FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_M1_L25_CAGAGAGG-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_M1_L25_CAGAGAGG-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32979
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCA250.004978210757.0000043
TAGGATA250.004978210757.0000045
TGCCGTC1050.007196544713.57142894-95
TATGCCG1504.0004373E-412.66666794-95
CGTATGC1900.00289134610.092-93
GGATCTC1900.00289134610.086-87
GAGGATC1950.00358521619.74358984-85
CTCGTAT1950.00358521619.74358990-91
GAGAGGA2350.00161255599.09574494-95
ATCTCGT2150.008011448.8372188-89
AGACCAG3600.0096932496.59722288-89
Produced by FastQC (version 0.11.2)