FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_M1_L37_CGAGGCTG-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_M1_L37_CGAGGCTG-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930818
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT26020.2795390720849833No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT24730.2656802941069038No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTATAAATTCATCTAATGGTCT17100.18370938250012353No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTGGGAATTCATCTAATGGTCT17030.18295735578813474No Hit
ATTCCCAAAGTTAGACCATTAGATGAATTTATAAAGTTAGACCATTAGAT15350.1649087147004033No Hit
ATATAGTGGGAAGCCACAGAGAGTAAAATATAGTGGGAAGCCACAGAGAG13750.14771953271208765No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTATTTTTGGTGAACGGCCAGAGT11870.12752224387581676No Hit
CCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTT11670.1253735961272773No Hit
CTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT11200.12032427391820957No Hit
CCCACTATATTTTACTCTCTGTGGCTTCCCACTATATTTTACTCTCTGTG11110.11935738243136682No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC10730.11527495170914186No Hit
GTAAAATATAGTGGGAAGCCACAGAGAGTAAAATATAGTGGGAAGCCACA10380.1115148181491978No Hit
CCAAAAATAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT9630.10345738909217485No Hit
GTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTAGGTAGTCTA9310.10001955269451171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT3150.037.6839331
ATACTTG3050.037.3626943
GATAGTC2100.036.1765756
CTATGGC6150.033.9706883
CTTGGTG3300.033.0933466
GGTAGTA4350.032.746042
GGTACAT1900.032.487521
GGGTACA600.00395800531.6545051
CGGTAGT4750.030.9880941
GCGATAC804.503109E-429.6760981
TCTATGG7100.029.4253142
ATGGCTT7350.029.0704635
CCTCGAC1153.8751023E-628.9019372
CTATTAA9800.028.5859531
TATGGCT7400.028.2323974
ATCTGGT2550.027.9304475
CCCTTGA1405.823622E-727.132431
CTTTATA22600.027.1024191
CTGGGGT4050.026.9649496
ACTTGGT4300.026.5014465
Produced by FastQC (version 0.11.2)