FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_M1_L37_CGAGGCTG-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_M1_L37_CGAGGCTG-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930818
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAATTTAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT24660.26492826739491504No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT22860.24559043765805988No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTGGGAATTCATCTAATGGTCT17740.1905850552954498No Hit
CTTTATAAATTCATCTAATGGTCTAACTTTATAAATTCATCTAATGGTCT17370.1866100569606518No Hit
CCCACTATATTTTACTCTCTGTGGCTTCCCACTATATTTTACTCTCTGTG16420.1764039801550894No Hit
ATTCCCAAAGTTAGACCATTAGATGAATTTATAAAGTTAGACCATTAGAT15980.17167695510830258No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC12330.13246413369745752No Hit
CTAAATTTGGTGAACGGCCAGAGTCACTAAATTTGGTGAACGGCCAGAGT11630.1249438665775694No Hit
CTATTTTTGGTGAACGGCCAGAGTCACTATTTTTGGTGAACGGCCAGAGT11350.12193575972961417No Hit
CCAAATTTAGTGACTCTGGCCGTTCACCAAAAATAGTGACTCTGGCCGTT11140.11967967959364773No Hit
CCAAAAATAGTGACTCTGGCCGTTCACCAAATTTAGTGACTCTGGCCGTT10420.11194454769890569No Hit
ATATAGTGGGAAGCCACAGAGAGTAAAATATAGTGGGAAGCCACAGAGAG10040.10786211697668073No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG9470.10173847089334327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC3150.034.700981
CATTCGG3050.034.2805141
TCATGGG702.0531012E-433.928385
CTATGGC6000.033.249813
ATACCAT3600.032.9859244
CCCCCCA3200.032.6560679
AACCCCC3350.032.6117557
ACTTGGT3550.032.112495
GGGTCCC600.003940300631.683511
CGGTAGT3950.031.282451
CAAACCC2300.030.9780865
GCATCTA2800.030.5519561
TGGACTT1401.7053026E-830.5355425
TATGGCT6550.030.4578424
CTTGGTG3750.030.399836
AAACCCC3750.030.399836
ATGGCTT6650.029.999835
CAAGGGC1751.5097612E-1029.8569724
CTAAGCA3050.029.5899965
CTTTATA21400.029.3146461
Produced by FastQC (version 0.11.2)