FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_D1_L35_GGACTCCT-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_D1_L35_GGACTCCT-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3861292
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACA17250.020.6519994
TACACTG19000.019.7498325
TACACCG9750.019.4870155
GTCTTAG8600.018.7910771
GCTACAC9450.018.598933
CTACACT18100.018.370014
TATACAG16250.018.1229235
GGTAGTA14750.017.713322
CGGTAGT13350.017.4456041
TAGCCCG2755.915772E-717.2725815
TATACCG8850.017.1749975
GTGCTAG7500.017.1109331
CCTGCAC7500.017.1009623
GGACCGC3351.4602847E-817.0147826
CGTACAC7650.016.765653
CCTACAC15350.016.7110393
GTCCTAC5150.016.6125581
GTGTAGG13800.016.5323031
GTAGTAC21850.016.5226693
CTATACT14500.016.3791714
Produced by FastQC (version 0.11.2)