FastQCFastQC Report
Wed 17 Sep 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461922
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAAATATAGTGGGAAGCCACAGAGAGTAAAATATAGTGGGAAGCCA63350.11598481267216924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9150.019.6541671
TACACTG13850.019.4584185
TACACCG13750.018.5683575
CAGGGGT8600.018.1423684
CTATACT9150.018.0852874
CCTATAC9650.016.6594963
GTATAGA11850.016.3741051
GTGTAGA16350.015.9197791
TAATACT15700.015.6596544
ACTGTCC8800.015.5892238
GTCCTAC3654.3957698E-815.5589571
GTACACT10950.015.5441464
GTGCTAG5205.456968E-1215.4716891
TATACCA10150.015.3719995
TGTACTG15450.015.30114655
CTAACAC8100.015.17744353
GTGTAGG10500.014.8736231
TACACAG11800.014.8252545
AGGGGTG8650.014.7580885
GTGTATG14450.014.7379411
Produced by FastQC (version 0.11.2)