FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_D1_37_TCCTGAGC-AAGGAGTA_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_D1_37_TCCTGAGC-AAGGAGTA_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101243
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGG500.001606966438.0090946
TCCACAG500.001606966438.0090945
TATCCTG500.001606966438.0090945
GTATTGG550.002565551834.5537221
GTATTAG550.002565551834.5537221
TACACTA550.002565551834.5537225
CGTGCGA300.0039387131.67424810-11
GCAGGAT804.4587898E-429.6946053
GTATAAA700.00834632327.1493531
CTAAGCC1000.001640989423.7556843
TTGCACA1000.001640989423.7556844
GCCTAAG1050.002179467822.6244621
CGCCGTA804.9541217E-520.78622494-95
TCGCCGT704.8249634E-420.36201594-95
TAAGCCT1250.005985745719.0045474
GTCGCCG1403.4366167E-818.6651892-93
TAGTCTA1350.00933011717.5968049
GTAGTCT1850.003111950415.4090938
GGTGGTC1806.009177E-714.51736394-95
TGGTCGC1653.900179E-614.39738590-91
Produced by FastQC (version 0.11.2)