##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename G50_MOV_D1_37_TCCTGAGC-AAGGAGTA_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101243 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.568444238120165 33.0 33.0 33.0 33.0 33.0 2 32.60028841500153 33.0 33.0 33.0 33.0 33.0 3 32.59818456584653 33.0 33.0 33.0 33.0 33.0 4 36.48994992246377 37.0 37.0 37.0 37.0 37.0 5 36.3281609592762 37.0 37.0 37.0 37.0 37.0 6 36.36939837815948 37.0 37.0 37.0 37.0 37.0 7 36.313443892417254 37.0 37.0 37.0 37.0 37.0 8 36.31042146123683 37.0 37.0 37.0 37.0 37.0 9 36.33863081892081 37.0 37.0 37.0 37.0 37.0 10-11 36.28547158815918 37.0 37.0 37.0 35.0 37.0 12-13 36.25629426231937 37.0 37.0 37.0 33.0 37.0 14-15 38.11321770394002 40.0 37.0 40.0 37.0 40.0 16-17 38.04159299902216 40.0 37.0 40.0 35.0 40.0 18-19 37.941892278972375 40.0 37.0 40.0 33.0 40.0 20-21 37.905193445472776 40.0 37.0 40.0 33.0 40.0 22-23 37.84304593897849 40.0 37.0 40.0 33.0 40.0 24-25 37.74427861679326 40.0 37.0 40.0 33.0 40.0 26-27 37.61329178313562 40.0 37.0 40.0 33.0 40.0 28-29 37.50281007081971 40.0 37.0 40.0 33.0 40.0 30-31 37.37278132809182 40.0 37.0 40.0 33.0 40.0 32-33 37.22223264818308 40.0 37.0 40.0 33.0 40.0 34-35 37.08873206048813 37.0 37.0 40.0 33.0 40.0 36-37 36.97640824550833 37.0 37.0 40.0 33.0 40.0 38-39 36.78680007506692 37.0 37.0 40.0 33.0 40.0 40-41 36.59368055075413 37.0 37.0 40.0 33.0 40.0 42-43 36.40296613099177 37.0 37.0 40.0 33.0 40.0 44-45 36.169349979751686 37.0 37.0 40.0 33.0 40.0 46-47 35.9641802396215 37.0 37.0 40.0 33.0 40.0 48-49 35.957152593265704 37.0 35.0 40.0 30.0 40.0 50-51 36.23330995723161 37.0 37.0 40.0 33.0 40.0 52-53 36.148197900101735 37.0 37.0 40.0 33.0 40.0 54-55 35.93775865985796 37.0 37.0 40.0 33.0 40.0 56-57 35.71342710113292 37.0 33.0 40.0 33.0 40.0 58-59 35.471123929555624 37.0 33.0 40.0 27.0 40.0 60-61 35.23560641229517 37.0 33.0 38.5 27.0 40.0 62-63 34.97064982270379 37.0 33.0 37.0 27.0 40.0 64-65 34.701327499185126 37.0 33.0 37.0 27.0 40.0 66-67 34.4506928874095 37.0 33.0 37.0 27.0 40.0 68-69 34.1902254970714 37.0 33.0 37.0 27.0 40.0 70-71 33.934568315834184 37.0 33.0 37.0 27.0 38.5 72-73 33.672051401084516 37.0 33.0 37.0 27.0 37.0 74-75 33.409435714074064 37.0 33.0 37.0 27.0 37.0 76-77 32.47487233685292 33.0 33.0 37.0 27.0 37.0 78-79 33.59455962387523 37.0 33.0 37.0 27.0 37.0 80-81 33.692744189721765 37.0 33.0 37.0 27.0 37.0 82-83 33.59706350068647 37.0 33.0 37.0 27.0 37.0 84-85 33.46684215205002 37.0 33.0 37.0 27.0 37.0 86-87 33.350256314016775 37.0 33.0 37.0 27.0 37.0 88-89 33.21846448643363 35.0 33.0 37.0 27.0 37.0 90-91 33.050447932202715 33.0 33.0 37.0 27.0 37.0 92-93 32.94370968857106 33.0 33.0 37.0 27.0 37.0 94-95 32.865240065979876 33.0 33.0 37.0 27.0 37.0 96-97 32.7085625672886 33.0 33.0 37.0 27.0 37.0 98-99 32.56927392511088 33.0 33.0 37.0 27.0 37.0 100-101 31.666683128710133 33.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.29190951676326193 2306 2 -0.02762649966784636 2306 3 -0.06126502447095561 2306 4 0.33252369300340234 2306 5 0.4238745189650004 2306 6 0.40611066297064724 2306 7 0.4857837714512243 2306 8 0.38613818899391106 2306 9 0.21818505246990583 2306 10-11 -0.19734477077244605 2306 12-13 0.24958962285234776 2306 14-15 0.2395318762698153 2306 16-17 0.6983288758206285 2306 18-19 0.6278424986620195 2306 20-21 0.723886986302098 2306 22-23 0.20818280812355994 2306 24-25 0.6786299265957112 2306 26-27 0.7697751538722954 2306 28-29 0.609395020639866 2306 30-31 0.5595148357892086 2306 32-33 0.47266687447349653 2306 34-35 1.1060565388999848 2306 36-37 0.02215328474409972 2306 38-39 0.6754962050797815 2306 40-41 0.9006604305421817 2306 42-43 0.41303985594171166 2306 44-45 1.3047529994912992 2306 46-47 0.8336849945653029 2306 48-49 0.9641838020745652 2306 50-51 0.6339703202899685 2306 52-53 0.5443396741213533 2306 54-55 0.9545662961685153 2306 56-57 1.4985761504064143 2306 58-59 0.7316137853100102 2306 60-61 0.9846207249892984 2306 62-63 1.5739741895790118 2306 64-65 0.8612020842800376 2306 66-67 1.0332479461262878 2306 68-69 1.2402317935311942 2306 70-71 1.5969822040428383 2306 72-73 1.599059659868729 2306 74-75 1.282457649025531 2306 76-77 1.2911595693799782 2306 78-79 1.1727052602965884 2306 80-81 1.5064851628973557 2306 82-83 1.028495763210735 2306 84-85 1.037054544261494 2306 86-87 1.3990639726018514 2306 88-89 1.1110067646775406 2306 90-91 1.201210083447556 2306 92-93 1.2534659018720262 2306 94-95 1.1876189370467714 2306 96-97 1.3886152778926473 2306 98-99 1.6279848514757802 2306 100-101 1.9731383090990668 2307 1 -0.10551298410751286 2307 2 0.08313630075012668 2307 3 0.17906935379446054 2307 4 -0.3320531095044359 2307 5 0.04769897037565585 2307 6 -0.33370124925505706 2307 7 -0.09798495024853082 2307 8 0.25795985391902576 2307 9 0.10951211621075885 2307 10-11 -0.2785011622736633 2307 12-13 -0.13633862514486594 2307 14-15 0.1573305526891815 2307 16-17 -0.035735909968288126 2307 18-19 -0.09977853930384839 2307 20-21 0.04398451329617359 2307 22-23 -0.22668309225958438 2307 24-25 -0.05404161642066185 2307 26-27 -0.15220325086473707 2307 28-29 -0.30718456835352015 2307 30-31 -0.08938798552740934 2307 32-33 0.22257979680021123 2307 34-35 -0.049638340932823155 2307 36-37 0.5775346849530862 2307 38-39 0.3411179396670363 2307 40-41 0.14360713900614286 2307 42-43 -0.46017505175596085 2307 44-45 -0.6170512498991627 2307 46-47 -0.1593487915719436 2307 48-49 -0.19046614567883324 2307 50-51 -0.44979840140979377 2307 52-53 -0.9939745021238551 2307 54-55 -0.22272384663887124 2307 56-57 -0.45004802235568775 2307 58-59 -1.138292170802849 2307 60-61 -0.3144040050699175 2307 62-63 -0.5477603976728247 2307 64-65 -0.10588255521839329 2307 66-67 -0.4871282294223036 2307 68-69 -0.8062676840027692 2307 70-71 -0.6363859603598101 2307 72-73 -0.15642623354819563 2307 74-75 -0.4362814662653065 2307 76-77 0.18144170104142887 2307 78-79 0.4550110770851674 2307 80-81 0.060473320333784386 2307 82-83 0.3222958328728751 2307 84-85 -0.5413153616946147 2307 86-87 -0.3529388138836964 2307 88-89 -0.4464644996902791 2307 90-91 -0.021186294121228855 2307 92-93 0.04117053440425167 2307 94-95 -0.28016232383793493 2307 96-97 0.3400259361998579 2307 98-99 0.40018965816334884 2307 100-101 0.08808083783469556 2201 1 0.01147320430725074 2201 2 -0.15464972275017885 2201 3 0.054146657443190804 2201 4 0.31235010632735083 2201 5 0.16396365806891566 2201 6 0.1666079653398711 2201 7 0.1798958990608881 2201 8 0.4987349518369797 2201 9 0.3896605392163295 2201 10-11 0.4919585367574584 2201 12-13 0.1252053863991449 2201 14-15 0.19062265740986106 2201 16-17 0.5972263658511281 2201 18-19 0.38347233680417503 2201 20-21 0.1504058697175381 2201 22-23 0.43261395989460283 2201 24-25 0.3968447987514665 2201 26-27 0.48488188622039985 2201 28-29 0.7559467447777948 2201 30-31 0.6890597572060457 2201 32-33 0.32441447006345214 2201 34-35 0.639600991163654 2201 36-37 0.7409545983730013 2201 38-39 0.005310889574268174 2201 40-41 0.4772496869343996 2201 42-43 0.25323455451079013 2201 44-45 0.32146658147581064 2201 46-47 0.3027208847834544 2201 48-49 0.4859407807281002 2201 50-51 0.48624571320575427 2201 52-53 0.7775674479895187 2201 54-55 0.3813784003205214 2201 56-57 0.5219165210374257 2201 58-59 0.08975359295720153 2201 60-61 -0.08522445446179461 2201 62-63 0.12493595359495657 2201 64-65 0.11999870637715304 2201 66-67 0.5428655862857994 2201 68-69 0.4220872943522167 2201 70-71 -0.2908901862926072 2201 72-73 -0.2935629063991527 2201 74-75 -0.6088747460014474 2201 76-77 -0.6633325723042809 2201 78-79 -0.3086459008575275 2201 80-81 -0.440969681109209 2201 82-83 0.35795858282133963 2201 84-85 0.3666932963140397 2201 86-87 0.2800749977015471 2201 88-89 0.2355082894253684 2201 90-91 0.26524949231455963 2201 92-93 0.07260735155535514 2201 94-95 0.3845459837275129 2201 96-97 0.69268518171625 2201 98-99 0.19595341821282375 2201 100-101 -0.500354536314962 1101 1 0.18741630882178129 1101 2 0.143742361356189 1101 3 -0.06335488862978167 1101 4 0.1729973955460693 1101 5 0.10830503098171818 1101 6 0.05013713458332347 1101 7 0.10403525177166983 1101 8 0.318565914525081 1101 9 0.3923403990390426 1101 10-11 -0.08658197035447301 1101 12-13 -0.005025493831738004 1101 14-15 0.2532901486487731 1101 16-17 -0.11149348572586604 1101 18-19 -0.37755631708161985 1101 20-21 0.10964107895274111 1101 22-23 0.061195695619197465 1101 24-25 0.09242303004398877 1101 26-27 0.10032200166052263 1101 28-29 -0.276881538050489 1101 30-31 -0.5237314198708489 1101 32-33 0.21247878669920084 1101 34-35 -0.2516585429530309 1101 36-37 -0.1345865271681248 1101 38-39 -0.14878105023195332 1101 40-41 -0.2402312448322519 1101 42-43 0.35800676642585927 1101 44-45 -8.896337375432495E-4 1101 46-47 -0.3916720238951683 1101 48-49 -0.554102509315193 1101 50-51 0.19666624505484975 1101 52-53 -0.2970048051541596 1101 54-55 -0.13686526078029004 1101 56-57 -0.2581288304364975 1101 58-59 0.16978863727796778 1101 60-61 0.029030338364421482 1101 62-63 -0.3608917108041325 1101 64-65 0.11633966700382103 1101 66-67 -0.22450196679603351 1101 68-69 0.02201514428006135 1101 70-71 -0.045476869450716606 1101 72-73 -0.1715777486997112 1101 74-75 0.11422358423974543 1101 76-77 0.4137649333646536 1101 78-79 0.7428898649639493 1101 80-81 0.2119884718489402 1101 82-83 0.10512411570115887 1101 84-85 0.5091896888104372 1101 86-87 -0.16101962196450614 1101 88-89 0.1898991366733611 1101 90-91 0.3373995644646257 1101 92-93 -0.08004158680787299 1101 94-95 0.1996356559600443 1101 96-97 0.6986117947857124 1101 98-99 0.2638260217997086 1101 100-101 0.47191922167307965 2304 1 -0.031697910292436404 2304 2 0.09770506531889112 2304 3 0.13303205775716265 2304 4 0.2279702005188753 2304 5 0.17299034566703142 2304 6 -0.19092735648116843 2304 7 -0.0033851056486824405 2304 8 0.008154104113273775 2304 9 0.2895820462806924 2304 10-11 1.0217882967822334E-4 2304 12-13 0.2227328589266122 2304 14-15 0.024511982370611918 2304 16-17 0.3041533674209873 2304 18-19 -0.583364397889703 2304 20-21 0.008922353234019909 2304 22-23 0.008359642783148047 2304 24-25 -0.5534103032893469 2304 26-27 -0.12020053136201625 2304 28-29 0.04921597554702828 2304 30-31 -0.4061129272523587 2304 32-33 0.0745125862330056 2304 34-35 -0.4508621171565963 2304 36-37 -0.29819375327534914 2304 38-39 0.2069396179887164 2304 40-41 -4.779425789465108E-4 2304 42-43 0.048760457179547245 2304 44-45 -0.12545257080048344 2304 46-47 0.34147677175361935 2304 48-49 0.1322319895193047 2304 50-51 -0.03789086450225909 2304 52-53 0.43818361753426416 2304 54-55 0.6666021051870814 2304 56-57 0.26377287896521295 2304 58-59 -0.006007788518466839 2304 60-61 0.30773080456489055 2304 62-63 0.3549202550078334 2304 64-65 -0.15570773004357363 2304 66-67 -0.3111375534316281 2304 68-69 -0.0600555627906445 2304 70-71 0.21656664259280234 2304 72-73 -0.039147678769644756 2304 74-75 -0.2144477444315882 2304 76-77 -0.4826025880028624 2304 78-79 -0.446989699915612 2304 80-81 -0.08657407671360318 2304 82-83 -0.1966921236150796 2304 84-85 -0.17039461577387272 2304 86-87 -0.39790773385261957 2304 88-89 -0.04902860225437422 2304 90-91 0.3215681736332314 2304 92-93 0.11420860744232186 2304 94-95 0.31880815013253283 2304 96-97 0.03422756790148895 2304 98-99 0.17976425023794462 2304 100-101 0.24032442757828676 2202 1 -0.0701594487539765 2202 2 -0.16591672955290448 2202 3 -0.37017307044796155 2202 4 0.09976507231375109 2202 5 0.21625957643626492 2202 6 5.790537752474734E-4 2202 7 -0.038641515905098345 2202 8 -0.06716640870723722 2202 9 -0.1992000050013587 2202 10-11 -0.11167666732416848 2202 12-13 -0.04890175645800099 2202 14-15 0.14470429006291852 2202 16-17 -0.01916394027131929 2202 18-19 0.44267726877696134 2202 20-21 0.3126082506699177 2202 22-23 -0.20237753670402725 2202 24-25 -0.016951969956011226 2202 26-27 0.36136998145850185 2202 28-29 -0.2857199218888695 2202 30-31 0.023774893260466 2202 32-33 -0.3093015163311037 2202 34-35 -0.5378012197206985 2202 36-37 -0.724075163531765 2202 38-39 -0.3122911512420572 2202 40-41 -0.0012792246302311128 2202 42-43 0.042350200769291746 2202 44-45 -0.36784039131330104 2202 46-47 -0.15051040773355595 2202 48-49 -0.3741782668909437 2202 50-51 0.166836699600303 2202 52-53 0.07199772009835925 2202 54-55 0.9085892846742638 2202 56-57 -0.046323274880940346 2202 58-59 -0.14823535262102183 2202 60-61 0.5437083686674598 2202 62-63 0.4342471780847532 2202 64-65 0.20567047508463787 2202 66-67 0.05264449785043013 2202 68-69 0.2889027705426841 2202 70-71 0.42169484772100674 2202 72-73 -0.10765729415425795 2202 74-75 0.2174432812094338 2202 76-77 0.4849454889202107 2202 78-79 -0.1545217511976702 2202 80-81 0.20709579508125842 2202 82-83 0.4315130045900517 2202 84-85 0.1925861534569009 2202 86-87 0.43462431742943863 2202 88-89 0.13166050030972798 2202 90-91 0.319564968505027 2202 92-93 0.7103624535961686 2202 94-95 0.041965201414591036 2202 96-97 -0.6841217910728687 2202 98-99 -0.8398671600184677 2202 100-101 0.015164171168027707 1102 1 -0.22015944875397508 1102 2 0.2761666037804318 1102 3 0.07149359621870133 1102 4 0.3564317389804188 1102 5 -0.16624042356373536 1102 6 0.01599572044191433 1102 7 0.049691817428239915 1102 8 -0.027999742040570652 1102 9 0.4149666616653036 1102 10-11 -0.13855166732417246 1102 12-13 0.1423482435419956 1102 14-15 -0.0702957099370849 1102 16-17 -0.011038940271319575 1102 18-19 0.061635602110293064 1102 20-21 0.13469158400324233 1102 22-23 0.34220579662930106 1102 24-25 0.1440896967106582 1102 26-27 0.18345331479182647 1102 28-29 0.020946744777795345 1102 30-31 0.17273322659379886 1102 32-33 0.027781817002228593 1102 34-35 0.3049071136126358 1102 36-37 0.7123831698015763 1102 38-39 0.0802088487579411 1102 40-41 0.3416374420364363 1102 42-43 0.6156835341026223 1102 44-45 0.3132012753533644 1102 46-47 0.4628229255997809 1102 48-49 0.24165506644238377 1102 50-51 0.31287836626697896 1102 52-53 0.4099143867650312 1102 54-55 0.4852559513409247 1102 56-57 0.8424267251190614 1102 58-59 0.2932229807123079 1102 60-61 0.6160000353341246 1102 62-63 0.5729971780847549 1102 64-65 1.0577538084179636 1102 66-67 0.758477831183761 1102 68-69 1.0199444372093538 1102 70-71 0.5850281810543336 1102 72-73 0.6629677058457375 1102 74-75 1.397859947876107 1102 76-77 1.1243204889202119 1102 78-79 0.3483949154690009 1102 80-81 0.8368874617479349 1102 82-83 0.5273463379233831 1102 84-85 0.108836153456906 1102 86-87 0.5467076507627695 1102 88-89 0.21020216697638716 1102 90-91 -0.076893364828301 1102 92-93 0.24536245359616515 1102 94-95 0.2884235347479205 1102 96-97 -0.1705801244062073 1102 98-99 0.1003411733148667 1102 100-101 0.11474750450136284 2305 1 0.018075845363668464 2305 2 -0.025598102101923814 2305 3 0.009728890336347717 2305 4 0.09486311152943472 2305 5 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4017.0 33 5680.0 34 8317.0 35 13258.0 36 22184.0 37 27514.0 38 7831.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.165746074721106 21.430194757072417 11.410728930960545 24.99333023724593 2 16.209675826238122 20.11121866419075 39.21099938760593 24.468106121965192 3 19.851241628637467 24.147059404571404 31.95835555818961 24.043343408601515 4 12.669517892595044 15.839119741611766 40.6823187775945 30.80904358819869 5 12.373201110200212 37.34085319478878 37.35764447912448 12.928301215886531 6 30.238139920784647 39.516806100174826 17.473800657823258 12.77125332121727 7 24.664421243937852 31.628853352824393 24.327607834615726 19.37911756862203 8 23.942396017502446 37.38925160257993 21.03947927264107 17.62887310727655 9 22.606995051509735 14.856335746668906 22.485505170727855 40.051164031093506 10-11 25.73610027359916 24.729610936064716 29.420305601374906 20.113983188961214 12-13 23.494463814782257 22.656875043212864 30.377902669814212 23.47075847219067 14-15 22.40648736208923 24.75973647560819 24.516262852740436 28.31751330956214 16-17 22.28993609434726 29.94676175142973 26.191934257183213 21.571367897039796 18-19 21.48296672362534 29.435615301798645 28.180219867052536 20.901198107523484 20-21 22.07955117884693 27.631046097014117 27.88933555900161 22.400067165137344 22-23 22.15758126487757 27.48338156712069 28.23750777831554 22.121529389686202 24-25 22.412413697737126 27.94316644113667 27.925881295496975 21.718538565629228 26-27 22.099305631006587 27.836492399474533 28.015270191519413 22.048931777999467 28-29 22.279071145659454 27.855259129026205 27.909090011161265 21.956579714153076 30-31 21.924972590697628 27.808836166451012 28.250348172219315 22.015843070632044 32-33 22.120047805774227 28.122438094485545 27.869581106841956 21.887932992898275 34-35 21.95015951720119 27.606353031814546 28.35158973953755 22.091897711446716 36-37 22.107701273174442 27.95798228025641 28.116017897533656 21.81829854903549 38-39 22.170915520085337 27.80735458253904 27.788093991683375 22.233635905692246 40-41 22.008435151072174 27.68981559218909 28.182195312268504 22.119553944470237 42-43 22.34919945082623 27.452268304969234 28.221210355283823 21.977321888920713 44-45 21.81928627164347 27.614254812678407 28.194047983564296 22.372410932113826 46-47 21.808421322955663 27.46510869887301 28.45974536511166 22.26672461305967 48-49 21.67952352261391 27.40535148109005 28.61185464674101 22.30327034955503 50-51 21.485436030145294 27.598945112254675 28.655808302796242 22.25981055480379 52-53 21.31406615766028 27.32583981114744 28.999041909070257 22.361052122122025 54-55 21.21973864859793 27.48239384451271 28.800015803561728 22.497851703327637 56-57 21.09577946129609 27.3164564463716 28.833104510929154 22.754659581403157 58-59 20.955522850962534 27.599438973558666 28.69334176189959 22.75169641357921 60-61 21.17825429906265 27.600920557470637 28.406408344280592 22.814416799186116 62-63 21.084420651304285 26.908033147970723 28.6469187993244 23.360627401400592 64-65 20.861195341900178 27.082366188279682 28.402457453848662 23.653981015971475 66-67 21.08886540304021 27.169285777782164 27.91007773376925 23.831771085408374 68-69 21.328881996780026 27.241389528164913 27.618205703110338 23.811522771944727 70-71 21.076025009136433 27.164347164742253 27.77574745908359 23.98388036703772 72-73 20.735754570686368 26.946060468378057 28.017245636735378 24.30093932420019 74-75 21.1639323212469 26.906551564058752 27.439921772369445 24.489594342324903 76-77 21.16047529211896 27.182620032989934 27.292257242476026 24.364647432415083 78-79 21.11701549736772 27.090267969143543 27.350039015043016 24.44267751844572 80-81 20.94663334749069 26.905069980146774 27.14162954475865 25.006667127603883 82-83 20.91403850142726 26.898155921890897 27.4828877058167 24.704917870865145 84-85 21.010835317009573 26.72184743636597 27.47893681538477 24.78838043123969 86-87 21.16788321167883 26.776172179805023 27.434983159329533 24.62096144918661 88-89 21.057752140888752 26.449729857866718 27.551534427071502 24.94098357417303 90-91 21.069604812184544 26.451705303082683 27.39152336457829 25.08716652015448 92-93 21.037503827425105 26.022539829914166 27.549065120551546 25.390891222109186 94-95 21.140226978655313 25.776103039222463 27.706113015220808 25.377556966901416 96-97 21.531858992720483 26.207243957606945 27.08384777219166 25.17704927748091 98-99 21.119484803887676 25.92673073693984 27.453256027577215 25.50052843159527 100-101 21.03503452090515 25.543494365042523 27.88637239117766 25.535098722874665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 0.5 10 0.5 11 1.0 12 1.0 13 2.0 14 3.0 15 8.5 16 15.0 17 24.0 18 43.0 19 73.0 20 108.5 21 162.5 22 236.0 23 319.0 24 439.0 25 588.5 26 742.0 27 917.5 28 1104.5 29 1257.5 30 1457.0 31 1683.5 32 1904.0 33 2037.5 34 2141.5 35 2316.0 36 2520.5 37 2696.5 38 2828.5 39 2912.5 40 2995.5 41 3157.5 42 3219.0 43 3309.0 44 3507.5 45 3639.5 46 3747.0 47 3781.5 48 3786.0 49 3879.0 50 3992.5 51 3995.5 52 3903.5 53 3716.0 54 3423.0 55 3099.5 56 2717.5 57 2327.0 58 1919.5 59 1590.5 60 1369.0 61 1074.0 62 841.0 63 699.0 64 567.5 65 497.0 66 415.5 67 344.5 68 278.5 69 215.5 70 188.0 71 147.5 72 108.0 73 84.5 74 58.0 75 36.5 76 24.0 77 16.5 78 8.5 79 7.5 80 6.0 81 2.5 82 0.5 83 0.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039508904319310964 2 9.87722607982774E-4 3 0.0049386130399138705 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 101243.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.56392501633084 #Duplication Level Percentage of deduplicated Percentage of total 1 98.21352272100799 95.8209676634213 2 1.488929413708894 2.9053159534740796 3 0.18908293950264818 0.5534302119451137 4 0.04676923865200471 0.18251961971660277 5 0.014959540530737236 0.07297557453098048 6 0.012951891239804341 0.0758182407443966 7 0.011527585480255636 0.07872735397905034 8 0.004187117866314674 0.03268093228349313 9 0.0038603751912290726 0.03389700201227793 >10 0.014209176820132197 0.24366744789267955 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 9.87722607982774E-4 0.0 0.0 12-13 9.87722607982774E-4 0.0 0.0 14-15 9.87722607982774E-4 0.0 0.0 16-17 9.87722607982774E-4 0.0 0.0 18-19 9.87722607982774E-4 0.0 0.0 20-21 9.87722607982774E-4 9.87722607982774E-4 0.0 22-23 9.87722607982774E-4 9.87722607982774E-4 9.87722607982774E-4 24-25 9.87722607982774E-4 9.87722607982774E-4 0.001481583911974161 26-27 9.87722607982774E-4 9.87722607982774E-4 0.005926335647896645 28-29 9.87722607982774E-4 9.87722607982774E-4 0.02321148128759519 30-31 9.87722607982774E-4 9.87722607982774E-4 0.044447517359224836 32-33 9.87722607982774E-4 9.87722607982774E-4 0.06321424691089754 34-35 9.87722607982774E-4 9.87722607982774E-4 0.1101310707900793 36-37 9.87722607982774E-4 9.87722607982774E-4 0.2800193593631165 38-39 9.87722607982774E-4 9.87722607982774E-4 0.590658119573699 40-41 9.87722607982774E-4 9.87722607982774E-4 1.0455043805497664 42-43 9.87722607982774E-4 9.87722607982774E-4 1.5645526110447143 44-45 9.87722607982774E-4 9.87722607982774E-4 2.2732435822723547 46-47 9.87722607982774E-4 9.87722607982774E-4 3.0910779016820915 48-49 9.87722607982774E-4 9.87722607982774E-4 3.8466856967889136 50-51 9.87722607982774E-4 9.87722607982774E-4 4.527720434993036 52-53 9.87722607982774E-4 9.87722607982774E-4 5.2403622966526076 54-55 9.87722607982774E-4 9.87722607982774E-4 6.043874638246595 56-57 9.87722607982774E-4 9.87722607982774E-4 6.9135643945754275 58-59 9.87722607982774E-4 9.87722607982774E-4 7.804984048279881 60-61 9.87722607982774E-4 9.87722607982774E-4 8.618867477257687 62-63 9.87722607982774E-4 9.87722607982774E-4 9.412996454075838 64-65 9.87722607982774E-4 9.87722607982774E-4 10.251572948253212 66-67 9.87722607982774E-4 9.87722607982774E-4 11.177562893237063 68-69 9.87722607982774E-4 9.87722607982774E-4 12.06305621129362 70-71 9.87722607982774E-4 9.87722607982774E-4 12.90262042807898 72-73 9.87722607982774E-4 9.87722607982774E-4 13.69724326620112 74-75 9.87722607982774E-4 9.87722607982774E-4 14.495323133451201 76-77 9.87722607982774E-4 9.87722607982774E-4 15.328467153284672 78-79 9.87722607982774E-4 9.87722607982774E-4 16.142844443566467 80-81 9.87722607982774E-4 9.87722607982774E-4 16.937467281688612 82-83 9.87722607982774E-4 9.87722607982774E-4 17.73851031676264 84-85 9.87722607982774E-4 9.87722607982774E-4 18.523749790108944 86-87 9.87722607982774E-4 9.87722607982774E-4 19.311952431279202 88-89 9.87722607982774E-4 9.87722607982774E-4 20.09817962723349 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACA 35 2.7759236E-4 54.285152 4 GTATTAA 45 9.560645E-4 42.242653 1 GACTCCA 45 9.560645E-4 42.242653 2 TAAGACA 45 9.583949E-4 42.221783 4 CTGTGCG 45 9.583949E-4 42.221783 9 CTCTATC 50 0.0016050373 38.01839 2 TACTTGT 55 0.0025687015 34.545097 7 TTATAGA 55 0.0025687015 34.545097 4 GAGGGAA 55 0.0025687015 34.545097 7 ACCGATC 60 0.0039342963 31.666338 8 GCTGCAC 80 4.4653317E-4 29.687191 3 CACTAAT 65 0.005805497 29.244913 2 TATTATA 65 0.005805497 29.244913 2 CGCTTAT 65 0.005805497 29.244913 1 ATTATAG 70 0.008356514 27.142576 3 TGCACTG 70 0.008356514 27.142576 5 CTTTATC 130 0.0074958126 18.27807 2 TCTGCTT 150 1.4516445E-6 15.833168 94-95 CTTCTGC 180 9.61453E-6 13.194307 92-93 CCGTCTT 280 3.5142875E-9 12.723083 94-95 >>END_MODULE