FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_M3_L47_AAGAGGCA-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_M3_L47_AAGAGGCA-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6122784
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT14900.020.3985964
GTATAAG14350.019.5241091
GTATATA27300.018.785911
TACACCG28950.017.7166585
TCTATAC15300.017.0717433
GTATTAT35850.016.954771
GTATTAG14700.016.4749431
TATACCG18450.016.4736635
TACACTG23300.016.3057465
GTATAGA17550.016.2347371
TATAGGG18150.015.9609892
CTAGGAG6050.015.6993333
TACACAG16650.015.6875485
GTATACT13650.015.6562044
GTTCTAG16800.015.2635521
TATACTG27900.015.1492945
GTGTATA19450.015.1371181
GTGCTAC20500.015.0567271
GTAGCAC20600.014.984863
TATACTT31100.014.9648465
Produced by FastQC (version 0.11.2)