FastQCFastQC Report
Wed 17 Sep 2014
G50_MOV_D1_37_TCCTGAGC-AAGGAGTA_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameG50_MOV_D1_37_TCCTGAGC-AAGGAGTA_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101243
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACA352.7759236E-454.2851524
GTATTAA459.560645E-442.2426531
GACTCCA459.560645E-442.2426532
TAAGACA459.583949E-442.2217834
CTGTGCG459.583949E-442.2217839
CTCTATC500.001605037338.018392
TACTTGT550.002568701534.5450977
TTATAGA550.002568701534.5450974
GAGGGAA550.002568701534.5450977
ACCGATC600.003934296331.6663388
GCTGCAC804.4653317E-429.6871913
CACTAAT650.00580549729.2449132
TATTATA650.00580549729.2449132
CGCTTAT650.00580549729.2449131
ATTATAG700.00835651427.1425763
TGCACTG700.00835651427.1425765
CTTTATC1300.007495812618.278072
TCTGCTT1501.4516445E-615.83316894-95
CTTCTGC1809.61453E-613.19430792-93
CCGTCTT2803.5142875E-912.72308394-95
Produced by FastQC (version 0.11.2)