FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_D1_L35_GGACTCCT-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_D1_L35_GGACTCCT-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3861292
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG10150.020.5825271
TAGGGCG3900.019.4813045
CTAGTAC9200.018.5813523
TATACAG14500.018.3392965
CTATACT14250.018.3278064
ACTGTGC14300.017.6023248
ACTTACG28150.017.2061794
GGTCGCC29200.016.9227994-95
GTATAAG11850.016.8284051
ATACCGC6850.016.6373186
GTACAGG6600.016.546111
CTGTGCG6900.016.5188989
GTATACG10450.016.3567961
CCTAACA15150.016.2968141
ACCGTGC5850.016.2369448
ACTGTGT13800.016.1751738
GTCTTAC9150.016.0861681
GTACTAG8650.015.9181991
GCTACTT30750.015.9039181
TAGACCG5400.015.828565
Produced by FastQC (version 0.11.2)