##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G50_Mov_M1_L36_GCTACGCT-AAGGAGTA_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 265516 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79272058934301 33.0 33.0 33.0 33.0 33.0 2 32.83020232302385 33.0 33.0 33.0 33.0 33.0 3 32.84034107172449 33.0 33.0 33.0 33.0 33.0 4 36.81279470917007 37.0 37.0 37.0 37.0 37.0 5 36.74547673209901 37.0 37.0 37.0 37.0 37.0 6 36.742866719896355 37.0 37.0 37.0 37.0 37.0 7 36.71326775034273 37.0 37.0 37.0 37.0 37.0 8 36.7044509558746 37.0 37.0 37.0 37.0 37.0 9 36.72632911011012 37.0 37.0 37.0 37.0 37.0 10-11 36.69608987782281 37.0 37.0 37.0 37.0 37.0 12-13 36.69328402054867 37.0 37.0 37.0 37.0 37.0 14-15 39.09337290408111 40.0 40.0 40.0 37.0 40.0 16-17 39.058009686798535 40.0 40.0 40.0 37.0 40.0 18-19 38.9914317781226 40.0 40.0 40.0 37.0 40.0 20-21 38.981368354449444 40.0 40.0 40.0 37.0 40.0 22-23 38.86793074616972 40.0 38.5 40.0 37.0 40.0 24-25 38.80823377875533 40.0 37.0 40.0 37.0 40.0 26-27 38.80163718947257 40.0 37.0 40.0 37.0 40.0 28-29 38.77573667876889 40.0 37.0 40.0 37.0 40.0 30-31 38.70874636556742 40.0 37.0 40.0 37.0 40.0 32-33 38.62274213230088 40.0 37.0 40.0 37.0 40.0 34-35 38.5242188794649 40.0 37.0 40.0 37.0 40.0 36-37 38.389803251028184 40.0 37.0 40.0 37.0 40.0 38-39 38.322677352777234 40.0 37.0 40.0 37.0 40.0 40-41 38.30105530363518 40.0 37.0 40.0 37.0 40.0 42-43 38.19382636074662 40.0 37.0 40.0 37.0 40.0 44-45 38.02102133204779 40.0 37.0 40.0 37.0 40.0 46-47 37.92977071061631 40.0 37.0 40.0 35.0 40.0 48-49 37.855351466578284 40.0 37.0 40.0 35.0 40.0 50-51 38.0957983699664 40.0 37.0 40.0 37.0 40.0 52-53 38.10397113544946 40.0 37.0 40.0 37.0 40.0 54-55 37.97619540818633 40.0 37.0 40.0 37.0 40.0 56-57 37.76388240256708 40.0 37.0 40.0 33.0 40.0 58-59 37.60045722291689 40.0 37.0 40.0 33.0 40.0 60-61 37.43020947890146 38.5 37.0 40.0 33.0 40.0 62-63 37.13541180192531 37.0 37.0 40.0 33.0 40.0 64-65 36.94833832989349 37.0 37.0 40.0 33.0 40.0 66-67 36.747949276126484 37.0 37.0 40.0 33.0 40.0 68-69 36.511394793534095 37.0 37.0 40.0 33.0 40.0 70-71 36.162206797330484 37.0 37.0 38.5 33.0 40.0 72-73 35.870512511487064 37.0 37.0 37.0 33.0 40.0 74-75 35.629815152382534 37.0 37.0 37.0 33.0 40.0 76-77 34.42168080266349 37.0 33.0 37.0 30.0 37.0 78-79 35.26353590744061 37.0 33.0 37.0 33.0 37.0 80-81 35.226645475225595 37.0 33.0 37.0 33.0 37.0 82-83 34.97216928546679 37.0 33.0 37.0 33.0 37.0 84-85 34.82962796968921 37.0 33.0 37.0 33.0 37.0 86-87 34.72196590789255 37.0 33.0 37.0 33.0 37.0 88-89 34.5476619111466 37.0 33.0 37.0 30.0 37.0 90-91 34.41941351933593 37.0 33.0 37.0 27.0 37.0 92-93 34.39432086955212 37.0 33.0 37.0 27.0 37.0 94-95 34.315077057503125 37.0 33.0 37.0 27.0 37.0 96-97 34.06083625845524 37.0 33.0 37.0 27.0 37.0 98-99 33.961077675168355 37.0 33.0 37.0 27.0 37.0 100-101 33.13241386583106 35.0 33.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.05854227352099883 2306 2 0.056930641396647275 2306 3 -0.002717783374968974 2306 4 -0.0281407532577731 2306 5 0.022263190842778613 2306 6 -0.02135140913746625 2306 7 -0.014929645303325856 2306 8 0.039060702046342044 2306 9 0.10662511444459 2306 10-11 -0.010814313458574532 2306 12-13 0.09136447274543258 2306 14-15 -0.10347662310203276 2306 16-17 0.090042537535858 2306 18-19 -0.09421422111323352 2306 20-21 -0.10778736610806305 2306 22-23 0.21854277486725238 2306 24-25 0.19152461687389888 2306 26-27 0.11920670463667449 2306 28-29 0.05334736338994617 2306 30-31 0.1694906938898697 2306 32-33 0.2820892776848467 2306 34-35 -0.0024483326232669356 2306 36-37 -0.14811494968774497 2306 38-39 0.037033712927005524 2306 40-41 0.24048019617646332 2306 42-43 0.3409508958558263 2306 44-45 0.10853662535551933 2306 46-47 -0.08316941083339202 2306 48-49 -0.2782302733841888 2306 50-51 -0.009891108097590973 2306 52-53 0.08876189502497311 2306 54-55 0.08733028388993347 2306 56-57 -0.019717572230490532 2306 58-59 -0.0064521601581688515 2306 60-61 0.13379405633660468 2306 62-63 0.16870829349291938 2306 64-65 0.20190287605831259 2306 66-67 0.28904086928408645 2306 68-69 0.49651753710683266 2306 70-71 0.16673680910082567 2306 72-73 0.1054812549064934 2306 74-75 0.24864545400995297 2306 76-77 0.24723717298309822 2306 78-79 0.24602045010103524 2306 80-81 -0.11735675491578235 2306 82-83 0.1817700104051596 2306 84-85 0.49720723237179953 2306 86-87 0.17130401975953902 2306 88-89 0.20408778024236796 2306 90-91 0.31182401725668996 2306 92-93 0.16660946869669857 2306 94-95 0.08071880311047863 2306 96-97 0.22733315330187054 2306 98-99 -0.07620913881050484 2306 100-101 0.3945085301950684 2307 1 -0.17363030361380538 2307 2 -0.029741999750456216 2307 3 0.13362839509129998 2307 4 0.08866033921864869 2307 5 0.15732912350185302 2307 6 0.08439689453683741 2307 7 -0.031103417334222172 2307 8 0.154794923016766 2307 9 0.08451953684035374 2307 10-11 0.08739120866834327 2307 12-13 0.09861919898620641 2307 14-15 0.2942530743332128 2307 16-17 0.2630677583782557 2307 18-19 0.23394478541601416 2307 20-21 0.31802238936499094 2307 22-23 0.31753778190860515 2307 24-25 0.386623409175364 2307 26-27 0.4011168313810103 2307 28-29 0.33975968788958255 2307 30-31 0.26309799982594484 2307 32-33 0.29514778491823535 2307 34-35 0.2238244450833804 2307 36-37 0.12496148098693993 2307 38-39 0.26849148615280427 2307 40-41 0.34796750040554514 2307 42-43 0.2634853558054644 2307 44-45 0.5709614109143502 2307 46-47 0.5523872827184348 2307 48-49 0.4959983785169797 2307 50-51 0.3855128094545819 2307 52-53 0.3180219129484243 2307 54-55 0.22513807631940352 2307 56-57 0.18586856697490362 2307 58-59 0.18180798578985247 2307 60-61 0.07853011232602114 2307 62-63 0.19094189567787367 2307 64-65 0.28380660157360893 2307 66-67 0.23483312806443735 2307 68-69 0.2310692377000052 2307 70-71 0.3110525177168739 2307 72-73 0.0635852108660373 2307 74-75 0.32828741193253563 2307 76-77 0.5112238584267104 2307 78-79 0.3083150773361254 2307 80-81 0.18756579021288644 2307 82-83 0.3802827915258007 2307 84-85 0.2984384020897224 2307 86-87 0.008915507831915193 2307 88-89 0.281969400180067 2307 90-91 0.40281748800307327 2307 92-93 0.3256586728105546 2307 94-95 0.6513883716676432 2307 96-97 0.4264967260411723 2307 98-99 0.39802591431969603 2307 100-101 0.8539460755371024 2201 1 0.11588255719175322 2201 2 0.13997100992616396 2201 3 -0.019750150934669364 2201 4 0.15507337370711127 2201 5 0.12639702524695196 2201 6 0.10217871572615422 2201 7 0.10916620469869542 2201 8 0.1528926816802354 2201 9 0.15539870316241178 2201 10-11 0.08023712712007836 2201 12-13 0.1613931630917378 2201 14-15 0.311745424014795 2201 16-17 0.2473949438881391 2201 18-19 0.17075729058515066 2201 20-21 0.28171439168075807 2201 22-23 0.36916709296642125 2201 24-25 0.13687152761055899 2201 26-27 0.11141788174905543 2201 28-29 -0.017489505725507115 2201 30-31 0.059558176817091635 2201 32-33 0.01787543358253174 2201 34-35 0.19847501162048076 2201 36-37 0.14164744575412413 2201 38-39 0.19701270027901074 2201 40-41 0.436483752163042 2201 42-43 0.07727247018807049 2201 44-45 0.41675580343771657 2201 46-47 0.09686251222799314 2201 48-49 0.05823475267958145 2201 50-51 0.021688477306703646 2201 52-53 -0.08374386324874905 2201 54-55 0.12190013074005179 2201 56-57 0.267478855619359 2201 58-59 0.5683144782222413 2201 60-61 0.3973209484851523 2201 62-63 0.03392427926897312 2201 64-65 0.036246432853069166 2201 66-67 0.0033799810956125498 2201 68-69 0.07938616764790396 2201 70-71 0.21701563077617436 2201 72-73 0.13845494868755281 2201 74-75 0.13864222129487302 2201 76-77 0.20347429263397032 2201 78-79 0.23954491771326758 2201 80-81 0.4753211454357782 2201 82-83 0.348254836848767 2201 84-85 0.5182299826041472 2201 86-87 0.2281694984034175 2201 88-89 0.28889603982939605 2201 90-91 0.11686099143536666 2201 92-93 0.09470176271213404 2201 94-95 0.038804631695761316 2201 96-97 0.46729153209525975 2201 98-99 0.19024740137618323 2201 100-101 0.10713026212216903 1101 1 -0.011519883258912955 1101 2 -0.04052877565724344 1101 3 0.02950718796049756 1101 4 -0.03868125574203418 1101 5 0.021176955853945856 1101 6 0.16451546673000905 1101 7 0.04823290775121336 1101 8 0.021318863895352536 1101 9 0.10296410022295532 1101 10-11 0.1053725994213579 1101 12-13 0.10905602223952826 1101 14-15 0.12038836191195657 1101 16-17 0.1863252000185085 1101 18-19 0.1874525461307428 1101 20-21 0.3287885798215697 1101 22-23 0.2621732135854984 1101 24-25 0.22940203324386488 1101 26-27 0.17107442535358786 1101 28-29 0.1618602272283809 1101 30-31 0.17058698661008975 1101 32-33 0.28133494783148194 1101 34-35 0.1509622440870544 1101 36-37 0.14350897162055531 1101 38-39 0.23416524793797322 1101 40-41 0.08275485270493732 1101 42-43 0.24546279415204708 1101 44-45 0.38371615586062546 1101 46-47 0.2506869245315997 1101 48-49 0.08037813890233991 1101 50-51 0.14655690348891426 1101 52-53 0.01960493406909336 1101 54-55 0.09268099698308419 1101 56-57 0.2847198988535382 1101 58-59 0.0827096285087876 1101 60-61 0.2083419613111559 1101 62-63 0.47790307151878864 1101 64-65 0.46778906277003784 1101 66-67 0.11662118026914214 1101 68-69 0.655942637181262 1101 70-71 0.4882623657962526 1101 72-73 0.4532574904987925 1101 74-75 0.5051678928086574 1101 76-77 0.2226158465694894 1101 78-79 0.21725533835964228 1101 80-81 0.44781734441437493 1101 82-83 0.5470969872522602 1101 84-85 0.6023105252730545 1101 86-87 0.36470413642922495 1101 88-89 0.4081993808297426 1101 90-91 0.6054091961837216 1101 92-93 0.4183343696280417 1101 94-95 0.44020223802689173 1101 96-97 0.3899163810290247 1101 98-99 0.13225745946358103 1101 100-101 0.6389516336086629 2304 1 0.09952857489086853 2304 2 0.04651968249253713 2304 3 0.11366577826886726 2304 4 0.10877248875135592 2304 5 0.07344127303456105 2304 6 0.1486211936022599 2304 7 0.15730779761905467 2304 8 0.17763604451209858 2304 9 0.16474383590577446 2304 10-11 0.19798933950946207 2304 12-13 0.12686218964041274 2304 14-15 0.245692326669662 2304 16-17 0.2325630854810612 2304 18-19 0.1285802994347094 2304 20-21 0.17755509964536031 2304 22-23 -0.012443526502607938 2304 24-25 0.06929630637161921 2304 26-27 0.13945327997914347 2304 28-29 0.28689987480547074 2304 30-31 0.16109799982594097 2304 32-33 0.06118516809580399 2304 34-35 -0.08692321846801576 2304 36-37 0.13021381743553917 2304 38-39 0.07064101886307839 2304 40-41 0.38602357517189034 2304 42-43 0.21247601001108052 2304 44-45 0.07275580343771537 2304 46-47 -0.08899589485165649 2304 48-49 -0.12321657475404635 2304 50-51 -0.045178779330463215 2304 52-53 -0.11156687209830807 2304 54-55 0.1011567679081935 2304 56-57 0.2111956697786468 2304 58-59 0.19001359326649236 2304 60-61 -0.02890914001042688 2304 62-63 -0.0728898800230624 2304 64-65 -0.11557657599647797 2304 66-67 -0.3908678065150042 2304 68-69 -0.024295248281298143 2304 70-71 0.03602448033369399 2304 72-73 0.07863193983800443 2304 74-75 0.011969654923191797 2304 76-77 -0.013392964003195118 2304 78-79 -0.0381895955610716 2304 80-81 -0.06404168642262675 2304 82-83 0.1254229784416907 2304 84-85 -0.17548683155514055 2304 86-87 -0.3184676697381761 2304 88-89 -0.43724555309096047 2304 90-91 -0.18469653068851954 2304 92-93 -0.06269646737636236 2304 94-95 -0.3079387311360975 2304 96-97 -0.37086775994013976 2304 98-99 -0.33546941278310527 2304 100-101 -0.7834184104442059 2202 1 0.12114840960160933 2202 2 0.14172629406278503 2202 3 0.11207900140936289 2202 4 0.09426009205714081 2202 5 0.05962309121638043 2202 6 0.14518317707333495 2202 7 -0.020212863537970804 2202 8 0.145140176743503 2202 9 -0.13568591616034098 2202 10-11 0.0525182651293008 2202 12-13 0.107308470632141 2202 14-15 0.06584108700024416 2202 16-17 0.004397796224864692 2202 18-19 0.3655389771206643 2202 20-21 0.24648071948006844 2202 22-23 0.19932506853871956 2202 24-25 0.10617233942947024 2202 26-27 0.1575855113840916 2202 28-29 0.0027511144748899596 2202 30-31 0.20111452875156033 2202 32-33 0.28806120115365275 2202 34-35 0.11555612037496132 2202 36-37 0.38652786702231623 2202 38-39 0.1347732502680401 2202 40-41 -0.05419130086116297 2202 42-43 -0.0059206842038008745 2202 44-45 0.4291524976525949 2202 46-47 0.3889710472970975 2202 48-49 0.25213879714677745 2202 50-51 0.17673857604143706 2202 52-53 -0.0698478638338429 2202 54-55 0.11725594146191298 2202 56-57 0.19979071110095958 2202 58-59 0.14386483293591112 2202 60-61 0.24495862858461237 2202 62-63 0.315044004274462 2202 64-65 0.15758044879689947 2202 66-67 -0.0945868147794684 2202 68-69 -0.12907210778542577 2202 70-71 0.07101621587088403 2202 72-73 -0.06872343206282494 2202 74-75 0.08170519211327587 2202 76-77 0.23868968062490126 2202 78-79 -0.1590325707676783 2202 80-81 -0.27837226493502243 2202 82-83 -0.3182960298227755 2202 84-85 0.08591812712254665 2202 86-87 0.4116149748899218 2202 88-89 -0.43296456135542627 2202 90-91 -0.17277917531662013 2202 92-93 -0.13697745911189685 2202 94-95 -0.08980649973113941 2202 96-97 -0.14645453679963794 2202 98-99 0.08851405829127401 2202 100-101 0.21487911021695538 1102 1 -0.01764971968277962 1102 2 -0.019526829135372736 1102 3 0.1151541503618958 1102 4 0.11075698487538688 1102 5 0.1174102652826221 1102 6 0.18285375174179563 1102 7 0.14552485188261954 1102 8 0.20811666466713774 1102 9 0.08995313823135831 1102 10-11 0.14257848679628893 1102 12-13 0.09869164700474897 1102 14-15 0.19927372201849636 1102 16-17 0.1190592095120735 1102 18-19 0.15825471803935898 1102 20-21 0.2117411461569887 1102 22-23 0.3238045355128989 1102 24-25 0.24207150017007706 1102 26-27 0.3143059931574399 1102 28-29 0.17745801434035968 1102 30-31 0.09061737967090266 1102 32-33 0.29992935414231425 1102 34-35 0.2522240683536836 1102 36-37 0.06808978642778385 1102 38-39 0.3496332669250961 1102 40-41 0.3125041953269374 1102 42-43 0.10531321931340187 1102 44-45 0.01946898173228817 1102 46-47 0.17004286483825837 1102 48-49 0.3483493167188172 1102 50-51 0.1613948640803926 1102 52-53 0.5682935930179696 1102 54-55 0.15194746558261585 1102 56-57 0.043598770553842314 1102 58-59 0.16740894210370527 1102 60-61 0.20064124758646784 1102 62-63 0.1019938409071699 1102 64-65 0.4363459046236713 1102 66-67 0.18587637953150704 1102 68-69 0.07204583699002143 1102 70-71 -0.04330885299962972 1102 72-73 -0.03508899039455571 1102 74-75 0.4136130657759054 1102 76-77 0.5738008344464092 1102 78-79 0.5615933501753645 1102 80-81 0.6447490112517897 1102 82-83 0.6695315055734739 1102 84-85 0.5134278971270305 1102 86-87 0.6091292294866264 1102 88-89 0.5393590980718272 1102 90-91 0.525055407295973 1102 92-93 0.7550399667321628 1102 94-95 0.5572240595615767 1102 96-97 0.6860779764939764 1102 98-99 0.5420344631858782 1102 100-101 0.38872112443952034 2305 1 0.12521607489086506 2305 2 0.006363432492534571 2305 3 0.11479077826886908 2305 4 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-0.5882237302650921 1213 74-75 -0.7102880770355711 1213 76-77 -1.1253620361681484 1213 78-79 -0.619158667726019 1213 80-81 -0.5953612740514913 1213 82-83 -0.484937846300582 1213 84-85 -1.89033219237988 1213 86-87 -1.2114573604598249 1213 88-89 -1.8341424603074543 1213 90-91 -2.1463563245029533 1213 92-93 -1.608222240572239 1213 94-95 -1.131309865156716 1213 96-97 -1.5775378630329229 1213 98-99 -1.0482116808243447 1213 100-101 -0.7763771733307934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 7.0 16 27.0 17 39.0 18 66.0 19 107.0 20 165.0 21 240.0 22 321.0 23 417.0 24 560.0 25 718.0 26 920.0 27 1216.0 28 1488.0 29 1966.0 30 2532.0 31 3337.0 32 4595.0 33 6618.0 34 10286.0 35 17221.0 36 34408.0 37 91119.0 38 86779.0 39 361.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.956431621274305 24.94337731248563 9.250696242363945 28.84949482387612 2 11.510677563933562 21.62894052954691 43.51097887085233 23.34940303566721 3 12.523728456068458 33.02210136193805 34.98139387730505 19.47277630468844 4 8.726781060275087 24.36275026740385 46.060877687220355 20.84959098510071 5 8.255999638439867 36.03699965350488 35.95489537353681 19.752105334518447 6 18.9171274047515 40.60885219723105 28.257807439099714 12.21621295891773 7 18.99358230765754 35.40803567393302 31.843278747796745 13.755103270612693 8 19.600325404118774 36.12061043402281 31.219587520149446 13.059476641708976 9 21.703776796878532 24.850103195287666 27.35466035945103 26.09145964838277 10-11 21.363347902476505 23.94379989115513 37.45694695789888 17.23590524846949 12-13 20.753551575046323 26.500286235104475 33.02964793082149 19.716514259027704 14-15 19.44816884858163 27.085750011298753 27.550317118365747 25.91576402175387 16-17 21.14599496828816 30.060900284728604 28.251216499194022 20.54188824778921 18-19 20.551303876225916 31.00095662784917 29.958834872474725 18.488904623450185 20-21 21.429028759095498 28.520880097621237 27.801714397625755 22.24837674565751 22-23 20.78330496090631 28.897693547658143 31.14878199430543 19.170219497130116 24-25 18.06953253287937 31.419575468145045 30.822436312689256 19.688455686286325 26-27 16.351745280887027 34.3467060365477 28.523328161014778 20.77822052155049 28-29 16.055906235405775 30.927891351180342 32.66281504692749 20.353387366486388 30-31 16.938150619924976 33.51888398439266 31.966246855180103 17.576718540502267 32-33 17.563724973259614 31.799025294144233 34.153873966164 16.483375766432154 34-35 18.662905436960482 30.61472754937555 33.57707256813149 17.14529444553247 36-37 20.37090043537866 28.866810286385753 33.698910800102446 17.063378478133146 38-39 19.45965591527441 30.834488317088237 30.50588288464725 19.199972882990103 40-41 19.782047032947165 29.886522846080837 28.557036110818178 21.774394010153813 42-43 20.936403079287125 28.301119329908552 29.96938037632384 20.793097214480483 44-45 20.210081501679745 28.939687250485846 30.339226261317588 20.51100498651682 46-47 20.241906325795807 29.13459075912563 30.14469937781527 20.47880353726329 48-49 20.51382967504783 30.06617303665316 29.57185254372618 19.84814474457283 50-51 18.99377062022628 30.130952560297686 30.10967324003073 20.765603579445308 52-53 16.09564018740867 33.59985838894831 29.39804004278462 20.906461380858403 54-55 16.801812320161496 31.169119751728708 31.9071167085976 20.121951219512198 56-57 15.50923484837072 32.431755525090765 33.11005739767095 18.94895222886756 58-59 16.144413142710796 32.52139230780819 34.33503065728619 16.999163892194822 60-61 19.68450112234291 30.38103165157655 32.29560553789602 17.638861688184516 62-63 19.705592130041126 25.764360716491662 34.95420238328387 19.57584477018334 64-65 19.212966450232756 29.0869476792359 31.50525768691906 20.194828183612287 66-67 17.318542008767835 29.888029346630713 30.687039575769443 22.106389068832012 68-69 19.604279968062187 27.545985929284868 29.266597869808226 23.583136232844726 70-71 20.723609876617605 28.256112625981107 29.88237996956869 21.137897527832596 72-73 19.07060214826978 29.362072342156402 29.649625634613358 21.917699874960455 74-75 19.49844830443363 29.07112942346224 29.222909353861915 22.207512918242216 76-77 17.809284562888866 28.259125627080856 30.959339550158937 22.972250259871345 78-79 16.28131638018048 33.05657662815047 28.10922882236852 22.552878169300534 80-81 16.43422618599256 30.256745356212058 31.048599707738894 22.260428750056494 82-83 16.438745687642175 30.641656246704528 31.674174061073536 21.24542400457976 84-85 17.23041172660028 31.19962638786363 31.186444508052247 20.38351737748384 86-87 17.809849500595067 30.091783546000993 31.821057864686118 20.277309088717818 88-89 20.27734727351134 25.776461261206972 31.743909489276522 22.202281976005168 90-91 20.236256948733786 28.70109522589976 28.937804124798504 22.12484370056795 92-93 17.59762123563175 27.651629280344686 30.977040931619943 23.773708552403622 94-95 19.527636752587416 25.512398499525453 29.562248602720743 25.39771614516639 96-97 20.913805571039035 28.327671402100062 27.886831678693564 22.871691348167342 98-99 19.18603775290378 28.652698895735096 28.745160366983534 23.41610298437759 100-101 19.719715572696185 28.555906235405775 29.232324982298618 22.492053209599423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 2.0 11 4.0 12 9.5 13 12.5 14 19.5 15 36.5 16 62.5 17 90.5 18 129.0 19 223.0 20 393.5 21 620.5 22 965.0 23 1551.5 24 2391.5 25 3457.5 26 4802.0 27 5675.0 28 6111.5 29 7075.5 30 7928.5 31 8953.0 32 9833.5 33 9349.0 34 8510.5 35 8368.0 36 10237.0 37 11141.0 38 10345.0 39 10340.0 40 10568.0 41 10446.5 42 10334.0 43 11111.0 44 11917.0 45 12380.5 46 11946.5 47 10224.0 48 8005.0 49 6278.5 50 5411.0 51 5104.5 52 4592.0 53 3713.0 54 2836.0 55 2167.0 56 1746.0 57 1442.0 58 1226.0 59 1107.0 60 1064.5 61 820.5 62 522.5 63 391.0 64 313.5 65 249.0 66 200.5 67 166.5 68 138.0 69 108.5 70 86.0 71 73.0 72 58.0 73 46.0 74 28.0 75 14.5 76 15.5 77 10.0 78 6.0 79 4.0 80 2.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06138989740731255 2 0.002259750824809051 3 0.004519501649618102 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.8831256873408757E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.8831256873408757E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 265516.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.52614156062721 #Duplication Level Percentage of deduplicated Percentage of total 1 82.32271492136641 48.18030867143048 2 9.168414216754352 10.731838166724643 3 3.047112752304388 5.35007256877677 4 1.455361541104589 3.40706782306319 5 0.9416831537755987 2.7556540781564287 6 0.5719999993959606 2.0086171762396 7 0.37018831670117425 1.5165985679140255 8 0.2868517164804698 1.3430659332515902 9 0.28323323256382255 1.491889343733389 >10 1.4218293778290692 15.206598105412924 >50 0.09155468384370644 3.5081829263207727 >100 0.03647533954621153 3.667491790643112 >500 0.0025807483342365754 0.8326148483330607 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT 574 0.21618282890673254 No Hit CTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT 561 0.21128670211964626 No Hit CTTTATAAATTCATCTAATGGTCTAACTTTGGGAATTCATCTAATGGTCT 540 0.20337757423281458 No Hit CTTTATAAATTCATCTAATGGTCTAACTTTATAAATTCATCTAATGGTCT 530 0.19961132285813282 No Hit ATTCCCAAAGTTAGACCATTAGATGAATTTATAAAGTTAGACCATTAGAT 457 0.17211768782295606 No Hit GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC 388 0.14613055333765196 No Hit GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG 362 0.13633829976347941 No Hit GTTTCAATTGTGCACTGGTTTTCAAATTTTTTTAAACTGAGAATTATTGA 303 0.11411741665285709 No Hit CTATTTTTGGTGAACGGCCAGAGTCACTATTTTTGGTGAACGGCCAGAGT 295 0.11110441555311167 No Hit GTAATTTATTTGGTGAAGCCAGAGAGTAAAATAATTGGTGAAGCCAGAGA 286 0.1077147893158981 No Hit CCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTT 266 0.10018228656653459 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0011298754124045257 20-21 0.0 0.0 0.0028246885310113136 22-23 0.0 0.0 0.006402627336958977 24-25 0.0 0.0 0.010733816417842993 26-27 0.0 0.0 0.017136443754801972 28-29 0.0 0.0 0.03803913888428569 30-31 0.0 0.0 0.059883396857439854 32-33 0.0 0.0 0.08455234336160533 34-35 0.0 0.0 0.13276036095753174 36-37 0.0 0.0 0.2370855240362163 38-39 0.0 0.0 0.4624956688109191 40-41 0.0 0.0 0.8029647930821495 42-43 0.0 0.0 1.179966555687793 44-45 0.0 0.0 1.6420855993612438 46-47 0.0 0.0 2.178399795115925 48-49 0.0 0.0 2.753694692598563 50-51 0.0 0.0 3.446496632971271 52-53 0.0 0.0 4.113123126289941 54-55 0.0 0.0 4.791801624007593 56-57 0.0 0.0 5.557292215911659 58-59 0.0 0.0 6.341425752120399 60-61 0.0 0.0 7.2532352099308515 62-63 0.0 0.0 8.363526115187032 64-65 0.0 0.0 9.535395230419258 66-67 0.0 0.0 10.319152141490532 68-69 0.0 0.0 11.13360400126546 70-71 0.0 0.0 12.216024646348997 72-73 0.0 0.0 12.989236053571158 74-75 3.766251374681752E-4 0.0 13.74154476566384 76-77 3.766251374681752E-4 0.0 14.516827611142077 78-79 3.766251374681752E-4 0.0 15.237499811687432 80-81 3.766251374681752E-4 0.0 15.953464198014434 82-83 3.766251374681752E-4 0.0 16.660766206179666 84-85 3.766251374681752E-4 0.0 17.397444975067415 86-87 3.766251374681752E-4 0.0 18.239578782446255 88-89 3.766251374681752E-4 0.0 19.281700537820697 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACCA 35 2.784057E-4 54.285606 3 TGGTACC 35 2.784057E-4 54.285606 2 GGGTAAC 40 5.3757086E-4 47.50885 1 GCCCTAA 50 2.8179711E-5 47.50885 1 GATAGTC 70 7.89023E-8 47.499905 6 AGCGGCG 45 9.611778E-4 42.222137 7 CTCGGAG 45 9.611778E-4 42.222137 8 CTAACAT 125 1.3460522E-10 37.999924 2 ATACTTG 80 1.07450705E-5 35.624928 3 CCTAACA 140 4.5110937E-10 33.9349 1 TAACATA 160 5.2750693E-11 32.656185 3 GTGTAGG 75 3.0687643E-4 31.67257 1 CTATCCC 60 0.003945568 31.666603 4 AGCTTCC 60 0.003945568 31.666603 5 AAGCGAT 30 0.0039490936 31.666603 82-83 CATAGTC 90 2.4060631E-5 31.666603 3 TCATCGG 60 0.003945568 31.666603 4 TAACTCG 60 0.003945568 31.666603 5 CTTTATA 640 0.0 31.177683 1 CTTGGTG 110 2.7230635E-6 30.227213 6 >>END_MODULE