##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G50_MOV_D1_37_TCCTGAGC-AAGGAGTA_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101243 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37192694803591 33.0 33.0 33.0 33.0 33.0 2 32.38581432790415 33.0 33.0 33.0 33.0 33.0 3 32.37525557322481 33.0 33.0 33.0 33.0 33.0 4 36.22765030668787 37.0 37.0 37.0 37.0 37.0 5 36.2017818515848 37.0 37.0 37.0 37.0 37.0 6 36.24281184872041 37.0 37.0 37.0 37.0 37.0 7 36.20791560898037 37.0 37.0 37.0 37.0 37.0 8 36.22133875922286 37.0 37.0 37.0 37.0 37.0 9 36.22165483045742 37.0 37.0 37.0 37.0 37.0 10-11 36.18963286350662 37.0 37.0 37.0 37.0 37.0 12-13 36.16461878845945 37.0 37.0 37.0 37.0 37.0 14-15 38.387750264215796 40.0 37.0 40.0 37.0 40.0 16-17 38.33585531839238 40.0 37.0 40.0 37.0 40.0 18-19 38.26711970210286 40.0 37.0 40.0 37.0 40.0 20-21 38.21447408709738 40.0 37.0 40.0 37.0 40.0 22-23 38.132749918512886 40.0 37.0 40.0 37.0 40.0 24-25 38.01970506602926 40.0 37.0 40.0 37.0 40.0 26-27 37.86432642256749 40.0 37.0 40.0 35.0 40.0 28-29 37.73712256649843 40.0 37.0 40.0 33.0 40.0 30-31 37.62547040289205 40.0 37.0 40.0 33.0 40.0 32-33 37.46747429451913 40.0 37.0 40.0 33.0 40.0 34-35 37.46142943215827 40.0 37.0 40.0 33.0 40.0 36-37 37.61098545084599 40.0 37.0 40.0 33.0 40.0 38-39 37.56449334768824 40.0 37.0 40.0 33.0 40.0 40-41 37.406497239315314 40.0 37.0 40.0 33.0 40.0 42-43 37.2591932281738 40.0 37.0 40.0 33.0 40.0 44-45 37.06980235670614 40.0 37.0 40.0 33.0 40.0 46-47 36.92354039291605 40.0 37.0 40.0 33.0 40.0 48-49 36.73101844078109 38.5 37.0 40.0 33.0 40.0 50-51 35.67117232796342 37.0 35.0 38.5 30.0 40.0 52-53 35.849199450826234 37.0 37.0 38.5 33.0 40.0 54-55 36.594490483292674 37.0 37.0 40.0 33.0 40.0 56-57 36.54845767114764 37.0 37.0 40.0 33.0 40.0 58-59 36.3510514307162 37.0 37.0 40.0 33.0 40.0 60-61 36.14800035558014 37.0 37.0 40.0 33.0 40.0 62-63 35.926380095414004 37.0 37.0 40.0 33.0 40.0 64-65 35.702428809893036 37.0 37.0 40.0 33.0 40.0 66-67 35.43121499758008 37.0 37.0 37.0 33.0 40.0 68-69 35.21718044704325 37.0 35.0 37.0 33.0 40.0 70-71 34.984201376885316 37.0 33.0 37.0 30.0 40.0 72-73 34.74070306095236 37.0 33.0 37.0 27.0 40.0 74-75 34.52831800717087 37.0 33.0 37.0 27.0 37.0 76-77 34.295566113212764 37.0 33.0 37.0 27.0 37.0 78-79 34.071861758343786 37.0 33.0 37.0 27.0 37.0 80-81 33.8901998162836 37.0 33.0 37.0 27.0 37.0 82-83 33.66484596465928 37.0 33.0 37.0 27.0 37.0 84-85 33.459458925555346 37.0 33.0 37.0 27.0 37.0 86-87 33.276675918335094 37.0 33.0 37.0 27.0 37.0 88-89 33.07786711180032 37.0 33.0 37.0 27.0 37.0 90-91 32.861822545756254 37.0 33.0 37.0 24.5 37.0 92-93 32.648084312001814 37.0 33.0 37.0 22.0 37.0 94-95 32.42125875369162 37.0 33.0 37.0 22.0 37.0 96-97 32.17680234682892 35.0 33.0 37.0 22.0 37.0 98-99 31.884159892535777 33.0 33.0 37.0 22.0 37.0 100-101 30.865146232332112 33.0 30.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.020317088825926533 2306 2 0.2889459913648196 2306 3 0.31250186537246094 2306 4 0.5341690063288524 2306 5 0.23547091297687217 2306 6 0.4410245418655876 2306 7 0.280938687060015 2306 8 0.4620635241295119 2306 9 0.6013685270143156 2306 10-11 0.6129637178259983 2306 12-13 0.6836458183754601 2306 14-15 0.4036284191347832 2306 16-17 0.5473721242265412 2306 18-19 0.6953999867983924 2306 20-21 0.5132613917938116 2306 22-23 0.7501748610077072 2306 24-25 1.0271820631900326 2306 26-27 0.7417917553412465 2306 28-29 0.554066346465909 2306 30-31 0.7565706452909993 2306 32-33 0.062198732114815414 2306 34-35 0.5192691987247073 2306 36-37 0.4912227478219151 2306 38-39 0.7208844710819662 2306 40-41 0.10449109226522069 2306 42-43 0.49802930834390935 2306 44-45 0.34452590955189777 2306 46-47 0.49845388942915747 2306 48-49 0.8176353628079909 2306 50-51 0.23032182018658887 2306 52-53 0.41732156864261327 2306 54-55 0.7262569651346737 2306 56-57 0.818341467260133 2306 58-59 0.9703019954573762 2306 60-61 0.6653545609938618 2306 62-63 0.4340922388236095 2306 64-65 1.109679945881112 2306 66-67 0.7277211328572974 2306 68-69 0.5836987901675172 2306 70-71 0.9545387142386588 2306 72-73 1.2235715276738617 2306 74-75 0.6084454628995388 2306 76-77 0.9461268293544975 2306 78-79 0.6395207449872018 2306 80-81 0.42759609108571084 2306 82-83 0.8408453362169794 2306 84-85 0.5796849494806722 2306 86-87 0.4972589805317966 2306 88-89 0.32398701116621 2306 90-91 0.1113288135767192 2306 92-93 0.3424820117466112 2306 94-95 -0.2087788347737103 2306 96-97 -0.5345603341000924 2306 98-99 -0.06706074661558148 2306 100-101 0.10461268636632681 2307 1 0.1761301421040642 2307 2 0.019353514875788846 2307 3 0.10351544948949254 2307 4 -0.2920239577951449 2307 5 -0.1678728696772538 2307 6 -0.1922042982598029 2307 7 0.2732758518109719 2307 8 0.051056906226342846 2307 9 0.18827204495231342 2307 10-11 0.2458442472582547 2307 12-13 0.3541439026666495 2307 14-15 0.33135395030859627 2307 16-17 0.6704999542509213 2307 18-19 0.6644003351090859 2307 20-21 0.07142927754790662 2307 22-23 0.3125224750794615 2307 24-25 0.2777916069030226 2307 26-27 0.22141557979265514 2307 28-29 0.28917606433424936 2307 30-31 0.09460617298169893 2307 32-33 0.3849085893074218 2307 34-35 0.1585934759800125 2307 36-37 -0.04360832149190941 2307 38-39 0.1595817890896285 2307 40-41 0.46673421312902974 2307 42-43 0.5375625720150978 2307 44-45 0.4951702843342076 2307 46-47 0.7551588702720693 2307 48-49 0.38503348193025033 2307 50-51 0.08437963976165008 2307 52-53 0.190745462756162 2307 54-55 0.2950483270294839 2307 56-57 0.3822564794510086 2307 58-59 0.27629293937935984 2307 60-61 0.19565759129688587 2307 62-63 0.12183170242514052 2307 64-65 0.34914354741367504 2307 66-67 -0.063814917299446 2307 68-69 -0.5845352746182684 2307 70-71 0.14767699358383624 2307 72-73 -0.3687308060077825 2307 74-75 -0.3713525168984404 2307 76-77 -0.1261476394716965 2307 78-79 -0.3113674472803041 2307 80-81 -0.6072349782281208 2307 82-83 -0.3777196237272946 2307 84-85 -1.7238678195893442 2307 86-87 -0.7223509114918869 2307 88-89 -1.1549401918989268 2307 90-91 -1.0934778739885829 2307 92-93 -1.2729829830287223 2307 94-95 -1.5504716247423609 2307 96-97 -1.6679633295720535 2307 98-99 -2.27604716249855 2307 100-101 -1.8016917371794712 2201 1 0.24322970920363218 2201 2 0.31991010114666096 2201 3 0.221016995562465 2201 4 0.05481174618341811 2201 5 0.12897314145446614 2201 6 0.1658662026678357 2201 7 0.1258835615615368 2201 8 0.4522113073807432 2201 9 0.5096490806150626 2201 10-11 0.40395597679855655 2201 12-13 0.5638944695600685 2201 14-15 0.502091942475154 2201 16-17 0.5742311436964016 2201 18-19 0.6182242889330425 2201 20-21 0.05437093906099477 2201 22-23 0.3733862145146247 2201 24-25 0.5615475335160909 2201 26-27 0.8092325247524528 2201 28-29 0.4580072331654108 2201 30-31 0.7094793949977785 2201 32-33 0.8886707002123941 2201 34-35 0.9969257785980332 2201 36-37 0.32461472530255975 2201 38-39 0.41751829702614174 2201 40-41 0.5419764312283917 2201 42-43 0.26550526424350807 2201 44-45 0.2820698926623848 2201 46-47 0.06865093376413967 2201 48-49 0.6772412741380407 2201 50-51 0.10793148474206049 2201 52-53 0.04891903521544094 2201 54-55 0.44259522457638667 2201 56-57 0.7903682089913033 2201 58-59 0.2625844256708376 2201 60-61 -0.030223670298660466 2201 62-63 0.4387251264614207 2201 64-65 0.24256758369485 2201 66-67 0.42458953539072297 2201 68-69 -0.12396013547170526 2201 70-71 -0.279451433544601 2201 72-73 -0.26241252111806546 2201 74-75 -0.5174048772365154 2201 76-77 -0.4503076064888063 2201 78-79 -0.5233340521040546 2201 80-81 -1.630168394018682 2201 82-83 -1.3747305493096391 2201 84-85 -1.4203736946666439 2201 86-87 -0.8799988191398 2201 88-89 -0.08974744813475866 2201 90-91 -0.477986222782647 2201 92-93 -0.5344239230410892 2201 94-95 -0.9086967329674707 2201 96-97 -1.0705091964036342 2201 98-99 -1.6109265688065264 2201 100-101 -1.7295416650294015 1101 1 0.4185543845283064 1101 2 0.44359593911821094 1101 3 0.4065457525197971 1101 4 0.07161240584122197 1101 5 0.45838975658536896 1101 6 0.35325024719473674 1101 7 0.41468999322511024 1101 8 0.3540872092566474 1101 9 0.4711003277805972 1101 10-11 0.38725838867239304 1101 12-13 0.3894974380201859 1101 14-15 0.6091317280863748 1101 16-17 0.029085812836775915 1101 18-19 0.48258215329090604 1101 20-21 0.5209242270428547 1101 22-23 0.6256537882107764 1101 24-25 0.5707208998323168 1101 26-27 0.5800014383785097 1101 28-29 0.7740245491827338 1101 30-31 0.2865253649008892 1101 32-33 0.32430252870135945 1101 34-35 0.047482364868905336 1101 36-37 0.4513411734575854 1101 38-39 0.8717030012108395 1101 40-41 0.4970372434320609 1101 42-43 -0.9270838926313658 1101 44-45 -0.004829715665792378 1101 46-47 0.027886142999342667 1101 48-49 0.5921041890009562 1101 50-51 -0.20854965316764407 1101 52-53 0.22104849305919316 1101 54-55 0.6738362058173664 1101 56-57 0.5842766814712093 1101 58-59 0.2914444545308754 1101 60-61 0.4936373892766852 1101 62-63 0.79354887414231 1101 64-65 0.6824768807470036 1101 66-67 0.6382052847207618 1101 68-69 0.47607078598779395 1101 70-71 0.48101032691716483 1101 72-73 0.6767237394467642 1101 74-75 0.7195565740106531 1101 76-77 0.808195794871736 1101 78-79 0.6987335628207063 1101 80-81 0.9382195672264189 1101 82-83 0.4657147197070586 1101 84-85 1.0135059177843928 1101 86-87 1.3332046440636702 1101 88-89 1.2592012222424884 1101 90-91 0.7701584896477769 1101 92-93 0.7926735826278417 1101 94-95 0.853568779298044 1101 96-97 0.872440710831988 1101 98-99 1.138094251642869 1101 100-101 0.809419373931636 2304 1 -0.5150120490381305 2304 2 -0.7332688627465878 2304 3 -0.5943283733543296 2304 4 -0.6569229476941345 2304 5 -0.354935806740194 2304 6 -0.4754210814765898 2304 7 -0.8207412422061253 2304 8 -0.3582282530588188 2304 9 -0.4701389884587215 2304 10-11 -0.7731437092297 2304 12-13 -0.7639116528889076 2304 14-15 -0.36731543586078885 2304 16-17 -0.4480412267902594 2304 18-19 -0.4209240627153079 2304 20-21 -0.4972381661195371 2304 22-23 -0.7290470664900823 2304 24-25 -0.304327662716247 2304 26-27 -0.23079887242179353 2304 28-29 -0.4437784811202974 2304 30-31 -0.9754695845940589 2304 32-33 -0.9164705870717498 2304 34-35 -1.1854721805856414 2304 36-37 -0.5669669073504977 2304 38-39 -0.8826190656112303 2304 40-41 -0.7050800836852602 2304 42-43 -0.8296674202148893 2304 44-45 -0.43349133182741184 2304 46-47 -0.34687104425784554 2304 48-49 -0.3138301544333899 2304 50-51 -0.32466270678069975 2304 52-53 -0.7660338690231754 2304 54-55 -0.6549322479510877 2304 56-57 -0.7136545539600192 2304 58-59 -1.0437342556478413 2304 60-61 -0.4227864646471673 2304 62-63 -0.19333923774580342 2304 64-65 0.1493572226273443 2304 66-67 -0.38418205016657936 2304 68-69 -0.4228219879049888 2304 70-71 -0.0898909939841559 2304 72-73 -0.4898458021227796 2304 74-75 -0.2841827422286656 2304 76-77 -0.5219324102564613 2304 78-79 -1.0722940207068774 2304 80-81 -0.3934140769072201 2304 82-83 0.11300492949726504 2304 84-85 -0.23071513893666662 2304 86-87 -0.39304826968924544 2304 88-89 -0.4092136961724293 2304 90-91 -0.2962750767857898 2304 92-93 -0.6325400925858276 2304 94-95 -0.17037255714329547 2304 96-97 -0.5405837646924923 2304 98-99 -0.5444038058551897 2304 100-101 -0.7473988078865439 2202 1 0.4128725663464863 2202 2 0.0856413936636713 2202 3 0.27586393433797696 2202 4 0.2180770523058655 2202 5 0.3245513727469884 2202 6 0.1543866108311036 2202 7 0.12397029625541478 2202 8 0.5712589264283636 2202 9 -0.015010783330517086 2202 10-11 0.4279780856420885 2202 12-13 0.5277550137777638 2202 14-15 0.44798905131869304 2202 16-17 0.2610933885943538 2202 18-19 0.3146528603616119 2202 20-21 0.10772978259840471 2202 22-23 0.3322510104329979 2202 24-25 0.4697107988222129 2202 26-27 0.6101466403987104 2202 28-29 0.9520548522130312 2202 30-31 0.5505720820726125 2202 32-33 0.7662217206205568 2202 34-35 0.2520278194143586 2202 36-37 0.656991425982838 2202 38-39 0.7620123446451785 2202 40-41 0.650288506058331 2202 42-43 0.42834540029792834 2202 44-45 0.9154670015059168 2202 46-47 0.6371033147165193 2202 48-49 0.8736698455666101 2202 50-51 0.8387988316808404 2202 52-53 0.7668186950793938 2202 54-55 0.9760773674335255 2202 56-57 1.069598651168178 2202 58-59 1.0676439494803702 2202 60-61 0.9153545609938547 2202 62-63 0.8319011468695834 2202 64-65 1.2519213251914465 2202 66-67 1.3485903857308656 2202 68-69 0.9353510890180914 2202 70-71 1.1208461855030194 2202 72-73 0.738118941466972 2202 74-75 1.327195462899546 2202 76-77 1.0217374615384003 2202 78-79 1.200943158780298 2202 80-81 1.2391981025799552 2202 82-83 0.8866427500100826 2202 84-85 0.4712079379864136 2202 86-87 0.5031857046697326 2202 88-89 0.8363792525455196 2202 90-91 0.8591736411629327 2202 92-93 0.44718747151672744 2202 94-95 0.6545473146515732 2202 96-97 0.7398649532562231 2202 98-99 -0.004740344316729761 2202 100-101 -0.4661488078865439 1102 1 0.10037256634648628 1102 2 0.11480806033033275 1102 3 0.13836393433797411 1102 4 -0.04192294769413252 1102 5 -0.15878196058634586 1102 6 -0.1231133891688998 1102 7 0.05772029625541819 1102 8 0.07792559309503133 1102 9 0.03332255000282203 1102 10-11 -0.07910524769123839 1102 12-13 0.1385883471110958 1102 14-15 0.13403071798536104 1102 16-17 0.3348433885943578 1102 18-19 0.12465286036161416 1102 20-21 0.19314644926507896 1102 22-23 0.09787601043299787 1102 24-25 0.1397107988222146 1102 26-27 -0.13464502626795394 1102 28-29 0.05872151887970034 1102 30-31 0.17703041540594455 1102 32-33 0.026221720620554834 1102 34-35 -0.057972180585643684 1102 36-37 -0.07446690735049799 1102 38-39 0.25680401131184993 1102 40-41 0.40299683939166187 1102 42-43 0.49417873363126574 1102 44-45 1.0140086681725933 1102 46-47 0.5229366480498427 1102 48-49 0.5186698455666061 1102 50-51 0.5462988316808364 1102 52-53 0.6447353617460578 1102 54-55 0.38045236743352717 1102 56-57 0.5473069845015104 1102 58-59 0.5547272828137011 1102 60-61 0.6670212276605199 1102 62-63 0.49127614686958054 1102 64-65 0.5035879918581116 1102 66-67 0.5875487190641948 1102 68-69 0.2991010890180874 1102 70-71 0.38626285216969336 1102 72-73 0.45707727480030513 1102 74-75 0.061778796232879074 1102 76-77 -0.04347087179493059 1102 78-79 -0.008640174553029567 1102 80-81 0.2702397692466221 1102 82-83 0.7293510833434169 1102 84-85 0.008707937986407899 1102 86-87 -0.08785596199693657 1102 88-89 -0.5538290807878141 1102 90-91 0.14334030782960383 1102 92-93 -0.28677086181659917 1102 94-95 -0.3744110186817551 1102 96-97 -0.1684683800771083 1102 98-99 0.10713465568326797 1102 100-101 0.10260119211345753 2305 1 0.2474313898758993 2305 2 0.38833747209503855 2305 3 0.4118933461026799 2305 4 0.6004299934823365 2305 5 0.3098454903940464 2305 6 0.5208081794585553 2305 7 0.021641864882866457 2305 8 0.11047461270287329 2305 9 0.43842058921850224 2305 10-11 0.11363985034797253 2305 12-13 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-1.244680865674006 1213 82-83 -0.9722362182438857 1213 84-85 -0.9424825382040609 1213 86-87 -1.506982946123923 1213 88-89 -2.3573211442798794 1213 90-91 -1.8521358826465928 1213 92-93 -0.7073264173721583 1213 94-95 -0.20631578058651456 1213 96-97 -0.6271985388072636 1213 98-99 -0.7038970903484767 1213 100-101 -1.1462083316960694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 4.0 6 4.0 7 38.0 8 131.0 9 148.0 10 211.0 11 243.0 12 198.0 13 203.0 14 204.0 15 185.0 16 218.0 17 199.0 18 232.0 19 220.0 20 239.0 21 272.0 22 284.0 23 298.0 24 367.0 25 403.0 26 476.0 27 614.0 28 770.0 29 993.0 30 1225.0 31 1613.0 32 2267.0 33 3356.0 34 5200.0 35 9066.0 36 17777.0 37 36741.0 38 16826.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.994587226645066 21.321190810138084 10.470950791173623 24.213271172043225 2 16.50385705678417 20.94959651531464 38.63081892081428 23.91572750708691 3 19.875942040437362 25.687701865808005 30.540383038827372 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27.894099923439775 22.320021733224667 24-25 22.362954656602433 28.09423364767602 27.517746612459433 22.025065083262117 26-27 22.091317298715154 28.2853430943948 27.53745609745254 22.085883509437505 28-29 22.116135023264544 28.10761950862912 27.545516512392936 22.230728955713396 30-31 22.009661549404303 28.04417837314524 27.76756959674392 22.178590480706536 32-33 22.160092864728693 28.476870261058558 27.242954876633156 22.12008199757959 34-35 21.800172903544524 27.755464987032237 28.070643448190687 22.373718661232555 36-37 22.079115471832342 28.069343372214234 27.823805783225385 22.027735372728035 38-39 22.094218076907875 27.916156187013396 27.66865589060586 22.320969845472867 40-41 22.166725950517748 27.792269385819303 27.78387084024978 22.25713382341317 42-43 22.325089665938798 27.576598919068463 27.801381300082006 22.29693011491073 44-45 21.81888242820656 27.51591979093078 28.062799808320282 22.602397972542374 46-47 21.66764649214246 27.431984467707753 28.378051250636062 22.52231778951373 48-49 21.53951801752843 27.35087493948047 28.348335589435514 22.761271453555583 50-51 21.499925892989477 27.759992095252212 28.192282989970852 22.547799021787462 52-53 21.25965191358604 27.640412803019448 28.57411039368445 22.525824889710062 54-55 21.263110052810728 27.6083015102188 28.47629445561478 22.65229398135569 56-57 21.16568357194376 27.54344883461284 28.503818754878424 22.787048838564978 58-59 20.878187108790268 27.582169481812297 28.718931710281254 22.82071169911618 60-61 21.125445301421504 27.625734345894827 28.39899007366928 22.849830279014384 62-63 21.257158103294085 26.965853562129126 28.556817676500668 23.22017065807612 64-65 21.10895374171842 27.085031644162505 28.454055442746544 23.35195917137253 66-67 21.499048889547666 27.021418513303196 27.98092838261815 23.498604214530992 68-69 21.396210567920754 26.980064721721302 27.97608754724439 23.64763716311356 70-71 21.457791919052184 27.012084860821535 27.972549678362864 23.55757354176342 72-73 21.067793260203576 27.197845636920643 27.998814112066412 23.735546990809368 74-75 21.342596252668194 26.973476164123646 27.58666693019211 24.097260653016047 76-77 21.135989642272968 27.0022385736382 27.893714697990223 23.968057086098607 78-79 21.054555875058053 26.995326040771157 28.026462712082136 23.923655372088657 80-81 21.14778398142201 26.680666040812294 28.022629576560103 24.148920401205594 82-83 21.024237453931054 26.40726431966168 28.531623306688275 24.036874919718993 84-85 21.04241286465924 26.580045945211573 28.48701924264506 23.890521947484128 86-87 21.361660079051383 26.616600790513832 28.274209486166008 23.747529644268774 88-89 21.769332621884942 26.10521531195036 28.40605916879113 23.71939289737357 90-91 21.6923897792621 26.202497875620022 28.443471730925047 23.661640614192834 92-93 21.648424953675107 25.97504632489191 28.464484249536753 23.91204447189623 94-95 21.635898246793033 25.470420809202853 28.664538572529796 24.229142371474317 96-97 22.343464294539164 25.561650605386703 28.1527057079318 23.942179392142325 98-99 22.604222261756508 25.341971574848287 28.299630354424878 23.75417580897033 100-101 22.678323440889976 25.381159713951064 28.163069639177042 23.77744720598192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 1.0 3 2.0 4 1.5 5 3.0 6 6.0 7 5.5 8 4.0 9 6.0 10 8.0 11 10.5 12 14.0 13 12.5 14 15.5 15 20.5 16 20.5 17 36.5 18 57.5 19 83.0 20 107.5 21 147.0 22 225.0 23 307.5 24 422.5 25 578.0 26 762.5 27 934.0 28 1080.0 29 1244.5 30 1392.0 31 1630.0 32 1899.5 33 2012.5 34 2132.0 35 2331.5 36 2544.5 37 2702.5 38 2816.0 39 3025.5 40 3208.5 41 3267.0 42 3336.5 43 3477.0 44 3623.0 45 3704.5 46 3743.5 47 3768.0 48 3807.0 49 3887.0 50 3908.5 51 3859.0 52 3741.5 53 3569.5 54 3262.5 55 2901.0 56 2598.0 57 2232.5 58 1862.5 59 1564.5 60 1342.5 61 1104.5 62 891.0 63 730.5 64 593.5 65 503.0 66 451.0 67 378.0 68 279.0 69 222.0 70 185.0 71 160.5 72 136.5 73 104.5 74 71.5 75 46.5 76 32.0 77 21.5 78 17.5 79 13.5 80 9.5 81 5.0 82 2.5 83 1.5 84 1.5 85 3.0 86 2.5 87 0.0 88 2.5 89 3.0 90 1.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.87722607982774E-4 2 0.0 3 0.0 4 0.0 5 0.001975445215965548 6 0.015803561727724385 7 0.03555801388737987 8 0.041484349535276514 9 0.03555801388737987 10-11 0.03259484606343155 12-13 0.03753345910334542 14-15 0.022223758679612418 16-17 0.007901780863862192 18-19 0.010371087383819129 20-21 0.017285145639698546 22-23 0.015309700423732999 24-25 0.026174649111543515 26-27 0.024199203895577965 28-29 0.015803561727724385 30-31 0.01679128433570716 32-33 0.020248313463646868 34-35 0.03012553954347461 36-37 0.036051875191371256 38-39 0.030619400847465998 40-41 0.03457029127939709 42-43 0.03358256867141432 44-45 0.03210098475944016 46-47 0.034076429975405706 48-49 0.03555801388737987 50-51 0.03753345910334542 52-53 0.03210098475944016 54-55 0.03210098475944016 56-57 0.03160712345544877 58-59 0.034076429975405706 60-61 0.04691682387918177 62-63 0.045929101271198994 64-65 0.03901504301531958 66-67 0.04494137866321622 68-69 0.04000276562330235 70-71 0.041484349535276514 72-73 0.05234929822308703 74-75 0.05037385300712148 76-77 0.06173266299892338 78-79 0.043459794751242065 80-81 0.04741068518317316 82-83 0.03555801388737987 84-85 0.03506415258338848 86-87 0.042472072143259286 88-89 0.040496626927293736 90-91 0.03654573649536264 92-93 0.05481860474304397 94-95 0.05630018865501813 96-97 0.06716513734282864 98-99 0.06420196951888033 100-101 0.07062216647076834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 101243.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.60976667050632 #Duplication Level Percentage of deduplicated Percentage of total 1 98.24856611557472 95.9001961425306 2 1.4881678462511836 2.905194324782559 3 0.13367852637177946 0.39144989304019556 4 0.06832545882298355 0.26676928373466846 5 0.011962903145183688 0.058384809235162295 6 0.02071559196849226 0.12132264590915665 7 0.009219085558715527 0.06299109532111599 8 0.010464766342857701 0.08171707207901663 9 0.0 0.0 >10 0.007872308818083831 0.15982705560242733 >50 0.001027397146031489 0.05214767776508042 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 0.0 16-17 0.0 0.0 0.0 18-19 0.0 0.0 0.0 20-21 0.0 0.0 0.0 22-23 0.0 0.0 9.87722607982774E-4 24-25 0.0 0.0 0.001481583911974161 26-27 0.0 0.0 0.005432474343905257 28-29 0.0 4.93861303991387E-4 0.02370534259158658 30-31 0.0 9.87722607982774E-4 0.044447517359224836 32-33 0.0 0.001975445215965548 0.06321424691089754 34-35 0.0 0.001975445215965548 0.10716790296613099 36-37 0.0 0.001975445215965548 0.28100708197109925 38-39 0.0 0.001975445215965548 0.5985599004375611 40-41 0.0 0.001975445215965548 1.0677281392293787 42-43 0.0 0.001975445215965548 1.591221121460249 44-45 0.0 0.001975445215965548 2.3018875379038555 46-47 0.0 0.001975445215965548 3.1276236381774543 48-49 0.0 0.001975445215965548 3.8896516302361643 50-51 0.0 0.001975445215965548 4.578094288000158 52-53 0.0 0.001975445215965548 5.302094959651532 54-55 0.0 0.001975445215965548 6.1169661112373195 56-57 0.0 0.001975445215965548 6.99999012277392 58-59 0.0 0.001975445215965548 7.906225615598116 60-61 0.0 0.001975445215965548 8.739863496735577 62-63 0.0 0.001975445215965548 9.533992473553727 64-65 0.0 0.001975445215965548 10.377013719467024 66-67 0.0 0.001975445215965548 11.305472970970833 68-69 0.0 0.001975445215965548 12.197880347283268 70-71 0.0 0.001975445215965548 13.057692877532274 72-73 0.0 0.001975445215965548 13.858242051302312 74-75 0.0 0.001975445215965548 14.659778947680334 76-77 0.0 0.001975445215965548 15.488478215777882 78-79 0.0 0.001975445215965548 16.313226593443495 80-81 0.0 0.001975445215965548 17.12266527068538 82-83 0.0 0.001975445215965548 17.932597809231254 84-85 0.0 0.001975445215965548 18.7212943117055 86-87 0.0 0.001975445215965548 19.513447843307688 88-89 0.0 0.001975445215965548 20.296218010134034 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTGG 50 0.0016069664 38.009094 6 TCCACAG 50 0.0016069664 38.009094 5 TATCCTG 50 0.0016069664 38.009094 5 GTATTGG 55 0.0025655518 34.553722 1 GTATTAG 55 0.0025655518 34.553722 1 TACACTA 55 0.0025655518 34.553722 5 CGTGCGA 30 0.00393871 31.674248 10-11 GCAGGAT 80 4.4587898E-4 29.694605 3 GTATAAA 70 0.008346323 27.149353 1 CTAAGCC 100 0.0016409894 23.755684 3 TTGCACA 100 0.0016409894 23.755684 4 GCCTAAG 105 0.0021794678 22.624462 1 CGCCGTA 80 4.9541217E-5 20.786224 94-95 TCGCCGT 70 4.8249634E-4 20.362015 94-95 TAAGCCT 125 0.0059857457 19.004547 4 GTCGCCG 140 3.4366167E-8 18.66518 92-93 TAGTCTA 135 0.009330117 17.596804 9 GTAGTCT 185 0.0031119504 15.409093 8 GGTGGTC 180 6.009177E-7 14.517363 94-95 TGGTCGC 165 3.900179E-6 14.397385 90-91 >>END_MODULE