FastQCFastQC Report
Wed 17 Sep 2014
G50_Mov_M1_L36_GCTACGCT-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_Mov_M1_L36_GCTACGCT-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265516
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT6590.24819596559152746No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTGGGAATTCATCTAATGGTCT5900.22220883110622333No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTATAAATTCATCTAATGGTCT5700.21467632835685985No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT5290.19923469772066466No Hit
ATTCCCAAAGTTAGACCATTAGATGAATTTATAAAGTTAGACCATTAGAT4260.16044230856144262No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC4020.1514033052622064No Hit
GTAATTTATTTGGTGAAGCCAGAGAGTAAAATAATTGGTGAAGCCAGAGA3180.11976679371487971No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG2950.11110441555311167No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTATTTTTGGTGAACGGCCAGAGT2900.10922128986577082No Hit
GTTTCAATTGTGCACTGGTTTTCAAATTTTTTTAAACTGAGAATTATTGA2830.10658491390349357No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG200.00207466371.229668
TAGACCG200.00207621471.216245
ACCGTAC250.00502270856.9837279
AGGAGTG250.005026454656.9729925
GCGCCGG250.005026454656.9729926
TTCATGC250.005026454656.9729923
ACGACCT250.005026454656.9729925
AGACCGT405.3932366E-447.4774976
TGAGTCA608.296661E-539.5645755
GTAGAGG1003.0284355E-837.9819951
TATAGGA500.001617339237.9819952
GCATCTA756.924689E-637.9819951
GGTAGTA1003.0284355E-837.9819952
GAGTGTG651.3276626E-436.528037
CACCTAT550.002582062734.5290871
CCTAACA851.6327853E-533.5135271
ATCTGGT851.6327853E-533.5135275
GTACACT1151.03558705E-733.0278246
CCGGACA600.003951047531.6576259
TAGAGGT902.4138366E-531.6516632
Produced by FastQC (version 0.11.2)