##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G50_Mov_M1_L36_GCTACGCT-AAGGAGTA_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265516 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.498022718028295 33.0 33.0 33.0 33.0 33.0 2 32.50873770318926 33.0 33.0 33.0 33.0 33.0 3 32.492859187393606 33.0 33.0 33.0 33.0 33.0 4 36.39243586073909 37.0 37.0 37.0 37.0 37.0 5 36.35901414604017 37.0 37.0 37.0 37.0 37.0 6 36.383905301375435 37.0 37.0 37.0 37.0 37.0 7 36.36240753852875 37.0 37.0 37.0 37.0 37.0 8 36.36147350818783 37.0 37.0 37.0 37.0 37.0 9 36.35891622350442 37.0 37.0 37.0 37.0 37.0 10-11 36.3197660404646 37.0 37.0 37.0 37.0 37.0 12-13 36.32285248346615 37.0 37.0 37.0 37.0 37.0 14-15 38.62974924298348 40.0 40.0 40.0 37.0 40.0 16-17 38.61475767938656 40.0 40.0 40.0 37.0 40.0 18-19 38.545780668584946 40.0 38.5 40.0 37.0 40.0 20-21 38.51372798626072 40.0 38.5 40.0 37.0 40.0 22-23 38.3259408095934 40.0 37.0 40.0 37.0 40.0 24-25 38.23050588288465 40.0 37.0 40.0 37.0 40.0 26-27 38.16761701743021 40.0 37.0 40.0 37.0 40.0 28-29 38.09602057879751 40.0 37.0 40.0 37.0 40.0 30-31 37.99667816628753 40.0 37.0 40.0 37.0 40.0 32-33 37.8889539613432 40.0 37.0 40.0 35.0 40.0 34-35 37.86221734283433 40.0 37.0 40.0 35.0 40.0 36-37 37.91331407523464 40.0 37.0 40.0 35.0 40.0 38-39 37.88058911704003 40.0 37.0 40.0 35.0 40.0 40-41 37.84096626945269 40.0 37.0 40.0 35.0 40.0 42-43 37.72052155049037 40.0 37.0 40.0 33.0 40.0 44-45 37.54829840762892 40.0 37.0 40.0 33.0 40.0 46-47 37.461407222163636 40.0 37.0 40.0 33.0 40.0 48-49 37.26087881709577 40.0 37.0 40.0 33.0 40.0 50-51 36.167394808599106 38.5 35.0 38.5 33.0 40.0 52-53 36.35376587474954 37.0 37.0 38.5 33.0 40.0 54-55 37.152717726991966 40.0 37.0 40.0 33.0 40.0 56-57 37.05296479308215 40.0 37.0 40.0 33.0 40.0 58-59 36.90254824568011 40.0 37.0 40.0 33.0 40.0 60-61 36.75421631841395 37.0 37.0 40.0 33.0 40.0 62-63 36.435165865710545 37.0 37.0 40.0 33.0 40.0 64-65 36.17791394868859 37.0 37.0 40.0 33.0 40.0 66-67 35.93244663221802 37.0 37.0 40.0 33.0 40.0 68-69 35.67651855255427 37.0 37.0 40.0 30.0 40.0 70-71 35.28942511939017 37.0 37.0 37.0 27.0 40.0 72-73 34.98820786694587 37.0 33.0 37.0 27.0 40.0 74-75 34.719030114945994 37.0 33.0 37.0 27.0 40.0 76-77 34.41751721176878 37.0 33.0 37.0 27.0 40.0 78-79 34.164180313050814 37.0 33.0 37.0 27.0 37.0 80-81 33.828257054188825 37.0 33.0 37.0 27.0 37.0 82-83 33.45692726615346 37.0 33.0 37.0 27.0 37.0 84-85 33.188329516865274 37.0 33.0 37.0 27.0 37.0 86-87 32.98616279244942 37.0 33.0 37.0 27.0 37.0 88-89 32.72261182000331 37.0 33.0 37.0 24.5 37.0 90-91 32.453388872986935 37.0 33.0 37.0 22.0 37.0 92-93 32.269115608852196 37.0 33.0 37.0 22.0 37.0 94-95 32.025107714789314 33.0 33.0 37.0 22.0 37.0 96-97 31.631438406725017 33.0 33.0 37.0 22.0 37.0 98-99 31.396405489688004 33.0 33.0 37.0 15.0 37.0 100-101 30.458559936124377 33.0 30.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.07673737353552212 2306 2 -0.07914574392452067 2306 3 0.11970624198967528 2306 4 0.11655920636738415 2306 5 0.2148602971221507 2306 6 0.24590010294941322 2306 7 0.18657977822973493 2306 8 0.154608233545396 2306 9 0.20682781511458614 2306 10-11 0.32170683302366143 2306 12-13 0.24365589648667907 2306 14-15 0.1274483545534295 2306 16-17 0.1521534958421853 2306 18-19 -0.059997042221567654 2306 20-21 0.15012580178279933 2306 22-23 0.03916298935317997 2306 24-25 -0.0313701507817612 2306 26-27 -0.14780651408915446 2306 28-29 -0.027299248368493068 2306 30-31 -0.10354769876129666 2306 32-33 0.23868419906454363 2306 34-35 0.1693711567219438 2306 36-37 0.1470288152116339 2306 38-39 0.09728876976695489 2306 40-41 0.2883609132767617 2306 42-43 0.05826558975096674 2306 44-45 0.30194295343604693 2306 46-47 -0.12595335320288825 2306 48-49 0.08600779841058426 2306 50-51 -0.013942078812632985 2306 52-53 -0.20317750073838425 2306 54-55 -0.1677801983731939 2306 56-57 -0.40798598847137413 2306 58-59 0.08350370652782857 2306 60-61 -0.14215234974502522 2306 62-63 -0.4740821732310181 2306 64-65 -0.3776483365674608 2306 66-67 -0.2584036447122102 2306 68-69 -0.6309866579022625 2306 70-71 -0.16974265870609884 2306 72-73 -0.4654377655879287 2306 74-75 -0.18379059510709084 2306 76-77 0.07796537942198967 2306 78-79 -0.3370011807840285 2306 80-81 -0.12746540569845877 2306 82-83 -0.6718536755822981 2306 84-85 -0.8494815771614483 2306 86-87 -0.37145073621595515 2306 88-89 -0.8559621549087737 2306 90-91 -0.9617377543574115 2306 92-93 -1.0181509267402653 2306 94-95 -1.354379098260324 2306 96-97 -0.8677130169414617 2306 98-99 -0.6858690188763923 2306 100-101 -0.9112797968577162 2307 1 0.4007943125482072 2307 2 0.4081584851541322 2307 3 0.3774197229780185 2307 4 0.36215302704762564 2307 5 0.3971673000667195 2307 6 0.4199281830074497 2307 7 0.47604335523651997 2307 8 0.48060149091747206 2307 9 0.5758373316084189 2307 10-11 0.547350156541782 2307 12-13 0.5675794231371327 2307 14-15 0.7489756878014191 2307 16-17 0.5517182122677369 2307 18-19 0.7137941923621298 2307 20-21 0.6078776901453651 2307 22-23 0.721288624141728 2307 24-25 0.8285709579111113 2307 26-27 0.6640613645435423 2307 28-29 0.645235211581145 2307 30-31 0.7009801039101475 2307 32-33 0.5319245012025604 2307 34-35 0.5982214959870831 2307 36-37 0.5980807505165444 2307 38-39 0.835926588228098 2307 40-41 0.8828430538477505 2307 42-43 0.9556005447289948 2307 44-45 0.7035880692982843 2307 46-47 0.6114468943112996 2307 48-49 0.9259984099413359 2307 50-51 0.6137837778851818 2307 52-53 0.534521470797479 2307 54-55 0.6835405885512387 2307 56-57 0.6218224014061491 2307 58-59 0.7685632379578635 2307 60-61 0.8382705581199517 2307 62-63 0.42465038341985917 2307 64-65 0.5762414769434088 2307 66-67 0.7371048262475455 2307 68-69 1.1019220605716882 2307 70-71 0.5718823999718339 2307 72-73 0.6344534135184645 2307 74-75 0.887423077918136 2307 76-77 0.7295144768486992 2307 78-79 0.9718154453415195 2307 80-81 0.7418748409622253 2307 82-83 0.7018500755379193 2307 84-85 0.6696687663711813 2307 86-87 0.5189068791128548 2307 88-89 0.1409439177238312 2307 90-91 0.09343017121763353 2307 92-93 0.48803692799450715 2307 94-95 0.25183862887661945 2307 96-97 0.45479157060601594 2307 98-99 0.11762391843423714 2307 100-101 0.3835928186844697 2201 1 0.21941973641719414 2201 2 0.1323748444296271 2201 3 0.23735686630777053 2201 4 0.15381624762490986 2201 5 0.3245099516257284 2201 6 0.15059562159813566 2201 7 0.2922289478260538 2201 8 0.13307026934771216 2201 9 0.2238399782050564 2201 10-11 0.2526681616695612 2201 12-13 0.24831302664387067 2201 14-15 0.0893528278229212 2201 16-17 0.12290752663379578 2201 18-19 0.21819829458692652 2201 20-21 0.3986104665906609 2201 22-23 0.18400882925296003 2201 24-25 0.13261942371211433 2201 26-27 0.23123943087387033 2201 28-29 0.3439780781761428 2201 30-31 0.3122198690247018 2201 32-33 0.448618736584244 2201 34-35 0.5083819459085177 2201 36-37 0.3495281080552104 2201 38-39 0.3844750953821787 2201 40-41 0.2706314915286754 2201 42-43 0.35631181757656094 2201 44-45 0.35400987063813005 2201 46-47 0.4580063186765315 2201 48-49 0.201223784186638 2201 50-51 0.24859479434370257 2201 52-53 0.3334835086768919 2201 54-55 0.17622109471284375 2201 56-57 0.2834715619387751 2201 58-59 0.4810973542426993 2201 60-61 0.44132241487290713 2201 62-63 0.354412189722062 2201 64-65 0.1454251672915987 2201 66-67 0.27277185130750325 2201 68-69 0.4531750586694443 2201 70-71 0.4608287236836901 2201 72-73 0.6362109191011243 2201 74-75 0.303662429501955 2201 76-77 0.4677702042492413 2201 78-79 0.7682135927238676 2201 80-81 0.9125596478433806 2201 82-83 0.8256611747025815 2201 84-85 0.656104958580265 2201 86-87 0.7026592569145222 2201 88-89 0.6326526266809083 2201 90-91 0.3292874204112479 2201 92-93 0.38736287291221316 2201 94-95 0.6455198793935324 2201 96-97 0.7941348838141842 2201 98-99 0.7507394589188969 2201 100-101 0.6146324349279553 1101 1 -0.10099662161952949 1101 2 -0.13929097756562214 1101 3 0.018203616921780963 1101 4 -0.1613761425353175 1101 5 -0.07510409850876698 1101 6 -0.1073475385125775 1101 7 0.26714220656840126 1101 8 0.01619373431983462 1101 9 0.11101786600670493 1101 10-11 0.11130915032497768 1101 12-13 0.16210781047296052 1101 14-15 0.12784060607717862 1101 16-17 0.21421975617650446 1101 18-19 0.08693635548124234 1101 20-21 -0.008615372557905232 1101 22-23 0.2763444107117792 1101 24-25 0.08693894341895003 1101 26-27 -0.06799841560384579 1101 28-29 0.035802958297772136 1101 30-31 -0.037337653099193346 1101 32-33 0.1474706331964697 1101 34-35 -0.0638374607422989 1101 36-37 0.1279090678618502 1101 38-39 0.26200423654626803 1101 40-41 0.22822573736704044 1101 42-43 0.10254978100878986 1101 44-45 0.6141751663381072 1101 46-47 0.16309578887261722 1101 48-49 0.1551144707095844 1101 50-51 0.20925909593038483 1101 52-53 0.16735938096257286 1101 54-55 0.37939056179014585 1101 56-57 0.40079642256799275 1101 58-59 0.5915901225558287 1101 60-61 0.3257031407705355 1101 62-63 0.452234886692942 1101 64-65 0.6769249138901685 1101 66-67 0.3466091286066373 1101 68-69 0.4943527905317495 1101 70-71 0.587383243975296 1101 72-73 0.9129642620507141 1101 74-75 0.6250923932460566 1101 76-77 0.7744833928188868 1101 78-79 0.991021670381663 1101 80-81 0.44230507687149156 1101 82-83 0.46754648131831544 1101 84-85 0.5775797548845034 1101 86-87 0.47719046427486234 1101 88-89 0.5187486853141081 1101 90-91 0.4294979385197024 1101 92-93 0.5231762914923834 1101 94-95 0.2373876428335535 1101 96-97 0.6339364510045478 1101 98-99 0.7127389911008741 1101 100-101 0.4998903196108131 2304 1 0.15690646208091152 2304 2 0.30023325150926894 2304 3 0.2055692556883031 2304 4 0.10114368125323381 2304 5 0.21612057109475558 2304 6 0.11349827646539268 2304 7 -0.038461317660676286 2304 8 0.08209681802027546 2304 9 -0.13077949082148876 2304 10-11 0.03217258644831844 2304 12-13 -0.042271044152592196 2304 14-15 -0.07992150846026647 2304 16-17 0.050783632828490965 2304 18-19 -0.010261882404222433 2304 20-21 -0.3276550201350048 2304 22-23 -0.12181417959659058 2304 24-25 -0.0768496028365604 2304 26-27 -0.22375171956861095 2304 28-29 -0.1117741342132419 2304 30-31 0.11666234690080302 2304 32-33 0.057999267557697465 2304 34-35 -0.1334233638260045 2304 36-37 0.01400598416140042 2304 38-39 0.103590139629965 2304 40-41 0.4345252968384088 2304 42-43 0.10432951669161383 2304 44-45 -0.35246800546806156 2304 46-47 -0.02468394681019248 2304 48-49 -0.007590375105401392 2304 50-51 0.1873912545206906 2304 52-53 0.019306517526452183 2304 54-55 -0.042309878738485907 2304 56-57 0.21221492477063464 2304 58-59 0.08695576132235061 2304 60-61 0.19774719363396542 2304 62-63 0.03816440211144112 2304 64-65 0.24786764516770177 2304 66-67 0.30383379821015666 2304 68-69 -0.005479808587189439 2304 70-71 -0.16329517012162853 2304 72-73 -0.07878023134134793 2304 74-75 0.2042641994134584 2304 76-77 -0.14233599044102618 2304 78-79 -0.29035277895754064 2304 80-81 -0.22922796277608626 2304 82-83 -0.06837422352750622 2304 84-85 0.006883719642210906 2304 86-87 -0.04875667228901648 2304 88-89 0.16045793641542616 2304 90-91 0.2070750310307119 2304 92-93 0.30300889061133063 2304 94-95 -0.5504978197215102 2304 96-97 -0.5949093639734251 2304 98-99 -0.6373667357713693 2304 100-101 -0.41582774206319684 2202 1 0.19978249513876278 2202 2 0.034481185393566705 2202 3 0.18791636312631965 2202 4 0.16299492092264956 2202 5 0.1369139595245059 2202 6 0.1553495161348124 2202 7 -0.022296028404475976 2202 8 0.10622904942523803 2202 9 0.10908827777355157 2202 10-11 0.007147793059893104 2202 12-13 0.01443969964905989 2202 14-15 0.028557830382709426 2202 16-17 0.11731255844832589 2202 18-19 0.16140753908338468 2202 20-21 0.2620639881294622 2202 22-23 0.2185659856926634 2202 24-25 -0.04676695820845822 2202 26-27 0.07077720605123261 2202 28-29 0.26632503934047946 2202 30-31 0.020513586570224618 2202 32-33 0.20163563119405836 2202 34-35 0.33771713204176734 2202 36-37 0.19076631473991768 2202 38-39 0.15383807351426526 2202 40-41 0.16518645386319974 2202 42-43 0.4382799299147493 2202 44-45 0.2607055482509466 2202 46-47 0.2715805159997231 2202 48-49 0.46374846786981294 2202 50-51 0.11071356857028292 2202 52-53 0.10471147620413745 2202 54-55 -0.17538425890377596 2202 56-57 0.17861161898550648 2202 58-59 0.5071706373554079 2202 60-61 0.3631025655347955 2202 62-63 0.5147098566568999 2202 64-65 0.008677562523075721 2202 66-67 0.11441231060685197 2202 68-69 0.12696647240453984 2202 70-71 -0.06955963293154355 2202 72-73 -0.18334221481242707 2202 74-75 -0.14909117248736692 2202 76-77 0.269349959972196 2202 78-79 -0.16468335746994 2202 80-81 -0.3992775495529486 2202 82-83 0.43031999134853294 2202 84-85 0.30402421550997616 2202 86-87 -0.09859138303281867 2202 88-89 0.19794553972121776 2202 90-91 0.03988494838608858 2202 92-93 -0.024726646578749012 2202 94-95 -0.08520856352316386 2202 96-97 0.10677658643979626 2202 98-99 -0.631565082878808 2202 100-101 -0.450422783385509 1102 1 0.2523483225460339 1102 2 0.16190767011391927 1102 3 0.3035227440603947 1102 4 0.2485545339664128 1102 5 0.19351591993196848 1102 6 0.07811843150415143 1102 7 0.24870147303700207 1102 8 0.32302705057841763 1102 9 0.1791119820467273 1102 10-11 0.3851028190064625 1102 12-13 0.52662818065361 1102 14-15 0.6535978713846902 1102 16-17 0.6376053382548506 1102 18-19 0.6736761020919033 1102 20-21 0.5843139721130584 1102 22-23 0.431007525829763 1102 24-25 0.6798170638301144 1102 26-27 0.7577366525244145 1102 28-29 0.4134196642363648 1102 30-31 0.5266158352728993 1102 32-33 0.37094500399180674 1102 34-35 0.6281425276468653 1102 36-37 0.5749517205955144 1102 38-39 0.6145978915679535 1102 40-41 0.6949128937376372 1102 42-43 0.8608566484745594 1102 44-45 0.696415715462166 1102 46-47 0.6993625648177115 1102 48-49 0.7789832683054598 1102 50-51 0.7011742002571282 1102 52-53 0.5167173702396255 1102 54-55 0.5631319817266274 1102 56-57 0.7146645371737392 1102 58-59 0.6152813427177009 1102 60-61 0.7752045579750515 1102 62-63 0.6938853323440028 1102 64-65 0.7784257847025913 1102 66-67 0.7082524028613264 1102 68-69 0.6319155402500201 1102 70-71 0.4232009539093866 1102 72-73 0.33906472989895775 1102 74-75 0.6306673001886551 1102 76-77 0.5780981180861104 1102 78-79 0.4832441202672584 1102 80-81 0.8973301767587998 1102 82-83 0.8281994198833544 1102 84-85 0.6700310064639154 1102 86-87 0.5910107695714473 1102 88-89 0.6582408821518584 1102 90-91 0.6642843333562922 1102 92-93 1.0232104409989304 1102 94-95 0.7107424903560045 1102 96-97 0.8883929616079733 1102 98-99 0.8281216363216508 1102 100-101 0.9499552036732375 2305 1 0.03178146208091448 2305 2 -0.08264174849072958 2305 3 0.208319255688302 2305 4 0.2770186812532316 2305 5 0.19415182109475637 2305 6 0.23421702646539444 2305 7 0.2616011823393265 2305 8 0.2583468180202786 2305 9 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sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 23.0 6 22.0 7 113.0 8 175.0 9 235.0 10 284.0 11 315.0 12 350.0 13 365.0 14 362.0 15 377.0 16 348.0 17 404.0 18 467.0 19 452.0 20 554.0 21 555.0 22 687.0 23 780.0 24 948.0 25 1161.0 26 1453.0 27 1810.0 28 2410.0 29 3009.0 30 3775.0 31 5212.0 32 7289.0 33 10021.0 34 14418.0 35 21824.0 36 37565.0 37 84592.0 38 62946.0 39 203.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.49143171358196 25.23633405394819 9.108747561333866 28.163486671135985 2 11.690820892149626 22.301857515177993 42.959369680169935 23.047951912502448 3 12.795839045481253 33.67781979240422 34.29623826812696 19.230102893987556 4 8.790430708507209 24.77892104430618 45.83640910528932 20.594239141897287 5 8.512737845940611 36.90397276164937 35.59403106497733 18.989258327432694 6 19.211689060151652 41.05409467415502 27.714056479043542 12.02015978664979 7 19.344971256790252 35.53270999871918 31.43670843159267 13.685610312897904 8 19.77094203854051 36.72311488688379 30.45905777308946 13.046885301486238 9 22.141519776075828 24.681763214502332 27.309783120547603 25.86693388887424 10-11 21.683769570393142 24.60068110242153 36.733571417808115 16.981977909377214 12-13 21.07275796072758 27.088513645885136 32.48828307488283 19.350445318504452 14-15 19.635248510968538 27.34543646234003 27.11581858115363 25.903496445537805 16-17 21.065340186729298 30.742905360227073 27.818973911765983 20.372780541277645 18-19 20.58860646242807 31.67682209016509 29.347258977425767 18.387312469981072 20-21 21.586583466276906 28.90001826993165 27.172662155060866 22.34073610873058 22-23 20.667025809295264 29.520770624644467 30.685786616537385 19.12641694952288 24-25 18.223638850270703 32.0497025457861 30.379513147137168 19.347145456806032 26-27 16.517003573453593 35.00515202718613 27.92775482755308 20.550089571807202 28-29 16.26671689583726 31.474288943303826 32.25503861367489 20.003955547184027 30-31 16.906791233836564 34.007967527147045 31.72997052203313 17.355270716983267 32-33 17.624474878963134 32.370815515325056 33.59286400542547 16.41184560028634 34-35 18.668085867957764 31.014006763184916 33.23191695788552 17.085990410971807 36-37 20.712802716013098 29.28832770009307 33.215958460976154 16.782911122917678 38-39 19.66078129945291 31.416821148136425 29.94717487302377 18.975222679386896 40-41 19.9210971044446 30.251762032785773 28.301986325758932 21.525154537010696 42-43 20.982348918308404 28.819549764595724 29.734979436273175 20.463121880822698 44-45 20.171363333220295 29.760249584590863 29.702035787355737 20.366351294833102 46-47 20.51785398055033 29.513796000798788 29.52717188577371 20.441178132877173 48-49 20.53040893145792 30.578675880466772 28.95621309801469 19.934702090060625 50-51 18.995674323265206 30.729260866943992 29.704173449086635 20.570891360704167 52-53 16.317998726407453 34.29863861246237 28.80448093567604 20.57888172545414 54-55 16.856002803305188 31.812050444421835 31.52606810072306 19.805878651549918 56-57 15.615810226046067 32.780353452482096 32.91392013503611 18.689916186435727 58-59 16.290867988508452 33.1576319879433 33.88159939716479 16.66990062638346 60-61 19.837966670120867 30.877335116956033 31.996307147365542 17.288391065557555 62-63 20.116103358744684 26.181853810006515 34.450779486522435 19.25126334472636 64-65 19.872556981009783 29.523671353771192 31.00686391067684 19.596907754542183 66-67 17.821328841305554 30.27728473501935 30.40710886007258 21.494277563602516 68-69 19.928364980612194 27.875669340889992 29.152739728608413 23.0432259498894 70-71 20.938825422486516 28.824800697910604 29.680796341621964 20.55557753798092 72-73 19.327777013569204 29.641389368874687 29.665697482696956 21.365136134859156 74-75 19.842017592125 29.42290443421043 29.142892675977777 21.592185297686793 76-77 18.087093193523888 28.450681929114047 31.18065986088156 22.281565016480503 78-79 16.56438481090889 33.01910789620951 28.788652460888258 21.627854831993336 80-81 16.738270209157715 30.663086489542113 31.34369700395704 21.254946297343132 82-83 17.08629908323945 30.82753371440629 32.325323767573124 19.760843434781133 84-85 17.4412054507535 31.229170159894043 32.04606726966836 19.28355711968409 86-87 18.01930128009466 29.987639945585613 32.75697043007714 19.23608834424259 88-89 21.10962262053476 25.534396379387985 32.62300302031696 20.732977979760296 90-91 21.123236464488123 28.487127698058607 29.596308633787533 20.793327203665743 92-93 18.113380342733958 27.55929252233952 31.723619397219384 22.603707737707143 94-95 20.437996095310602 25.183550553666866 30.7505596972735 23.62789365374903 96-97 21.628095435528248 28.3387358184765 28.730164712977274 21.303004033017977 98-99 19.94892622572347 28.852159327560052 29.273942009592822 21.92497243712366 100-101 20.90797550637313 28.214788526669523 29.60607779685518 21.27115817010217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.5 2 4.0 3 11.0 4 15.5 5 15.5 6 20.5 7 23.5 8 29.0 9 36.0 10 42.0 11 44.0 12 43.0 13 52.0 14 59.5 15 70.5 16 91.5 17 115.5 18 171.0 19 277.0 20 428.0 21 659.0 22 1008.0 23 1648.5 24 2484.5 25 3544.5 26 4904.5 27 5734.0 28 6159.0 29 7067.0 30 7928.0 31 8969.5 32 9862.0 33 9424.0 34 8681.0 35 8574.5 36 10449.5 37 11442.5 38 10585.0 39 10582.5 40 10889.0 41 10676.0 42 10390.0 43 11034.0 44 11908.5 45 12429.5 46 11898.5 47 10052.0 48 7765.0 49 6035.0 50 5108.5 51 4781.0 52 4234.0 53 3308.0 54 2523.0 55 1957.5 56 1572.5 57 1256.0 58 1043.0 59 992.5 60 969.5 61 774.5 62 554.0 63 410.5 64 324.0 65 268.5 66 223.0 67 187.5 68 160.5 69 122.5 70 91.0 71 72.5 72 55.5 73 44.0 74 35.0 75 23.5 76 16.0 77 17.0 78 13.5 79 8.0 80 6.0 81 5.5 82 3.5 83 3.0 84 2.5 85 1.5 86 1.5 87 1.5 88 2.0 89 2.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.532502749363503E-4 2 0.0 3 0.0 4 0.0 5 0.003013001099745401 6 0.01393513008632248 7 0.02335075852302686 8 0.03050663613492219 9 0.025987134485304087 10-11 0.02410400879796321 12-13 0.033143012097199415 14-15 0.028246885310113136 16-17 0.01939619457961102 18-19 0.022032570541888247 20-21 0.0197728197170792 22-23 0.025987134485304087 24-25 0.03653263833441299 26-27 0.03257807439099715 28-29 0.024857259072899564 30-31 0.023915696229229122 32-33 0.03803913888428569 34-35 0.04086382741529701 36-37 0.047831392458458244 38-39 0.04218201539643562 40-41 0.04764307988972416 42-43 0.04575995420238328 44-45 0.04331189080884014 46-47 0.04255864053390379 48-49 0.044065141083776495 50-51 0.04670151704605372 52-53 0.047078142183521896 54-55 0.04331189080884014 56-57 0.03973395200289248 58-59 0.03898070172795613 60-61 0.052162581539342265 62-63 0.056117145482758105 64-65 0.05366908208921496 66-67 0.059130146582503505 68-69 0.05385739465794905 70-71 0.05743533346389671 72-73 0.06496783621326022 74-75 0.06402627336958978 76-77 0.07626659033730547 78-79 0.058188583738833066 80-81 0.06251977281971709 82-83 0.047078142183521896 84-85 0.04575995420238328 86-87 0.05461064493288541 88-89 0.055175582639087666 90-91 0.05272751924554452 92-93 0.06741589960680336 94-95 0.07306527666882598 96-97 0.07758477831844408 98-99 0.0804094668494554 100-101 0.08379909308666898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265516.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.403392258893795 #Duplication Level Percentage of deduplicated Percentage of total 1 82.47731070731386 48.99432040405226 2 9.087817306940007 10.796943525226421 3 3.0203607650649418 5.382590258715759 4 1.4179826977776377 3.3693192964963785 5 0.9053933600180928 2.68917184568763 6 0.577182588450877 2.057196222405066 7 0.4823675329298541 2.0057987440110896 8 0.32910750928762766 1.564008197564838 9 0.2950411210570284 1.5773799101988981 >10 1.2880847180901382 13.938343862597039 >50 0.08386933392340878 3.319608152968126 >100 0.03292263595601946 3.4127494470651034 >500 0.0025597231904497977 0.8925701330114537 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT 659 0.24819596559152746 No Hit CTTTATAAATTCATCTAATGGTCTAACTTTGGGAATTCATCTAATGGTCT 590 0.22220883110622333 No Hit CTTTATAAATTCATCTAATGGTCTAACTTTATAAATTCATCTAATGGTCT 570 0.21467632835685985 No Hit CTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT 529 0.19923469772066466 No Hit ATTCCCAAAGTTAGACCATTAGATGAATTTATAAAGTTAGACCATTAGAT 426 0.16044230856144262 No Hit GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC 402 0.1514033052622064 No Hit GTAATTTATTTGGTGAAGCCAGAGAGTAAAATAATTGGTGAAGCCAGAGA 318 0.11976679371487971 No Hit GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG 295 0.11110441555311167 No Hit CTATTTTTGGTGAACGGCCAGAGTCACTATTTTTGGTGAACGGCCAGAGT 290 0.10922128986577082 No Hit GTTTCAATTGTGCACTGGTTTTCAAATTTTTTTAAACTGAGAATTATTGA 283 0.10658491390349357 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0011298754124045257 20-21 0.0 0.0 0.0026363759622772265 22-23 0.0 0.0 0.005649377062022627 24-25 0.0 0.0 0.009980566142906643 26-27 0.0 0.0 0.01638319347986562 28-29 0.0 0.0 0.03803913888428569 30-31 0.0 0.0 0.059883396857439854 32-33 0.0 0.0 0.08304584281173263 34-35 0.0 0.0 0.12993567242652043 36-37 0.0 0.0 0.234260835505205 38-39 0.0 0.0 0.4574112294550987 40-41 0.0 0.0 0.7941141023516474 42-43 0.0 1.883125687340876E-4 1.1690444267012157 44-45 0.0 3.766251374681752E-4 1.6270205938625169 46-47 0.0 3.766251374681752E-4 2.1642763524608686 48-49 0.0 3.766251374681752E-4 2.7440907515931245 50-51 0.0 3.766251374681752E-4 3.437834254809503 52-53 0.0 3.766251374681752E-4 4.106908811521716 54-55 0.0 3.766251374681752E-4 4.785022371533165 56-57 0.0 3.766251374681752E-4 5.54674671206255 58-59 0.0 3.766251374681752E-4 6.32673737175914 60-61 0.0 3.766251374681752E-4 7.229319513701622 62-63 0.0 3.766251374681752E-4 8.325863601440215 64-65 0.0 3.766251374681752E-4 9.480407960348906 66-67 0.0 3.766251374681752E-4 10.25644405610208 68-69 0.0 3.766251374681752E-4 11.068447852483466 70-71 0.0 3.766251374681752E-4 12.14107624399283 72-73 0.0 3.766251374681752E-4 12.910333087271576 74-75 0.0 3.766251374681752E-4 13.655485921752362 76-77 0.0 3.766251374681752E-4 14.426249265580982 78-79 0.0 3.766251374681752E-4 15.1461682158514 80-81 0.0 3.766251374681752E-4 15.863262477590805 82-83 0.0 3.766251374681752E-4 16.56604498410642 84-85 0.0 3.766251374681752E-4 17.294249687401134 86-87 0.0 3.766251374681752E-4 18.12828605432441 88-89 0.0 3.766251374681752E-4 19.16438180749936 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 20 0.002074663 71.22966 8 TAGACCG 20 0.002076214 71.21624 5 ACCGTAC 25 0.005022708 56.983727 9 AGGAGTG 25 0.0050264546 56.972992 5 GCGCCGG 25 0.0050264546 56.972992 6 TTCATGC 25 0.0050264546 56.972992 3 ACGACCT 25 0.0050264546 56.972992 5 AGACCGT 40 5.3932366E-4 47.477497 6 TGAGTCA 60 8.296661E-5 39.564575 5 GTAGAGG 100 3.0284355E-8 37.981995 1 TATAGGA 50 0.0016173392 37.981995 2 GCATCTA 75 6.924689E-6 37.981995 1 GGTAGTA 100 3.0284355E-8 37.981995 2 GAGTGTG 65 1.3276626E-4 36.52803 7 CACCTAT 55 0.0025820627 34.529087 1 CCTAACA 85 1.6327853E-5 33.513527 1 ATCTGGT 85 1.6327853E-5 33.513527 5 GTACACT 115 1.03558705E-7 33.027824 6 CCGGACA 60 0.0039510475 31.657625 9 TAGAGGT 90 2.4138366E-5 31.651663 2 >>END_MODULE