 32.80450663704095 35.0 33.0 37.0 27.0 37.0 78-79 33.7520300546218 37.0 33.0 37.0 27.0 37.0 80-81 33.83775821038821 37.0 33.0 37.0 27.0 37.0 82-83 33.63935231956809 37.0 33.0 37.0 27.0 37.0 84-85 33.43760804346895 37.0 33.0 37.0 27.0 37.0 86-87 33.28451449874238 37.0 33.0 37.0 27.0 37.0 88-89 33.139629694455536 37.0 33.0 37.0 27.0 37.0 90-91 32.93728306629058 37.0 33.0 37.0 22.0 37.0 92-93 32.82000740764881 37.0 33.0 37.0 22.0 37.0 94-95 32.768333839260244 37.0 33.0 37.0 22.0 37.0 96-97 32.63757409937381 37.0 33.0 37.0 22.0 37.0 98-99 32.55416454134644 37.0 33.0 37.0 22.0 37.0 100-101 31.695580328682833 35.0 33.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.05874861556912947 2306 2 0.07938196597780234 2306 3 0.0698093879739119 2306 4 0.08475117435160939 2306 5 0.17721215969487503 2306 6 0.08013617738123457 2306 7 0.07288586833473687 2306 8 0.0920463559625091 2306 9 0.06665727474278782 2306 10-11 0.11913254472352719 2306 12-13 0.15781564008534588 2306 14-15 0.2769681878700183 2306 16-17 0.3339982972756559 2306 18-19 0.2400397982816358 2306 20-21 0.2378004819652304 2306 22-23 0.2978701638070689 2306 24-25 0.39967416628750385 2306 26-27 0.3296470483196927 2306 28-29 0.37006437013705806 2306 30-31 0.29272840362999375 2306 32-33 0.39108462461901894 2306 34-35 0.4042717157916016 2306 36-37 0.43976872877626505 2306 38-39 0.3751947922049439 2306 40-41 0.39230709296110433 2306 42-43 0.3907881367303787 2306 44-45 0.4162591006412981 2306 46-47 0.4446756206591189 2306 48-49 0.46065467624891454 2306 50-51 0.45213707275912896 2306 52-53 0.5407949639981169 2306 54-55 0.5831484122306705 2306 56-57 0.5601914603158704 2306 58-59 0.5917814917632853 2306 60-61 0.6464457548401725 2306 62-63 0.6396110354774223 2306 64-65 0.6832937006753284 2306 66-67 0.7573764500356504 2306 68-69 0.7239575649909327 2306 70-71 0.8538493439157477 2306 72-73 0.8160531454334432 2306 74-75 0.9449174098882409 2306 76-77 0.8611445738558814 2306 78-79 0.9462475826947383 2306 80-81 1.0165782209395218 2306 82-83 1.1174795751525934 2306 84-85 1.1785640632407564 2306 86-87 1.2145769729152534 2306 88-89 1.2544569629375957 2306 90-91 1.2984604490259528 2306 92-93 1.3665569549270131 2306 94-95 1.3518705795566746 2306 96-97 1.3989840747433249 2306 98-99 1.3851953915195807 2306 100-101 1.3825468762847066 2307 1 0.08559205822534466 2307 2 0.07258246742306085 2307 3 0.03381129995944576 2307 4 0.11362655257752152 2307 5 0.06848590732180071 2307 6 0.14300250975106366 2307 7 0.08935724040982507 2307 8 0.11978976209258718 2307 9 0.09252341771782113 2307 10-11 0.09629508378620244 2307 12-13 0.1121369415883251 2307 14-15 0.23536412670451057 2307 16-17 0.27439085180557043 2307 18-19 0.29794083048326314 2307 20-21 0.29399222615599996 2307 22-23 0.24307419160300725 2307 24-25 0.3013820451109126 2307 26-27 0.27393668256227244 2307 28-29 0.18686425199078371 2307 30-31 0.27799715258719715 2307 32-33 0.3479673216008834 2307 34-35 0.3512757269027631 2307 36-37 0.3293599629539088 2307 38-39 0.3637785700899485 2307 40-41 0.35116975467945366 2307 42-43 0.35302304038704335 2307 44-45 0.3669369198053474 2307 46-47 0.36101063975643655 2307 48-49 0.3646123510219823 2307 50-51 0.42766788548826185 2307 52-53 0.38964129041553264 2307 54-55 0.47666868649237415 2307 56-57 0.5483525947004964 2307 58-59 0.5715539023531591 2307 60-61 0.5820525960506799 2307 62-63 0.5224228942971294 2307 64-65 0.6278027735878098 2307 66-67 0.6275951897485896 2307 68-69 0.6494880958825746 2307 70-71 0.760116846023891 2307 72-73 0.8537934400776237 2307 74-75 0.8863748028238163 2307 76-77 0.8533103935069448 2307 78-79 0.9234739297652084 2307 80-81 0.8867416641368706 2307 82-83 1.0153120757500815 2307 84-85 0.9949142451950408 2307 86-87 1.0962102437182466 2307 88-89 1.2491074055351916 2307 90-91 1.3998149600040861 2307 92-93 1.3312088969000584 2307 94-95 1.3156480361649727 2307 96-97 1.3192096912910145 2307 98-99 1.3490824829115837 2307 100-101 1.3158305874305967 2201 1 0.036893130789245276 2201 2 0.013423833967401322 2201 3 0.033485955815521606 2201 4 0.052298150899417806 2201 5 0.07567975379337355 2201 6 0.07849218669576885 2201 7 0.10519553334342646 2201 8 0.06658682267987359 2201 9 0.00808161788969386 2201 10-11 0.09898247072859334 2201 12-13 0.09943216001874333 2201 14-15 0.18497875826643906 2201 16-17 0.20039649447053165 2201 18-19 0.20582963760385553 2201 20-21 0.18734331824364148 2201 22-23 0.2420260723075529 2201 24-25 0.22132358003656094 2201 26-27 0.22833274974207285 2201 28-29 0.12137629915363135 2201 30-31 0.19127572807245485 2201 32-33 0.20062447740465927 2201 34-35 0.26965175568638955 2201 36-37 0.26944336111733236 2201 38-39 0.2084491042675225 2201 40-41 0.2958828144385919 2201 42-43 0.25518892859459186 2201 44-45 0.3573822671934366 2201 46-47 0.18147493068602927 2201 48-49 0.24292163530505206 2201 50-51 0.28871924482803024 2201 52-53 0.46258531277595694 2201 54-55 0.5166721810821429 2201 56-57 0.5200793693074957 2201 58-59 0.5254513386680983 2201 60-61 0.5541637652274645 2201 62-63 0.5939259522950593 2201 64-65 0.5373155189472314 2201 66-67 0.6159150824973523 2201 68-69 0.6767877363963564 2201 70-71 0.6308844737353851 2201 72-73 0.6410502911894511 2201 74-75 0.6448773427650423 2201 76-77 0.7580122295629721 2201 78-79 0.8823141598207229 2201 80-81 0.883910644505022 2201 82-83 0.9604160482979225 2201 84-85 0.9914672925216408 2201 86-87 1.137726480369679 2201 88-89 1.0967827355760633 2201 90-91 1.1315097402040735 2201 92-93 1.192353289000657 2201 94-95 1.1897493592758224 2201 96-97 1.1156377537022664 2201 98-99 1.0781182935425377 2201 100-101 1.1699594179405182 1101 1 0.011177240659030474 1101 2 0.04155427740013096 1101 3 0.05739932025392136 1101 4 0.08169571780189244 1101 5 0.06700694015446373 1101 6 0.09792260008803311 1101 7 0.09499416270008254 1101 8 0.09028143163261149 1101 9 0.05708865089075488 1101 10-11 0.1145361808582166 1101 12-13 0.08394607630625472 1101 14-15 0.14523759307375883 1101 16-17 0.17378846240316648 1101 18-19 0.25239282796702156 1101 20-21 0.17028126831110768 1101 22-23 0.08281383007747678 1101 24-25 0.1823819576083352 1101 26-27 0.18108868597824568 1101 28-29 0.2738966964565108 1101 30-31 0.22145649814346768 1101 32-33 0.33909048102190553 1101 34-35 0.2242633334948394 1101 36-37 0.234659556163912 1101 38-39 0.07594210250679367 1101 40-41 0.1787325209331101 1101 42-43 0.19661337944882007 1101 44-45 0.3133739311705739 1101 46-47 0.32171034182903924 1101 48-49 0.34471700083961565 1101 50-51 0.4211958598178427 1101 52-53 0.36205102262189826 1101 54-55 0.3373136560589387 1101 56-57 0.4914937096099621 1101 58-59 0.4773277656696351 1101 60-61 0.5654332576947425 1101 62-63 0.5425122919148819 1101 64-65 0.5131411599483542 1101 66-67 0.37286142272782996 1101 68-69 0.5352057962249361 1101 70-71 0.5892412576405164 1101 72-73 0.5814832943832755 1101 74-75 0.68497166271667 1101 76-77 0.6776765332964558 1101 78-79 0.761633952685365 1101 80-81 0.7730262933847669 1101 82-83 0.8828482308050667 1101 84-85 0.9622586552454067 1101 86-87 1.0172941229556969 1101 88-89 0.9850073399542083 1101 90-91 1.0829800916658527 1101 92-93 1.0783073236200167 1101 94-95 1.0856517690638157 1101 96-97 0.9573460160213614 1101 98-99 1.0119981188873766 1101 100-101 0.9945208351404915 2304 1 0.03908424088390916 2304 2 0.03155165090666401 2304 3 0.027331221187473886 2304 4 0.02157047278232227 2304 5 -8.869920596481506E-4 2304 6 -0.128973801895377 2304 7 -0.009835335413391988 2304 8 -0.029680851794928742 2304 9 -0.0063534492619012894 2304 10-11 -0.0410630484212291 2304 12-13 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0.11759160280088565 2304 76-77 0.021194023322465227 2304 78-79 0.1455835533993124 2304 80-81 0.14414684600944128 2304 82-83 0.16244930128934243 2304 84-85 0.28938690714248594 2304 86-87 0.30979110960998213 2304 88-89 0.28392197206504477 2304 90-91 0.26832439331766267 2304 92-93 0.3320020498942782 2304 94-95 0.21946719413949722 2304 96-97 0.30859608853502607 2304 98-99 0.23910792628251443 2304 100-101 0.26467054076091756 2202 1 0.022679587509806254 2202 2 0.026437732936479108 2202 3 -0.026652079361340952 2202 4 0.003937390867918111 2202 5 0.002835304978212605 2202 6 -0.06243023678712234 2202 7 -0.011920075451790524 2202 8 -0.03454203227636299 2202 9 0.01930672884855511 2202 10-11 -0.034473409731937465 2202 12-13 0.0019138872175759047 2202 14-15 -0.00683230685281444 2202 16-17 0.041631184884273864 2202 18-19 -0.04509293126857017 2202 20-21 -0.07435509887967129 2202 22-23 -0.07401164011356087 2202 24-25 -0.05828906339902318 2202 26-27 -0.040358701054991286 2202 28-29 -0.1863386748582201 2202 30-31 -0.07648947396166506 2202 32-33 0.05800675319164128 2202 34-35 0.00515926315973303 2202 36-37 -0.056175047871391826 2202 38-39 -0.2314367459799911 2202 40-41 -0.09020838688447697 2202 42-43 -0.0884904056549658 2202 44-45 0.010986519033764353 2202 46-47 -0.11514420263157632 2202 48-49 -0.04048235644152243 2202 50-51 0.04775999083349802 2202 52-53 0.07671497261230087 2202 54-55 0.07608898029259592 2202 56-57 0.16207541778235424 2202 58-59 0.13199313197492302 2202 60-61 0.14909575227172667 2202 62-63 0.24786435174045351 2202 64-65 0.17734571572248825 2202 66-67 0.04577293758262613 2202 68-69 0.10096747525326322 2202 70-71 0.12256257063868503 2202 72-73 0.022914065886595836 2202 74-75 0.15485296157137185 2202 76-77 0.24014315528165753 2202 78-79 0.28524686550470335 2202 80-81 0.25788076054303843 2202 82-83 0.2925991562187775 2202 84-85 0.4699368400504227 2202 86-87 0.4308532847012714 2202 88-89 0.4468938186026108 2202 90-91 0.48985723095670863 2202 92-93 0.4127386588757034 2202 94-95 0.393723162428671 2202 96-97 0.2811675825336266 2202 98-99 0.2772760230419564 2202 100-101 0.35703649579860297 1102 1 0.06608308666552176 1102 2 0.030011510846541967 1102 3 0.0015814864828485042 1102 4 0.07541442252194486 1102 5 0.1250109006051403 1102 6 0.13732575942905356 1102 7 0.12064008808182791 1102 8 0.08569310787027717 1102 9 0.08766462464007674 1102 10-11 0.09707670190666562 1102 12-13 0.10735998160171789 1102 14-15 0.1838539325059898 1102 16-17 0.24418182991279735 1102 18-19 0.1863630922228552 1102 20-21 0.1602147396724689 1102 22-23 0.14107853847219332 1102 24-25 0.17125687411151347 1102 26-27 0.2268019897782665 1102 28-29 0.18814835892084858 1102 30-31 0.1610753211252529 1102 32-33 0.27436868526153546 1102 34-35 0.2285911082729868 1102 36-37 0.2527299524052822 1102 38-39 0.23301489546280152 1102 40-41 0.2103526138977614 1102 42-43 0.1990268951366687 1102 44-45 0.26555291298068795 1102 46-47 0.2541790339483043 1102 48-49 0.30463728956308245 1102 50-51 0.25570003505673355 1102 52-53 0.4017341847996505 1102 54-55 0.5111728246684777 1102 56-57 0.6031358322940932 1102 58-59 0.5869142351261161 1102 60-61 0.49497606828478524 1102 62-63 0.5208594245142777 1102 64-65 0.5463199486347747 1102 66-67 0.36052173352611305 1102 68-69 0.41185815782536395 1102 70-71 0.5356809992438372 1102 72-73 0.5682225757968737 1102 74-75 0.6891366883329724 1102 76-77 0.6990061130384149 1102 78-79 0.8073082603537074 1102 80-81 0.887832585185123 1102 82-83 0.9472325022238053 1102 84-85 1.0123360992638482 1102 86-87 0.9580236724911302 1102 88-89 0.9119940248532572 1102 90-91 0.937013237847232 1102 92-93 1.000223936626469 1102 94-95 1.0067647832136544 1102 96-97 1.071990288179343 1102 98-99 0.9508124587465332 1102 100-101 0.9936022817006744 2305 1 0.08982088763853113 2305 2 0.0939635757566748 2305 3 0.06828594343539152 2305 4 0.02929962407289821 2305 5 0.07639187693829541 2305 6 0.03991054755326928 2305 7 0.11400167569446751 2305 8 0.07365952543626975 2305 9 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-2.3902628572111695 1213 56-57 -2.0090914932058084 1213 58-59 -2.1670787090532215 1213 60-61 -2.220821441131072 1213 62-63 -2.191238363926317 1213 64-65 -2.605366891468769 1213 66-67 -2.7726622408326875 1213 68-69 -2.7056811613357823 1213 70-71 -2.7049047228166927 1213 72-73 -2.745388928334968 1213 74-75 -2.9220206043786625 1213 76-77 -3.6109261200676634 1213 78-79 -3.443990960555368 1213 80-81 -3.3251608622205353 1213 82-83 -3.2896665588672 1213 84-85 -4.891014421347705 1213 86-87 -3.966477932391612 1213 88-89 -4.6083142507564006 1213 90-91 -4.796435928971 1213 92-93 -4.473119249077534 1213 94-95 -4.095040069631477 1213 96-97 -4.465699989557379 1213 98-99 -4.1149738525743516 1213 100-101 -3.979871226660265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 11.0 11 21.0 12 118.0 13 285.0 14 707.0 15 1411.0 16 2834.0 17 5142.0 18 9003.0 19 13333.0 20 18385.0 21 24096.0 22 29530.0 23 34615.0 24 40738.0 25 47727.0 26 53567.0 27 60584.0 28 68685.0 29 78877.0 30 91722.0 31 109966.0 32 135816.0 33 175608.0 34 243912.0 35 384639.0 36 798847.0 37 1984812.0 38 1045965.0 39 961.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.498233633832726 25.248417288637555 15.953679225558517 23.299669851971203 2 17.856502022109176 25.21625435950919 34.60305945630823 22.324184162073404 3 20.29999249286364 26.62090975506594 30.101694232505167 22.977403519565247 4 16.22546056131889 21.50343780083275 35.82098023369795 26.450121404150405 5 16.176704830277693 34.265886623792866 32.281383732686045 17.276024813243396 6 26.058391167065366 36.30538846948016 20.92219552018502 16.714024843269456 7 23.618023106152013 31.01170247396429 25.40363996410055 19.966634455783147 8 22.402608459073566 35.40938519444254 23.36565773000786 18.82234861647603 9 23.123856400732194 20.58015475138605 25.623013290925794 30.672975556955954 10-11 23.889244432547898 27.385920452794693 28.377965073152637 20.34687004150478 12-13 23.011910302755716 25.81132087219827 28.96864577752558 22.208123047520434 14-15 22.040880593824568 27.05028898003573 25.85085819770015 25.057972228439553 16-17 22.006957841618632 30.575576789173663 26.22114772154129 21.196317647666415 18-19 21.281101174700844 30.163257765715667 27.503322664554513 21.052318395028976 20-21 21.99676022408374 28.420744269629182 27.33556123235986 22.24693427392722 22-23 21.97673992774601 28.731169741947415 27.457706878777728 21.83438345152885 24-25 22.178554228315846 28.784747050053184 27.443167655040018 21.593531066590955 26-27 22.08554973871835 29.503341497736514 27.097265394855512 21.313843368689632 28-29 22.027038123591318 29.113489392526986 27.346048673163025 21.51342381071867 30-31 21.73126968858215 28.84091900250498 27.69555295736556 21.732258351547312 32-33 21.406567138820364 29.673382373457546 27.186876707503327 21.73317378021876 34-35 21.772405507517874 29.21479385622188 27.564228377001772 21.448572259258473 36-37 22.208855159285935 28.89656319870133 27.71453350044095 21.180048141571785 38-39 22.025842167139103 28.67855459835007 27.828461622006333 21.467141612504488 40-41 22.357154605233035 28.552668258162218 27.443123937013702 21.647053199591053 42-43 22.230685024578804 28.981013176912914 27.176021965031577 21.612279833476713 44-45 21.58920539730135 28.983935609137294 27.55589413402727 21.870964859534084 46-47 21.64371790150262 28.56776802417087 27.842504594333622 21.946009479992888 48-49 21.524110313263698 28.261406525422224 27.710678804233492 22.50380435708059 50-51 21.892597436031714 28.42638799218805 27.800410445861836 21.880604125918403 52-53 21.862933373177004 29.083124959716876 27.518381922105622 21.535559745000498 54-55 21.631907446844373 29.116474104081995 27.61161326065641 21.640005188417224 56-57 21.036260769334675 28.792106181007483 27.907621849018266 22.264011200639576 58-59 20.544394334711924 29.184201634595063 28.03902232381707 22.232381706875945 60-61 21.110074684195805 29.2626858516841 27.54299278530066 22.084246678819433 62-63 21.387024661819744 28.492630292521376 27.920985596453 22.199359449205883 64-65 21.492967656679085 28.564235065530163 27.56043760472373 22.38235967306702 66-67 21.648577891266605 28.46301186359808 27.353410512149225 22.534999732986087 68-69 21.509727366212136 28.51657125511537 27.164688903157774 22.80901247551472 70-71 21.51686348958051 28.938020192880593 26.81334770019796 22.731768617340943 72-73 21.058455265993953 29.15527709944124 26.920745909807785 22.865521724757023 74-75 21.57055461131797 28.558788092869047 26.50884405532059 23.36181324049239 76-77 21.523833629252575 28.862930813674286 26.450321889663464 23.162913667409672 78-79 21.743685136607848 28.857031022803714 26.444491036257407 22.95479280433103 80-81 21.683654948974652 29.260631136168303 26.360269529405205 22.695444385451836 82-83 21.556569497782267 28.90278507561561 26.60556161758872 22.935083809013403 84-85 21.40631209085395 28.833383654805694 26.712824217622416 23.04748003671794 86-87 20.999874407359727 29.51291295506554 26.49336113609625 22.993851501478485 88-89 21.457907859784005 28.988787371960463 26.70304810221311 22.85025666604242 90-91 21.79507736451668 28.496554077441406 26.89158419913562 22.816784358906293 92-93 21.782424893110846 28.09202805600284 27.181877853788873 22.943669197097442 94-95 21.92446331404071 28.008311360298617 27.07862380755848 22.988601518102193 96-97 21.878862541625466 28.5596808396997 26.600209217096136 22.9612474015787 98-99 21.44913609411757 28.574456854921383 26.89449805757717 23.081908993383877 100-101 21.54021292282975 28.130527601576954 27.073972592055934 23.25528688353736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 161.0 1 88.5 2 10.5 3 9.5 4 12.0 5 10.0 6 12.0 7 25.5 8 45.0 9 65.5 10 93.0 11 126.0 12 225.0 13 372.0 14 604.0 15 926.5 16 1388.0 17 2137.5 18 3127.5 19 4440.5 20 6318.5 21 8864.5 22 12127.5 23 16489.0 24 21865.0 25 28089.0 26 35654.0 27 43473.5 28 50967.5 29 59072.5 30 67418.0 31 75903.0 32 83922.0 33 89994.5 34 98972.5 35 108593.0 36 121443.0 37 144746.5 38 176044.0 39 201557.0 40 222765.5 41 252877.5 42 270649.0 43 277577.0 44 294815.5 45 308672.5 46 305747.0 47 303874.0 48 280330.0 49 225798.0 50 180156.5 51 145791.0 52 116042.5 53 99432.0 54 88784.5 55 72085.0 56 57060.0 57 46937.5 58 40988.5 59 38633.5 60 37612.5 61 35654.5 62 33263.0 63 31769.5 64 30153.5 65 28986.0 66 27860.5 67 25794.5 68 23436.5 69 20889.0 70 17882.5 71 14823.5 72 11701.5 73 8755.5 74 6401.0 75 4452.5 76 3011.0 77 1916.5 78 1175.0 79 693.5 80 366.0 81 222.5 82 136.0 83 89.0 84 69.0 85 44.5 86 31.5 87 25.5 88 21.5 89 20.0 90 19.0 91 17.0 92 12.5 93 15.0 94 13.0 95 10.5 96 15.5 97 20.5 98 21.0 99 28.5 100 90.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08555596363331443 2 0.005455954881816327 3 0.00827547518986906 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.75325755292734E-4 12-13 0.025586231366907106 14-15 7.231886504420971E-4 16-17 0.02944018607369347 18-19 0.0034969375249225454 20-21 0.0022794173918997745 22-23 0.011571018407073554 24-25 2.746286014337078E-5 26-27 0.0 28-29 7.598057972999248E-4 30-31 0.0 32-33 0.0 34-35 1.0985144057348312E-4 36-37 0.028716997423251376 38-39 0.035353855291232646 40-41 0.037862129850993846 42-43 0.02608056284948778 44-45 0.009630309623608686 46-47 0.07528485393969375 48-49 0.007707909413572731 50-51 0.009959863945329136 52-53 0.010582355441912206 54-55 0.066093950078379 56-57 0.10628126875484492 58-59 0.16275406349632968 60-61 0.2020625706482077 62-63 0.13543767194039022 64-65 0.2118210402858188 66-67 0.4051778842685779 68-69 0.3592416735354331 70-71 0.6083298150358061 72-73 0.5066439982116185 74-75 0.5357363213901627 76-77 0.32200203518102233 78-79 0.27627637304231 80-81 0.1350806547585264 82-83 0.4507387692464301 84-85 0.6434822760193206 86-87 0.871341626628868 88-89 0.9658321740954924 90-91 1.0605515787299782 92-93 1.0504452461972176 94-95 1.0089030930870122 96-97 1.192538084579018 98-99 1.1933985875301771 100-101 1.4048443020607033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5461922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.282524099137134 #Duplication Level Percentage of deduplicated Percentage of total 1 91.88060967863004 44.36227751051868 2 5.232042915502256 5.052324763109148 3 1.168972361767326 1.693228086847685 4 0.38122560435446196 0.736261377178097 5 0.25677414131196846 0.6198851832965181 6 0.17778318087209374 0.5150292428926873 7 0.05892286596556009 0.19914612871806675 8 0.06677429614241673 0.25792252501593244 9 0.03510112378728644 0.1525293769649808 >10 0.22933657886880585 2.052144031704377 >50 0.05037697493708908 1.9255190095040546 >100 0.4606333991766125 41.60887990486064 >500 0.001026814074364384 0.32088065474851507 >1k 3.8179892412483534E-4 0.38692903247966687 >5k 3.826568557052343E-5 0.11704317216091958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAAATATAGTGGGAAGCCACAGAGAGTAAAATATAGTGGGAAGCCA 6335 0.11598481267216924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.055772308722094E-4 1.830857342891385E-5 0.0 2 8.421943777300371E-4 1.830857342891385E-5 3.66171468578277E-5 3 8.421943777300371E-4 3.66171468578277E-5 3.66171468578277E-5 4 8.60502951158951E-4 3.66171468578277E-5 9.154286714456926E-5 5 9.337372448746064E-4 3.66171468578277E-5 1.2816001400239695E-4 6 9.703543917324341E-4 3.66171468578277E-5 1.464685874313108E-4 7 9.88662965161348E-4 3.66171468578277E-5 1.464685874313108E-4 8 9.88662965161348E-4 5.4925720286741554E-5 1.464685874313108E-4 9 0.0010069715385902618 5.4925720286741554E-5 1.6477716086022465E-4 10-11 0.0010252801120191756 5.4925720286741554E-5 2.2885716786142312E-4 12-13 0.0010618972588770034 5.4925720286741554E-5 2.563200280047939E-4 14-15 0.001089360119020374 5.4925720286741554E-5 3.020914615770785E-4 16-17 0.0011534401260215725 8.238858043011234E-5 3.4786289514936316E-4 18-19 0.0012999087134528834 9.154286714456926E-5 5.217943427240448E-4 20-21 0.001492148734456479 1.0069715385902618E-4 0.0010344343987336326 22-23 0.0015928458883155052 1.0985144057348311E-4 0.002389268832473258 24-25 0.0016935430421745313 1.0985144057348311E-4 0.0033962403710635193 26-27 0.001748468762461273 1.464685874313108E-4 0.00684740646241378 28-29 0.0019407087834648683 1.464685874313108E-4 0.0264375800313516 30-31 0.002050560224038351 1.7393144757468158E-4 0.04456306772597632 32-33 0.002242800245041947 1.9224002100359544E-4 0.06255124111988417 34-35 0.0023068802520431453 2.0139430771805235E-4 0.10042252525759247 36-37 0.002389268832473258 2.0139430771805235E-4 0.20506517669054958 38-39 0.002581508853476853 2.0139430771805235E-4 0.4303796355934779 40-41 0.002929371748626216 2.0139430771805235E-4 0.7433097726404734 42-43 0.003332160364062321 2.1054859443250928E-4 1.0966652398185106 44-45 0.0037624118396417964 2.1970288114696621E-4 1.5379750937490502 46-47 0.004119429021505617 2.1970288114696621E-4 2.052244612793811 48-49 0.004558834783799549 2.1970288114696621E-4 2.5316546080299207 50-51 0.005135554846810335 2.1970288114696621E-4 3.0081901572376903 52-53 0.005803817776965691 2.1970288114696621E-4 3.4648242871282307 54-55 0.006417154986834305 2.1970288114696621E-4 3.937844590237649 56-57 0.007204423644277601 2.1970288114696621E-4 4.442447182511944 58-59 0.00815646946258112 2.1970288114696621E-4 4.93573507640717 60-61 0.008888812399737675 2.2885716786142312E-4 5.4066773564324055 62-63 0.009840858218041195 2.3801145457588005E-4 5.862405211938215 64-65 0.010921064050347113 2.47165741290337E-4 6.335077652152484 66-67 0.012266744197372281 2.563200280047939E-4 6.792178650665462 68-69 0.01349341861710951 3.112457482915355E-4 7.241809751219442 70-71 0.015086264505425013 3.3870860843490625E-4 7.6882551599967925 72-73 0.016486870372736925 3.4786289514936316E-4 8.097946107615599 74-75 0.01828111056877048 4.4856004900838933E-4 8.491232573442096 76-77 0.020221819352235347 4.760229091517601E-4 8.897133646361116 78-79 0.022382231016847182 4.760229091517601E-4 9.29864981594391 80-81 0.02483557985632164 4.760229091517601E-4 9.69091100165839 82-83 0.027069225814649128 4.94331482580674E-4 10.065312173992965 84-85 0.029458494647122385 5.034857692951309E-4 10.422292738709928 86-87 0.03170129489216433 5.217943427240448E-4 10.779621166322038 88-89 0.0343651923260713 5.309486294385017E-4 11.15202670415286 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 915 0.0 19.654167 1 TACACTG 1385 0.0 19.458418 5 TACACCG 1375 0.0 18.568357 5 CAGGGGT 860 0.0 18.142368 4 CTATACT 915 0.0 18.085287 4 CCTATAC 965 0.0 16.659496 3 GTATAGA 1185 0.0 16.374105 1 GTGTAGA 1635 0.0 15.919779 1 TAATACT 1570 0.0 15.659654 4 ACTGTCC 880 0.0 15.589223 8 GTCCTAC 365 4.3957698E-8 15.558957 1 GTACACT 1095 0.0 15.544146 4 GTGCTAG 520 5.456968E-12 15.471689 1 TATACCA 1015 0.0 15.371999 5 TGTACTG 1545 0.0 15.3011465 5 CTAACAC 810 0.0 15.1774435 3 GTGTAGG 1050 0.0 14.873623 1 TACACAG 1180 0.0 14.825254 5 AGGGGTG 865 0.0 14.758088 5 GTGTATG 1445 0.0 14.737941 1 >>END_MODULE