FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_D2_L34_CTCTCTAC-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_D2_L34_CTCTCTAC-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932966
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTGC6400.023.7470258
TACACTG8850.023.6098065
CCTAAGG1754.0605646E-621.7087543
TAGACAG6250.020.5147745
ACCGTGC2108.6242835E-720.3545918
ACTGTCC6550.020.30288
CTGTGCG3302.7284841E-1120.1495119
TACACCG4950.020.1463835
GTCGCCG12500.019.95679794-95
GGTCGCC15050.019.89049594-95
TCTATAC6250.019.7549673
TACACAG7050.019.534035
GTATACG4650.019.4021531
GCACCGT2953.0286174E-919.3176126
GTATAGG6300.018.8429431
GTACACT8700.018.0126534
CTATACT5850.017.8586984
GTATATA12050.017.7326971
TGGTCGC19450.017.71168794-95
GGACCTA2152.4005109E-517.6703766
Produced by FastQC (version 0.11.2)