FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_D2_L34_CTCTCTAC-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_D2_L34_CTCTCTAC-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932966
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5350.026.6586041
GCTGTCC5500.022.4542458
GTGTAGC5350.022.2155041
GCTACAC4650.021.4546573
CTACACT9300.021.4513284
TATACAG7850.021.1780625
GTATAGA6450.020.638031
GTGTAGG5750.019.8432731
GTAGCAC6950.019.8229583
TACACCG5150.019.3686735
ACCACGT7900.019.240258
AGGGGTG4250.018.9997465
ACCGTGC2502.174238E-718.9997468
GTATAGT7300.018.886221
CTGTCCC5300.018.8205039
CCTACAC7100.018.7350523
TAGCACT5400.018.4719754
GCACCGC3105.5897544E-918.3868526
TACACAG7600.018.1247585
CCGTTAC3156.7411747E-918.1109261
Produced by FastQC (version 0.11.2)