FastQCFastQC Report
Wed 17 Sep 2014
G50_Mov_M1_L36_GCTACGCT-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_Mov_M1_L36_GCTACGCT-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265516
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT5740.21618282890673254No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT5610.21128670211964626No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTGGGAATTCATCTAATGGTCT5400.20337757423281458No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTATAAATTCATCTAATGGTCT5300.19961132285813282No Hit
ATTCCCAAAGTTAGACCATTAGATGAATTTATAAAGTTAGACCATTAGAT4570.17211768782295606No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3880.14613055333765196No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3620.13633829976347941No Hit
GTTTCAATTGTGCACTGGTTTTCAAATTTTTTTAAACTGAGAATTATTGA3030.11411741665285709No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTATTTTTGGTGAACGGCCAGAGT2950.11110441555311167No Hit
GTAATTTATTTGGTGAAGCCAGAGAGTAAAATAATTGGTGAAGCCAGAGA2860.1077147893158981No Hit
CCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTT2660.10018228656653459No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCA352.784057E-454.2856063
TGGTACC352.784057E-454.2856062
GGGTAAC405.3757086E-447.508851
GCCCTAA502.8179711E-547.508851
GATAGTC707.89023E-847.4999056
AGCGGCG459.611778E-442.2221377
CTCGGAG459.611778E-442.2221378
CTAACAT1251.3460522E-1037.9999242
ATACTTG801.07450705E-535.6249283
CCTAACA1404.5110937E-1033.93491
TAACATA1605.2750693E-1132.6561853
GTGTAGG753.0687643E-431.672571
CTATCCC600.00394556831.6666034
AGCTTCC600.00394556831.6666035
AAGCGAT300.003949093631.66660382-83
CATAGTC902.4060631E-531.6666033
TCATCGG600.00394556831.6666034
TAACTCG600.00394556831.6666035
CTTTATA6400.031.1776831
CTTGGTG1102.7230635E-630.2272136
Produced by FastQC (version 0.11.2)