FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_M3_L47_AAGAGGCA-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_M3_L47_AAGAGGCA-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6122784
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG22250.021.3480975
CTATACT14500.019.9825554
GTCCTAC7200.019.8069921
TATACAG22050.018.7413045
TATACCG20950.018.1382395
GTATAGA17050.017.8437181
CTACACC34100.017.829734
GTGTATA17750.017.4078351
GTGTAGA29150.016.959951
TACACCG31400.016.791235
TCTATAC17550.016.5110173
GCTACAC24800.016.4728163
CCTATAC16450.016.4600093
TTATACT28050.016.4258584
CTACACA19750.016.3542634
CCTACAC21800.016.3427773
TACACAG18000.016.0970575
TATACTG29000.016.0515615
GTATTAT35000.015.8908661
GTATAGG18650.015.8031121
Produced by FastQC (version 0.11.2)