FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_M1_L25_CAGAGAGG-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_M1_L25_CAGAGAGG-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32979
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC156.560526E-495.03
CTCCTAT200.002056187571.251
TCCAATG250.004978210757.0000042
TATAACA250.004978210757.0000042
AGTACTG250.004978210757.0000046
TAGTACT405.3118804E-447.5000045
TACATAA300.003924218531.66666830-31
AGAATCA350.00833529627.14285532-33
TTGTTTC650.00748965318.26923248-49
TCGGTGG650.00748965318.26923294-95
TCCTTGT2350.00161255599.09574494-95
Produced by FastQC (version 0.11.2)